We present a case of acute uterine inversion in the third stage of labor in which critical management decisions were facilitated by ultrasound imaging in the operating room. Identification of the ovary and adnexa pulled into the indentation of the inversion allowed the successful diagnosis and guidance for uterine replacement. ...

The purpose of this study was to evaluate how psychological stress, gender and cortisol response to stress relate to risk behavior among 132 14-18 year old adolescents. Participants completed a laboratory based risk task prior to and immediately after a computerized psychological stress task, and salivary cortisol was collected from pre-stress ...

Microtubule-associated protein 1B (MAP1B) is a classical high molecular mass microtubule-associated protein expressed at high levels in the brain. It confers specific properties to neuronal microtubules and is essential for neuronal differentiation, brain development and synapse maturation. Misexpression of the protein contributes to the development of brain disorders in humans. ...

During a synthesis of 5-amino-4-(6-methoxy-2-methylpyridin-3-yl)-3-methyl-1H-pyrazole-1-carboxamide (see Scheme 1), a side-reaction produced 3-amino-4-(6-methoxy-2-methylpyridin-3-yl)-5-methyl-1H-pyrazole-1-carboxamide as a by-product that forms an equilibrium with the target-compound. The structure of the by-product was elucidated by the interpretation of 1D and 2D (HMQC, HMBC) NMR data where (1) H-(15)  N HMBC correlations revealed the position of carbamoyl ...

For recombinant protein production in E. coli fed-batch cultures, post-induction conditions have great influence in the quantity and quality of the product. The present paper covers the effect of different factors affecting the cellular environment in recombinant aldolase (rhamnulose-1-phosphate aldolase, RhuA) production. An operational mode employing an exponential addition profile ...

A fast and novel strategy for efficient ionization of phosphopeptides in mixtures is reported, in which the sample is acidified to low pH to suppress the deprotonation of phosphate groups and then followed by direct analysis using liquid sample desorption electrospray ionization mass spectrometry (DESI-MS).

Aim. The aim of the study was to evaluate the role of intracoronary shunt during off-pump coronary artery bypass surgery in patients with isolated left anterior descending coronary artery lesion. Methods. Forty patients undergoing off-pump coronary artery bypass using the left internal mammary artery to bypass the left anterior descending ...

Luminescent silver nanoclusters were anchored by designed oligonucleotides. After hybridizing with human telomerase RNA template, the luminescence of the cluster decreased linearly with respect to the concentration of the complementary strand (25-250 nM). The cluster is therefore a potential candidate for human telomerase detection.

Abstract Background: Bullying is common among young students, and cyberbullying has increased due to the use of technology. This study investigates the prevalence of bullying and cyberbullying among high school students and the emotional effects of bullying on students. Methods: Students at East Chapel Hill High School, Chapel Hill, North ...

Following the IUCN 5th World Congress on Protected Areas in 2003, the then-President of Madagascar decided to increase the area of Madagascar's protected areas from 1.7 to 6 million ha. To combine the aims of protection and timber production, a new concept was developed through the establishment of community-based forest ...

Curcumin, an active polyphenol of the golden spice turmeric, is a highly pleiotropic molecule with the potential to modulate the biological activity of a number of signaling molecules. Traditionally, this polyphenol has been used in Asian countries to treat such human ailments as acne, psoriasis, dermatitis, and rash. Recent studies ...

The type III secretion apparatus (T3SA) is a multi-protein complex central to the virulence of many Gram-negative pathogens. Currently, the mechanisms controlling the hierarchical addressing of needle subunits, translocators and effectors to the T3SA are still poorly understood. In Shigella, MxiC is known to sequester effectors within the cytoplasm prior ...

Erythropoiesis-stimulating agents (ESAs) have become a hallmark of anaemia therapy in patients with chronic kidney disease (CKD). Although different ESAs are available for the treatment of renal anaemia, each nephrologist should select a single ESA for an individual patient. Epoetin alfa and epoetin beta have been used 1-3 times weekly ...

