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Tommos Cecilia C Graduate Group in Biochemistry and Molecular Biophysics and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6059, United States. - - 2013
2-Mercaptophenol-α₃C serves as a biomimetic model for enzymes that use tyrosine residues in redox catalysis and multistep electron transfer. This model protein was tailored for electrochemical studies of phenol oxidation and reduction with specific emphasis on the redox-driven protonic reactions occurring at the phenol oxygen. This protein contains a covalently ...
Fu Yi Y Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 W. Lombard Street, Baltimore, Maryland 21201, USA. - - 2013
It has been suggested that narrow gaps between metallic nanostructures can be practical for producing large field enhancement. We design a hybrid silver nanostructure geometry in which fluorescent emitters are sandwiched between silver nanoparticles and silver island film (SIF). A desired number of polyelectrolyte layers are deposited on the SIF ...
Goulah Christopher C CC Hauptman-Woodward Medical Research Institute, The State University of New York at Buffalo, New York 14203, United - - 2013
α-Dioxygenases (α-DOX) oxygenate fatty acids into 2(R)-hydroperoxides. Despite the low level of sequence identity, α-DOX share common catalytic features with cyclooxygenases (COX), including the use of a tyrosyl radical during catalysis. We determined the X-ray crystal structure of Arabidopsis thaliana α-DOX to 1.5 Å resolution. The α-DOX structure is monomeric, ...
Geserick Claudia C Department Cell Biology, Plant Physiology, University of Salzburg, Hellbrunnerstr. 34, Salzburg, 5020, - - 2013
Numerous nucleotide sugars are needed in plants to synthesize cell wall polymers and glycoproteins. The de novo synthesis of nucleotide sugars is of major importance. During growth, however, some polymers are broken down to monosaccharides. Reactivation of these sugars into nucleotide sugars occurs in two steps: first, by a substrate-specific ...
Pierson Nicholas A NA Department of Chemistry, Indiana University, Bloomington, Indiana 47405, - - 2013
A recent ion mobility-mass spectrometry (IM-MS) study of the nonapeptide bradykinin (BK, amino acid sequence Arg(1)-Pro(2)-Pro(3)-Gly(4)-Phe(5)-Ser(6)-Pro(7)-Phe(8)-Arg(9)) found evidence for 10 populations of conformations that depend upon the solution composition [J. Am. Chem. Soc. 2011, 133, 13810]. Here, the role of the three proline residues (Pro(2), Pro(3), and Pro(7)) in establishing ...
Michaelis Vladimir K VK Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, - - 2013
We report direct (13)C dynamic nuclear polarization at 5 T under magic-angle spinning (MAS) at 82 K using a mixture of monoradicals with narrow EPR linewidths. We show the importance of optimizing both EPR linewidth and electron relaxation times by studying direct DNP of (13)C using SA-BDPA and trityl radical, ...
Min Kyoung Ah KA Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, United - - 2013
Understanding how a magnetic field affects the interaction of magnetic nanoparticles (MNPs) with cells is fundamental to any potential downstream applications of MNPs as gene and drug delivery vehicles. Here, we present a quantitative analysis of how a pulsed magnetic field influences the manner in which MNPs interact with and ...
Klinman Judith P JP Department of Chemistry, Department of Molecular and Cell Biology, and California Institute for Quantitative Biosciences (QB3), University of California , Berkeley, California 94720-3220, United - - 2013
Quantum tunneling and protein dynamics have emerged as important components of enzyme function. This review focuses on soybean lipoxygenase-1, to illustrate how the properties of enzymatic C-H bond activation link protein motions to the fundamental bond making-breaking processes.
Du Bingnan B Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, - - 2013
A palladium-catalyzed ortho-halogenation (I, Br, Cl) of arylnitrile is described. The optimal reaction conditions were identified after examining various factors such as catalyst, additive, solvent, and reaction temperature. Using cyano as the directing group, the halogenation reaction gave good to excellent yields. The method is compatible to the arylnitriles with ...
Hartyánszky István I Gottsegen György Országos Kardiológiai Intézet Gyermekszívközpont Budapest Haller. - - 2013
The surgical strategy to manage multilevel left ventricular outflow tract diseases is changing constantly, however, the Ross-procedure has remained a standard method for 45 years. The aim of the study was to analyze early and mid-term results of Ross-procedure in congenital heart defects (single surgeon's results). From 2001 until 2011 ...
Funai Katsuhiko K Division of Endocrinology, Metabolism and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, - - 2013
Exogenous dietary fat can induce obesity and promote diabetes, but endogenous fat production is not thought to affect skeletal muscle insulin resistance, an antecedent of metabolic disease. Unexpectedly, the lipogenic enzyme fatty acid synthase (FAS) was increased in the skeletal muscle of mice with diet-induced obesity and insulin resistance. Skeletal ...
