The differential diagnosis for the infant presenting with an apparent life-threatening event (ALTE) is broad. Toxic ingestions are a relatively uncommon cause of an ALTE, although several over-the-counter, prescription, and illicit drugs have been implicated. We present 2 cases of ethanol intoxication in infants as a previously unreported cause of ...

We report the results of a randomized clinical trial of a 3-hour, web-based, tobacco cessation education program, the Web-Based Respiratory Education About Tobacco and Health (WeBREATHe) program, for practicing pediatric respiratory therapists (RTs), registered nurses (RNs), and nurse practitioners (NPs). Two hundred fifteen RTs (n = 40), RNs (n = ...

2'-O-Neopentyldeoxyuridine (Un) was synthesized and incorporated into a series of oligodeoxyribonucleotides. Single and triple incorporations in various arrangements were performed. The Watson and Crick pairing properties with complementary DNA and RNA were investigated by UV melting curves, CD spectroscopy, and molecular dynamic simulations. The results were compared to those obtained ...

The emphasis on scholarship in nursing, demands for evidence-based practice, and attention to writing have raised the profile of academic writing within nursing curricula. This article provides a comprehensive review of English and writing course requirements across 81 English-language baccalaureate nursing programs in Canada. The data were gathered from a ...

Signaling lymphocytic activation molecule (SLAM)-associated protein (SAP) is a small adaptor molecule mutated in X-linked lymphoproliferative disease, a human immunodeficiency. It plays a critical role in initiation of T cell-dependent B cell responses leading to germinal center reaction, production of high affinity antibodies and B cell memory. However, whether SAP ...

The chiral ketone, (S)-3, shows high kinetic enantioselectivities toward L-form for general underivatized amino acids with hydrophobic side chains, and a high thermodynamic enantioselectivity toward D-form for cysteine with -SH polar side chain when used as an extractant in the enantioselective liquid-liquid extraction in the presence of Aliquat 336. Continuing ...

ObjectiveTo investigate the histopathologic changes in rat soft palate and base of tongue by performing experimental polycystic ovary syndrome (PCOS) and menopause models.Study DesignExperimental study.SettingThe study was conducted at the animal care facility of Haydarpasa Numune Education and Research Hospital.Subject and MethodsThirty healthy female Albino Wistar rats were divided into ...

SignificanceEarly signaling events leading to protection in the heart under cardiac injury are poorly understood. We identified one such protein, A kinase interacting protein (AKIP1), as a modulator that responds to oxidative stress; up-regulation of AKIP1 showed protection to ischemic injury through enhanced mitochondrial integrity. We show AKIP1 functions as ...

Lab-on-a-particle: Fluorescent, plasmonic, and magnetic SiO(2) *-Au-γ-Fe(2) O(3) nanostructures were assembled under continuous flow using two microfluidic devices (μR1 and μR2) connected in series. After assembling the SiO(2) *-Au nanostructures by electrostatic interactions, γ-Fe(2) O(3) nanoparticels were attached to the structures.

T cells from patients with systemic lupus erythematosus (SLE) produce insufficient amounts of the vital cytokine IL-2. We previously showed that SLE T cells express decreased levels of the T-cell receptor-CD3ζ chain and forced expression of CD3ζ into SLE T cells restores IL-2 production. We recently showed that the serine ...

Many mitotic factors were shown to be activated by Ran guanosine triphosphatase. Previous studies in Xenopus laevis egg extracts and in highly proliferative cells showed that mitotic chromosomes were surrounded by steep Ran guanosine triphosphate (GTP) concentration gradients, indicating that RanGTP-activated factors promote spindle assembly around chromosomes. However, the mitotic ...

Despite the biotechnological interest of microalgae, no robust and stable methods for genetic transformation of most microalgal strains exist. The scanty and disperse data about the efficiency of heterologous promoters in microalgae and the use of different transformation methods, DNA quantities and reporter genes in the existing studies makes very ...