We describe two balloon-assisted techniques to facilitate the advancement of large, long sheaths through freshly placed stents or tortuous anatomy. The first technique uses a balloon inflated while partially housed within the sheath to help clear an edge, thus offering resistance. The second uses an angioplasty balloon inflated in a ...

Protein homeostasis in higher eukaryotes is balanced by a dynamic network of adaptive mechanisms, including the unfolded protein response (UPR) and autophagy. In a paper recently published in Cell Research, Zhu and co-workers uncover a novel biological function of the unspliced form of the UPR transcription factor XBP1 in the ...

The Behavior Assessment Scale for Children (BASC-2) is widely used to assess for internalizing/externalizing behavior problems; however, the role of its adaptive scales is not well established. This study examined the sensitivity of the parent version of the BASC-2 Adaptive Skills Composite (ASC) in identifying children with impairments on a ...

The objective of this study was to compare the expression levels of interleukin (IL)-17 IL-17 and IL-23 in serum from patients with both oral lichen planus and chronic periodontitis (OLP-CP), patients only with oral lichen planus (OLP), patients only with chronic periodontitis (CP), and healthy controls (HC). The serum samples ...

The study was promoted to probe into the effect of BLyS on the activity of peripheral B lymphocytes mediated by BLyS receptors in patients with systemic lupus erythematosus (SLE). Thirty new-onset patients having fulfilled the American College of Rheumatology (ACR) revised criteria for the diagnosis of SLE. Twenty age-matched healthy ...

Interstitial lung diseases (sometimes also referred to as interstitial pneumonias, or IPs) comprise a heterogeneous group of diffuse parenchymal lung diseases that are associated with identifiable causes (such as collagen vascular diseases (CVD-ILD), like rheumatoid arthritis and scleroderma, or chronic hypersensitivity pneumonitis) and unidentified etiologies (idiopathic interstitial pneumonias, IIP, the ...

Numerous families exhibiting both frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) have been described, and although many of these have been shown to harbour a repeat expansion in C9ORF72, several C9ORF72-negative FTD-ALS families remain. We performed neuropathological and genetic analysis of a large European Australian kindred (Aus-12) with autosomal ...

Emotional behavior is in part heritable and often disrupted in psychopathology. Identification of specific genetic variants that drive this heritability may provide important new insight into molecular and neurobiological mechanisms involved in emotionality. Our results demonstrate that the presynaptic vesicular monoamine transporter 1 (VMAT1) Thr136Ile (rs1390938) polymorphism is functional in ...

Background: Chronic diseases are considered major threats to self-rated health (SRH). In many elderly people multimorbidity is present, in elderly women more than in elderly men. This study aims at establishing the impact of multimorbidity and specific disease combinations on SRH in elderly women. Objectives: To study the relationship between ...

DeSouza R. Nursing Inquiry 2013 Regulating migrant maternity: Nursing and midwifery's emancipatory aims and assimilatory practices In contemporary Western societies, birthing is framed as transformative for mothers; however, it is also a site for the regulation of women and the exercise of power relations by health professionals. Nursing scholarship often ...

Outcome studies often examine the efficacy of sinus surgery with 1 year of follow-up data, while longer-term postoperative data is less easily available. This article reviews long-term outcomes of various endoscopic techniques to further delineate risk factors for surgical failure. A systematic review of the literature was performed and studies were ...

Aging is a complex process that is linked to an increased incidence of major diseases such as cardiovascular and neurodegenerative disease, but also cancer and immune disorders. MicroRNAs (miRNAs) are small non-coding RNAs, which post-transcriptionally control gene expression by inhibiting translation or inducing degradation of targeted mRNAs. MiRNAs target up ...