Rousselle Anthony - - 2013
The ELR(+)-CXCL chemokines have been described typically as potent chemoattractants and activators of neutrophils during the acute phase of inflammation. Their role in atherosclerosis, a chronic inflammatory vascular disease, has been largely unexplored. Using a mouse model of atherosclerosis, we found that CXCL5 expression was upregulated during disease progression, both ...
Leavenworth Jianmei W - - 2013
Current therapies to treat autoimmune disease focus mainly on downstream targets of autoimmune responses, including effector cells and cytokines. A potentially more effective approach would entail targeting autoreactive T cells that initiate the disease cascade and break self tolerance. The murine MHC class Ib molecule Qa-1b (HLA-E in humans) exhibits ...
Kraft Matthew B MB Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United - - 2013
Mycobacteria and corynebacteria use decaprenylphosphoryl-β-D-arabinofuranose (DPA) as a critical cell wall building block. Arabinofuranosyltransferases that process this substrate to mediate cell wall assembly have served as drug targets, but little is known about the substrate specificity of any of these enzymes. To probe substrate recognition of DPA, we developed a ...
Cho Hoonsung H Center for Translational Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital, Charlestown, Massachusetts 02129, United - - 2013
Attaching DNA binding fluorochromes to nanoparticles (NPs) provides a way of obtaining NPs that bind to DNA through fluorochrome mediated interactions. To obtain a nanoparticle (NP) that bound to the DNA in biological systems, we attached the DNA binding fluorochrome, TO-PRO 1 (TO), to the surface of the Feraheme (FH) ...
Schwartz Ann V AV University of California, 185 Berry St., Suite 5700, San Francisco, CA 94107, USA. - - 2013
Factors that contribute to bone fragility in type 2 diabetes are not well understood. We assessed the effects of intensive glycemic control, thiazolidinediones (TZDs), and A1C levels on bone geometry and strength at the radius and tibia. In a substudy of the Action to Control Cardiovascular Risk in Diabetes trial, ...
Toney Michael D MD Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, California 95616, USA. - - 2013
The catalytic effects of perdeuterating the pyridoxal phosphate-dependent enzyme alanine racemase from Geobacillus stearothermophilus are reported. The mass of the heavy perdeuterated form is ~5.5% greater than that of the protiated form, causing kinetic isotope effects (KIEs) of ~1.3 on k(cat) and k(cat)/K(M) for both L- and D-alanine. These values ...
Scheele Dirk - - 2013
Although major depression is projected to be among the top three causes of disability-adjusted life years lost in 2030, relatively little is known concerning the extent to which depressed mood states can bias social-economic decision making away from optimal outcomes. One experimental framework to study the interaction between negative emotion ...
Wang Dongniu - - 2013
Hierarchical carbon encapsulated tin (Sn@C) embedded graphene nanosheet (GN) composites (Sn@C-GNs) have been successfully fabricated via a simple and scalable one-step chemical vapor deposition (CVD) procedure. The GN supported Sn@C core-shell structures consist of a crystalline tin core, which is thoroughly covered by a carbon shell and more interestingly, extra ...
Lövdahl S - - 2013
Sweden has been a pioneer in the treatment of haemophilia, with the first concentrate available in the 1950s. Treatment has improved over the years to its current state-of-the art. The aim of the current study was to evaluate the long-term outcome of haemophilia in terms of incidence, morbidity and mortality. ...
Skaaby Tea - - 2013
Vitamin D is associated with cardiovascular disease and renal function but the mechanisms are as yet unexplained. Microalbuminuria is associated with a higher risk of kidney function loss, cardiovascular disease, and mortality. Parathyroid hormone is a predictor of cardiovascular mortality and negatively correlated with glomerular filtration rate. We investigated the ...
Yee S - - 2013
The purpose of this study was to survey the current state of oncology sperm banking services provided by fertility clinics across Canada. A total of 78 Canadian fertility facilities were invited to complete a questionnaire related to the availability, accessibility, affordability and utilisation of sperm banking services for cancer patients. ...
Demiral S - - 2013
The purpose of this study is to evaluate the use of linear accelerator (LINAC)-based stereotactic body radiotherapy (SBRT) boost with multileaf collimator technique after pelvic radiotherapy (RT) in patients with endometrial cancer. Consecutive patients with endometrial cancer treated using LINAC-based SBRT boost after pelvic RT were enrolled in the study. ...