We describe temporal changes in the genetic composition of a small anadromous Atlantic salmon (Salmo salar) population from South Newfoundland, an area where salmon populations are considered threatened (COSEWIC 2010). We examined the genetic variability (13 microsatellite loci) in 869 out-migrating smolt and post-spawning kelt samples, collected from 1985 to ...

To identify novel signaling pathways necessary for rhabdomyosarcoma (RMS) survival, we performed a loss-of-function screen using an inducible small hairpin RNA (shRNA) library in an alveolar and an embryonal RMS cell line. This screen identified CRKL expression as necessary for growth of alveolar RMS and embryonal RMS both in vitro ...

Aberrant expression of the oncogenic Kirsten-Ras (Ki-Ras) and interferon-stimulated gene 15 (ISG15) pathways is common in breast and other cancers. However, whether these dysregulated pathways cooperate to promote malignancy is not known. This study links Ki-Ras and ISG15 in a previously unidentified regulatory loop that may underlie malignant transformation of ...

BACKGROUND: As patients with basal-like breast cancer (BLBC) have a poor prognosis and there is no specifically tailored therapy, molecular biological characterization of BLBC is necessary. c-Kit is a transmembrane receptor tyrosine kinase known to play important roles in various solid cancers. This study classified BLBCs from patients with breast ...

To better understand the energetics of accurate DNA replication, we directly measured ∆GO for the incorporation of a nucleotide into elongating dsDNA in solution (∆GOincorporation). Direct measurements of the energetic difference between synthesis of correct and incorrect base pairs found it to be much larger than previously believed (average ∆∆GOincorporation ...

Baseline impedance (BImp) reflects esophageal integrity. The study aims to identify the optimal method to calculate BImp. Mean BImp was calculated in 20 consecutive multichannel intraluminal impedance recordings including and excluding impedance events (IEs) (reflux, swallows, and gas episodes) in all channels during the full recording and during the first ...

ABSTRACT: BACKGROUND: Many genetic diseases are due to defects in protein trafficking where the mutant protein is recognized by the quality control systems, retained in the endoplasmic reticulum (ER), and degraded by the proteasome. In many cases, the mutant protein retains function if it can be trafficked to its proper ...

747 Shigella isolates were collected from hospitals in Jiangsu province of China. Susceptibility to antimicrobials and integron were tested, respectively. 78.3% of S. flexneri and 74.3% S. sonei was resistant to at least three antibiotics. 74.7% Shigella isolates have integron Iand 82.6% of them have integron II. The conjunction between ...

BACKGROUND: Recently, a method of infrared spectroscopy analysis to identify melamine-contained stone was established by examining melamine powders mixed with true urinary stones. However, several studies demonstrated melamine could be interacted with cyanuric acid or uric acid in water through hydrogen bonds. It presents a hypothesis that the infrared spectrum ...

Isobaric peptide termini labeling (IPTL) is based on labeling of both peptide termini with complementary isotopic labels resulting in isobaric peptides. MS/MS analysis after IPTL derivatization produces peptide-specific fragment ions which are distributed throughout the MS/MS spectrum. Thus, several quantification points can be obtained per peptide. In this report, we ...

The human sensor of double-stranded RNA (dsRNA) oligoadenylate synthetase 1 (hOAS1) polymerizes ATP into 2',5'-linked iso-RNA (2-5A) involved in innate immunity, cell cycle, and differentiation. We report the crystal structure of hOAS1 in complex with dsRNA and 2'-deoxy ATP at 2.7 Å resolution, which reveals the mechanism of cytoplasmic dsRNA ...

Ltn1 is a 180-kDa E3 ubiquitin ligase that associates with ribosomes and marks certain aberrant, translationally arrested nascent polypeptide chains for proteasomal degradation. In addition to its evolutionarily conserved large size, Ltn1 is characterized by the presence of a conserved N terminus, HEAT/ARM repeats predicted to comprise the majority of ...