Ultrasonography is the standard modality to image the scrotum because it can provide information about volume, echo texture, tissue stiffness and functional information that includes macrovascularization and microvascularization. Indeed, ultrasound imaging is indicated in the presentation of acute scrotal pain and swelling to differentiate between testicular torsion, infarction and inflammation, ...

ABSTRACT Objective: The aim of this study is to identify the prevalence of Impulse Compulsive disorder (ICD) in PD patients and to study potential associative factors that may be related to the onset of ICD while on parkinsonian medications. Method: The study was conducted in two parts. In Part A, ...

Cardiac Ca(V)1.2 channels play a critical role in cardiac function. It has been proposed that the carboxyl-terminal intracellular tail of the Ca(V)1.2 channel is the target of Ca(2+)-dependent and Ca(2+)-independent regulation of the channel. Recent studies on C-terminal truncated forms of the Ca(V)1.2 channel reported neonatal death, reduced Ca(V)1.2 current, ...

Protein kinetic destabilization is a common feature to many human genetic diseases. Human phosphoglycerate kinase 1 (PGK1) deficiency is a rare genetic disease caused by mutations in the PGK1 protein, which often shows reduced kinetic stability. In this work, we have performed an in-depth characterization of the thermal stability of ...

Lin28 (also termed Lin28a) and Lin28b are related RNA-binding proteins, involved in the control of microRNA synthesis, especially of the let-7 family, with putative functions in early (embryo) development. However, their roles during postnatal maturation remain ill defined. Despite the general assumption that Lin28 and Lin28b share similar targets and ...

Intrinsic response properties of neurons change during network activity. These changes may reinforce the initiation of particular forms of network activity. If so, the involvement of neurons in particular behaviors in multifunctional networks could be determined by up or down regulation of their intrinsic excitability. Here we employed an experimental ...

Deregulated transforming growth factor-β (TGF-β) signaling in pancreatic cancer promotes tumor growth, invasion, metastasis, and a potent immunosuppressive network. A strategy for disrupting this tumor-promoting pathway is silencing TGF-β by small interfering RNA (siRNA). By introducing a triphosphate group at the 5' end of siRNA (ppp-siRNA) gene silencing can be ...

Time resolved confocal microscopy has been applied to study the cytoplasm and nucleus region of a single live Chinese hamster ovary (CHO) cell. To select the cytoplasm and the nucleus region, two different fluorescent probes are used. A hydrophobic fluorescent dye, DCM localizes preferentially in the cytoplasm region of a ...

A novel composite composed of TiSi(2), graphene and RuO(2) nanoparticles was fabricated by a one-pot deposition method using reduced graphene oxide (RGO) as a supporting matrix and RuCl(3) as the RuO(2) precursor. The resulting RuO(2)/TiSi(2)/RGO composite was characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectra, X-ray photoelectron ...

Objective: To present a case of Paget's disease of bone unmasked after vascular reperfusion.Methods: In this case study, we review the presentation, evaluation, diagnosis, and management of a patient with Paget's disease and peripheral vascular diseaseResults: A 79-year-old-woman with a history of coronary artery heart disease and recent finding of ...

BACKGROUND:Early neonatal mortality has remained high and unchanged for many years in Tanzania, a resource-limited country. Helping Babies Breathe (HBB), a novel educational program using basic interventions to enhance delivery room stabilization/resuscitation, has been developed to reduce the number of these deaths.METHODS:Master trainers from the 3 major referral hospitals, 4 ...

Preeclampsia is a pregnancy specific disorder and a risk factor for later cardiovascular disease. The cause and detailed pathophysiology remains unknown. G protein signaling is involved in a variety of physiological processes, including blood pressure regulation. We assessed whether distributions of 3 single nucleotide polymorphisms in genes coding for components ...

BACKGROUND: The aim of this study was to map pre-procedural variables for insertion of a central venous catheter, prophylactic blood component use and to investigate whether any independent variable could be identified as an independent risk factor for associated bleeding complications in patients outside the intensive care unit. METHODS: In ...