Liu Ziru - - 2013
The objective of this study was to quantify transport of Eu colloids in the vadose zone at the semi-arid Hanford site. Eu-hydroxy-carbonate colloids, Eu(OH)(CO3) were applied to the surface of field lysimeters and migration of the colloids through the sediments was monitored using wick samplers. The lysimeters were exposed to ...
Vukmirović Nenad - - 2013
The comparison of hole density of states (DOS) and hole mobilities of several organic polymer based systems was performed to gain insight into the main factors that determine the electrical properties of conjugated polymers. The DOS and the mobility of the systems under investigation were evaluated using an atomistic multiscale ...
Ogaki Soichiro - - 2013
The studies of differentiation of mouse or human embryonic stem (ES) cells into specific cell types of the intestinal cells would provide insights to the understanding of intestinal development and ultimately yield cells for the use in future regenerative medicine. Here, using an in vitro differentiation procedure of pluripotent stem ...
Meija Laila - - 2013
Higher intake of lignans, diphenolic plant compounds, may reduce the risk of certain types of cancer and cardiovascular diseases. We assessed the dietary intake of four lignans: matairesinol, secoisolariciresinol, lariciresinol and pinoresinol. Furthermore, for the breads we supplemented the data with two more lignans: syringaresinol and medioresinol. Study subjects were ...
Wang Lei - - 2013
Tufted angioma represents a rare vascular tumor that can develop in children and young adults. It usually presents as solitary plaque or large tumor on the extremities, trunk or other anatomic sites. Histopathologically, it is characterized by proliferation of closely packed capillaries in the dermis in a 'cannonball' distribution. We ...
Chang Ting-Chen - - 2013
Abstract Context: Oxidative stress may contribute to lymphedema and subsequent tissue damage. However, the causal role of oxidative stress in lymphedema remains unclear. Objective: We attempted to detect and identify the free radicals formed in lymphedema fluid and assessed the protective mechanisms and effects of specific enzyme inhibitors and natural ...
Shin Woo-Jin - - 2013
The emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus and the recent global circulation of H1N1 swine-origin influenza virus in 2009 have highlighted the need for new anti-influenza therapies. This has been made all the more important with the emergence of antiviral-resistant strains. Recent progress in achieving three-dimensional ...
Zhou C H - - 2013
The purpose of the present study was to determine the expression of Sca-1, the stem cell marker, among various lung cancers and evaluate their utility. Immunohistochemistry was done in 151 surgically resected lung cancer specimens or biopsies, including 76 cases of adenocarcinoma, 50 cases of squamous cell carcinoma, 15 cases ...
Qin Xu - - 2013
Based on the synergetic effect of the electrochemical accumulation process and the signal amplification of enzymes, a new sensitive method has been developed for the detection of subnanomolar melamine. There are two steps involved in the sensor construction process: (1) the accumulation of melamine on an electrode by cyclic voltammetric ...
Singh Manmeet - - 2013
The management of patients with drug eluting stents (DES) who require early surgical intervention prior to the completion of antiplatelet therapy is challenging. Available literature suggests that bridging these high risk patients with glycoprotein IIb/IIIa (g2b3a) inhibitors could be efficacious in preventing stent thrombosis. [1] However, this still remains to ...
Nishi Masayasu - - 2013
We shed light on the specific hydration structure around a zinc ion of nanosolution restricted in a cylindrical micropore of single-wall carbon nanotube (SWNT) by comparison with the structure restricted in a cylindrical mesopore of multi-wall carbon nanotube (MWNT) and that of bulk aqueous solution. The average micropore width of ...
Singer E E Department of Nephrology, Campus Mitte, Charité - Universitätsmedizin Berlin, Berlin, - - 2013
Neutrophil gelatinase-associated lipocalin (NGAL), a 25 kDa protein produced by injured nephron epithelia, is one of the most promising new markers of renal epithelial injury. In contrast to serum creatinine and urinary output, which are the measures of kidney function, NGAL is specifically induced in the damaged nephron and then released ...
Sharmin Tanjina - - 2013
Pyriproxyfen is a juvenile hormone mimic of vital importance for insect development with little risk to humans. This study was performed to investigate whether high doses of pyriproxyfen affect the immune response in mammals. Mice were immunized thrice with OVA in 5% ethanol, with or without pyriproxyfen or alum. Pyriproxyfen ...
Lai Francesco - - 2013
Development of drug delivery systems for brain delivery is one of the most challenging research topics in pharmaceutical areas, mainly due to the presence of the blood-brain barrier (BBB), which separates the blood from the cerebral parenchyma thus limiting the brain uptake of the majority of therapeutic agents. Among the ...