OBJECTIVE: To report a case of blindness due to the ingestion of ivermectin and subsequent successful treatment with intravenous lipid (IVL) therapy. CASE SUMMARY: A female neutered Jack Russell Terrier was examined for acute onset of apparent blindness after being exposed to ivermectin the previous day. The dog appeared to ...

Proper resolution of stalled replication forks is essential for genome stability. Purification of FBH1, a UvrD DNA helicase, identified a physical interaction with replication protein A (RPA), the major cellular single-stranded DNA (ssDNA)-binding protein complex. Compared with control cells, FBH1-depleted cells responded to replication stress with considerably fewer double-strand breaks ...

During early embryonic development, BMP signaling is essential for neural/ non-neural cell fate decisions. BMP signaling inhibits precocious neural differentiation and allows for proper differentiation of mesoderm, endoderm and epidermis. However, the mechanisms underlying the BMP pathway-mediated cell fate decision remain largely unknown. Here, we show that the expression of ...

Three new 'short' metallacycles are selectively obtained from the reaction of a bimetallic Cu(I) 'U-shape' molecular clip with, respectively, the cyano CN(-), tricyanomethanide C(CN)(3)(-), and azido N(3)(-) pseudohalogenide ions. These supramolecular assemblies have been characterized by spectroscopic methods, single-crystal X-ray diffraction, and elemental analysis. The single-crystal X-ray structures of these ...

In an effort to improve survival and reduce late adverse effects of radiation therapy (RT), 25 children <3 years of age with malignant brain tumors received tandem high-dose chemotherapy (HDCT) and auto-SCT following six cycles of induction chemotherapy. RT was either not given or deferred until 3 years of age ...

The phosphatidylinositol 3'-kinase (PI3K) pathway is dysregulated in multiple myeloma (MM); we therefore tested a highly selective class I PI3K inhibitor, GDC-0941, for anti-myeloma activity. Functional and mechanistic studies were first performed in MM cell lines, then extended to primary MM patient samples cultured in vitro. GDC-0941 was then assessed ...

Abstract Objectives: This article reports on a case and reviews some articles on acupuncture- induced pneumothorax published in Chinese and English medical journals since 1980. Case: A 35-year-old man presented with left chest pain and dyspnea after receiving acupuncture. The patient was later diagnosed with a 12% pneumothorax of the ...

International guidelines for the management of nonvariceal upper gastrointestinal bleeding have not been widely adopted in clinical practice. We sought to determine whether a national, multifaceted intervention could improve adherence to guidelines, especially for patients at high risk of nonvariceal upper gastrointestinal bleeding. In this randomized trial, we stratified hospitals ...

Protein search for targets on DNA starts all major biological processes. Although significant experimental and theoretical efforts have been devoted to investigation of these phenomena, mechanisms of protein-DNA interactions during the search remain not fully understood. One of the most surprising observations is known as a speed-selectivity paradox. It suggests ...

Fatty acid synthase (FASN) is a key enzyme in the synthesis of palmitate, the precursor of major nutritional, energetic and signaling lipids. FASN expression is up-regulated in many human cancers and appears to be important for cancer cell survival. Over-expression of FASN has also been found to associate with poor ...

 Due to the widespread use and potential toxicity of organophosphorous pesticides (OPs), multiresidue monitoring of OPs in camellia oil has become increasingly important. A simple, rapid, and effective matrix solid-phase dispersion extraction for the determination of 15 organophosphorous pesticides in camellia oil is described. Related important factors influencing the extraction ...

BackgroundThere is an increasing frequency of requests for cochlear implantation (CI) in deaf children and more detailed image information is necessary for selecting appropriate candidates. Cochlear nerve deficiency (CND) is a contraindication to CI. Magnetic resonance imaging (MRI) has been used to evaluate the integrity of the cochlear nerve. The ...

Protobothrops jerdonii is a common venomous snake that is widely distributed in southwestern China and other adjacent countries of Asia. In this study, the complete mitochondrial genome of P. jerdonii was determined. The circle genome with the 17,239 bp total length contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal ...