PURPOSE: Plasmacytoid dendritic cells (pDC) are antigen-presenting cells specialized in antiviral response. The measles virus vaccine (MV) is proposed as an antitumor agent to target and specifically kill tumor cells without infecting healthy cells. Experimental design: Here, we investigated, in vitro, the effects of MV-infected tumor cells on the phenotype ...

In laparoscopic colorectal resection, the medial-to-lateral approach has been largely adopted. This approach can be initiated by the division of either the inferior mesenteric artery (IMA) or the inferior mesenteric vein (IMV). This cadaveric study aimed to establish the feasibility of IMV dissection as the initial landmark of medial-to-lateral left ...

ABSTRACT: Background; Brain-computer interfaces (BCIs) were recently recognized as a method to promote neuroplastic effects in motor rehabilitation. The core of a BCI is a decoding stage by which signals from the brain are classified into different brain-states. The goal of this paper was to test the feasibility of a ...

BACKGROUND: Comparing and ranking hospitals based on health outcomes is becoming increasingly popular, although case-mix differences between hospitals and random variation are known to distort interpretation. The aim of this study was to explore whether surgical-site infection (SSI) rates are suitable for comparing hospitals, taking into account case-mix differences and ...

Objective: To review the effectiveness of alphaglucosidase inhibitor (AGI) therapy in the treatment of hypoglycemia after Roux-en-y gastric surgery.Methods: Retrospective case reviewResults: Four patients who previously underwent Roux-en-y gastric bypass were evaluated because of severe symptomatic post-prandial hypoglycemia unresponsive to a low carbohydrate diet. Mixed meal testing confirmed hyperinsulinemia. Other ...

This work describes an integrated approach for designing on demand Self-Assembled Monolayers (SAMs) on silicon oxides and particularly glass substrates for cell biology applications. Starting from commercially available compounds, the strategy relies on thiol-ene reaction and provides high quality SAMs exhibiting adhesive and anti-adhesive patterns.

Protein ubiquitination is important for cell signaling, DNA repair and proteasomal degradation, and it is not surprising that alterations in ubiquitination occur frequently in cancer. Ubiquitin conjugating enzymes (E2s) mediate ubiquitination by selective interactions with ubiquitin activating (E1) and ubiquitin ligase (E3) enzymes, and thus selective E2 small molecule inhibitor ...

Objective : The present study addresses gaps in the literature on affect-biased health perceptions by (a) investigating health bias while considering both valence and arousal components of affect; (b) establishing the presence of, and variability in, affective health bias at the daily level; and (c) exploring daily health bias in ...

Structured professional judgment (SPJ) has received considerable attention as an alternative to unstructured clinical judgment and actuarial assessment, and as a means of resolving their ongoing conflict. However, predictive validity studies have typically relied on receiver operating characteristic (ROC) analysis, the same technique commonly used to validate actuarial assessment tools. ...

Recent omics technologies provide information on multiple components of biological networks. Web-based data mining tools are continuously being developed. Because genes involved in specialized (secondary) metabolism are often co-ordinately regulated at the transcriptional level, a number of gene discovery studies have been successfully conducted using network analysis, especially by integrating ...

The Ly49 NK receptor family in mice is composed of several members that recognize MHC class I (MHC-I) or MHC-I-related molecules. We and others have shown before that Ly49E is a unique member, with a different expression pattern on NK cells and being triggered by the non-MHC-I-related protein urokinase plasminogen ...

This paper describes a method to control neuronal cell adhesion and differentiation with both chemical and topographic cues by using a spatially defined polymer brush pattern. First, biomimetic methacrylate polymer brushes containing tethered neurotransmitter acetylcholine functionalities in the form of dimethylaminoethyl methacrylate (DMAEMA), or free hydroxyl-terminated poly(ethylene glycol) (PEG) units ...