Kosari-Nasab Morteza M Laboratory of Plant Biotechnology, Hayyan Research Institute, University of Tabriz, Tabriz, - - 2013
Neuropsychiatric disorders, like anxiety and depression, are global problems for clinical researchers in neurology. Recently, some authors have shown neuroprotective and anti-inflammatory effects of Scrophularia striata Boiss (Scrophulariaceae) extract in rodents. The purpose of the current study was to investigate the effects of S. striata extract on anxiety and depressant-like ...
Börsch Michael - - 2013
Optical microscopy of single F(1) -ATPase and F(o) F(1) -ATP synthases started 15 years ago. Direct demonstration of ATP-driven subunit rotation by videomicroscopy became the new exciting tool to analyze the conformational changes of this enzyme during catalysis. Stimulated by these experiments, technical improvements for higher time resolution, better angular ...
Gotoh Ryo O - - 2013
Simple sequence repeats (SSRs) have become one of the most popular molecular markers for population genetic studies. The application of SSR markers has often been limited to source species because SSR loci are too labile to be maintained in even closely related species. However, a few extremely conserved SSR loci ...
Wang Huan - - 2013
The orientation factor κ(2) ranging from 0 to 4, which depends on the relative orientation of the transition dipoles of the energy donor (D) and the energy acceptor (A) in space, is one of the pivotal factors deciding the efficiency and directionality of resonance energy transfer (RET) in a D-A ...
Senet Patricia - - 2013
Biatain and Biatain-Ag are two identical wound dressings except the fact that Biatain-Ag releases silver. In the present multinational double-blinded randomised controlled trial the effect of the two dressings were compared for treatment of venous leg ulcers. A total of 181 patients were treated for 6 weeks with either Biatain or ...
Zak Krzysztof K Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Kraków, - - 2013
One of the hallmarks of cancer cells is the inactivation of the p53 pathway either due to mutations in the p53 gene or over-expression of negative regulators, Mdm2 and/or MdmX. Pharmacological disruption of the Mdm2/X-p53 interaction to restore p53 activity is an attractive concept, aiming at a targeted and non-toxic ...
Villa L - - 2013
BACKGROUND AND PURPOSE: α(1) -adrenoceptor (-AR) antagonists may facilitate ureter stone passage in humans. We aimed to study effects by the α(1A) -AR selective antagonist silodosin (compared to tamsulosin and prazosin) on ureter pressures in a rat model of ureter obstruction, and on contractions of human and rat isolated ureters. ...
Zhang Hai-Liang - - 2013
The skeleton is the most common metastatic organ in patients with prostate cancer (PCa). Non-invasive biomarkers that can facilitate the detection and monitoring of bone metastases are highly desirable. We designed this study to assess the expression patterns of serum miR-141 in patients with bone-metastatic PCa. Serum samples were collected ...
Ku Janice J-Y - - 2013
To assess the accuracy of grading diabetic retinopathy (DR) using single-field digital fundus photography compared with clinical grading from a dilated slit-lamp fundus examination in Indigenous Australians living in Central Australia. Cross-sectional study comparing DR grades in participants with diabetes mellitus presenting for examination at remote community clinics from 1 July ...
Park Youn Hee - - 2013
ABSTRACT: BACKGROUND: Hypothermic protection against ischemic stroke has been reported by many studies. Hypothermia is supposed to mitigate the effects of deleterious genes and proteins and promote the activity of protective genes and proteins in the ischemic brain. Metallothionein (MT)-1/2 is thought to be a crucial factor for metal homeostasis, ...
Kai K K 1] Breast Cancer Translational Research Laboratory, The University of Texas MD Anderson Cancer Center, Houston, TX, USA [2] Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA [3] Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Tokyo, - - 2014
Intratumoral heterogeneity within individual breast tumors is a well-known phenomenon that may contribute to drug resistance. This heterogeneity is dependent on several factors, such as types of oncogenic drivers and tumor precursor cells. The purpose of our study was to engineer a mouse mammary tumor model with intratumoral heterogeneity by ...
Aydemir Gamze - - 2013
Dietary consumption of tomato products and especially the red tomato pigment lycopene has been associated with lower risk of cancer. New evidence is emerging toward metabolic pathways mediating the anti-cancer activities of lycopene. In this review, we explore associations between tomatoes and lycopene intake and cancer and relate this to ...
Cahill S - - 2013
BACKGROUND: Falls are a significant and recurrent problem for individuals with intellectual disability (ID). There has been little exploration of the fall event from the perspective of the individual who falls or their carers. Research has focused predominantly on personal risk factors, leaving the behavioural and contextual analysis of falls ...
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