Bacterial infections are known to cause severe health threatening conditions like sepsis. All attempts to get the disease under control failed in the past and especially in times of increasing antibiotic resistance, this leads to one of the most urgent medical challenges of our times. We designed a peptide to ...

Membranes composed of multilayer poly(4-styrenesulfonate) (PSS)/protonated poly(allylamine) (PAH) films on porous alumina supports exhibit high monovalent/divalent cation selectivities. Remarkably, the diffusion dialysis K+/Mg2+ selectivity is >350. However, in nanofiltration this selectivity is only 16, suggesting some convective ion transport through film imperfections. Under MgCl2 concentration gradients across either (PSS/PAH)4- or ...

Human speech universally exhibits a 3- to 8-Hz rhythm, corresponding to the rate of syllable production, which is reflected in both the sound envelope and the visual mouth movements. Artificial perturbation of the speech rhythm outside the natural range reduces speech intelligibility, demonstrating a perceptual tuning to this frequency band. ...

Transition metal catalysts direct 1,2-C→C, -O→C and -N→C migrations from β-methylene-β-silyloxy-β-amido-α-diazoacetates with high selectivity. The key to achieve this unique display of differential selectivities relies on steric and stereoelectronic control by their catalytically generated metal carbenes.

Cytochrome c maturation (Ccm) is a post-translational process that occurs after translocation of apocytochromes c to the positive (p) side of energy transducing membranes. Ccm is responsible for the formation of covalent bonds between the thiol groups of two cysteines residues at the heme binding sites of the apocytochromes and ...

Allogeneic hematopoietic stem cell transplantation and solid organ transplantation have become established treatments offered to patients for whom there are otherwise no curative treatment options. Unfortunately, these therapeutic modalities are associated with severe complications that limit its applicability. Alloimmunity is an important cause of morbidity and mortality after both organ ...

OBJECTIVES: Electrical spinal cord stimulators are routinely used in the thoracic spine for back and lower extremity pain. The anatomy of the cervical spine differs significantly from that of the thoracic spine and deserves special considerations if these implants are to be inserted safely in the neck. This paper explores ...

Thalidomide and its analogues (lenalidomide and pomalidomide) are small molecule glutamic acid derivatives of the immunomodulatory drug (IMiD) class. In addition to the immuno-adjuvant and anti-inflammatory properties that define an IMiD, the thalidomide analogues demonstrate an overlapping and diverse range of biological activities, including anti-angiogenic, teratogenic and epigenetic effects. Importantly, ...

OBJECTIVES/HYPOTHESIS: To assess the survival of full-thickness skin grafts and perichondrial cutaneous grafts when placed on subcutaneous soft tissue flaps used in nasal reconstruction. STUDY DESIGN: Retrospective case series from a secondary-care cutaneous cancer practice. METHODS: Twenty-eight patients with nasal defects secondary to basal cell carcinoma excision were included. Clinical ...

In this Perspective we discuss recent trends in the development and applications of fluorescent proteins. We start by providing a historical and structural perspective of spectroscopic and structural aspects, and describe how these properties have made fluorescent proteins essential as 'smart labels' for biosensing and advanced fluorescence imaging. We show ...

The complexity of health care and the recommendation of the Institute of Medicine to increase the proportion of nurses with a baccalaureate degree to 80% by 2020 requires educators and other stakeholders to consider a range of structures for providing RN-to-BSN programs. Although there are relatively few RN-to-BSN programs offered ...

The modelling of tidal turbines and the hydrodynamic effects of tidal power extraction represents a relatively new challenge in the field of computational fluid dynamics. Many different methods of defining flow and boundary conditions have been postulated and examined to determine how accurately they replicate the many parameters associated with ...

Abstract Background: The alarming increase in the prevalence of metabolic syndrome globally demands serious attention. Recently, we reported increased cellular dysfunction of subcutaneous adipose tissue and monocytes contributing to the insulin resistance and increased inflammation in nascent metabolic syndrome. The major circulating leukocyte is the neutrophil (polymorphonuclear neutrophil, PMN). The ...