Search Results
Results 101 - 150 of 2817
1 2 3 4 5 6 7 8 9 10 >
Muthukrishnan Nandhini N Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, - - 2013
Cell-penetrating peptides such as TAT or R9 labeled with small organic fluorophores can lyse endosomes upon light irradiation. The photoendosomolytic activity of these compounds can in turn be used to deliver proteins and nucleic acids to the cytosol of live cells with spatial and temporal control. In this report, we ...
Li Xiang X Laboratory of Chemistry and Cell Biology, New York, New York 10065, - - 2013
Post-translational modifications (PTM) of proteins can control complex and dynamic cellular processes via regulating interactions between key proteins. To understand these regulatory mechanisms, it is critical that we can profile the PTM-dependent protein-protein interactions. However, identifying these interactions can be very difficult using available approaches, as PTMs can be dynamic ...
Griffin H J HJ Discipline of Exercise and Sport Science, Faculty of Health Sciences, The University of Sydney, Sydney, - - 2013
Clinical research on weight management in young women is limited. This randomized controlled trial compared the efficacy of two iso-energetically restricted (5600 kJ) diets [higher protein (HP): 32% protein, 41% carbohydrate, 25% fat or higher carbohydrate (HC): 20, 58, 21%, respectively] in 71 (HP: n = 36; HC: n = 35) young healthy women ...
Hyter Stephen S Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, - - 2013
Keratinocytes contribute to melanocyte activity by influencing their microenvironment, in part, through secretion of paracrine factors. Here, we discovered that p53 directly regulates Edn1 expression in epidermal keratinocytes and controls UV-induced melanocyte homeostasis. Selective ablation of endothelin-1 (EDN1) in murine epidermis (EDN1(ep-/-) ) does not alter melanocyte homeostasis in newborn ...
Barbosa Sónia S Department of Medicine, Imperial College, London, W2 1PG, - - 2013
CREB3 proteins comprise a set of ER-localized bZip transcription factors defined by the presence of a transmembrane domain. They are regulated by inter-compartmental transport, Golgi cleavage and nuclear transport where they promote appropriate transcriptional responses. Although CREB3 proteins play key roles in differentiation, inflammation and metabolism, a general framework relating ...
Bernhards Robert C RC Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0910, - - 2013
The opportunistic pathogen Pseudomonas aeruginosa ranks among the leading causes of nosocomial infection. The type III secretion system (T3SS) aids acute Pseudomonas aeruginosa infection by injecting potent cytotoxins into host cells to suppress the host's innate immune response. Expression of all T3SS-related genes is strictly dependent on the transcription factor ...
Zhang Wei W Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL, - - 2013
Most microorganisms live in complex communities, where they interact both synergistically and competitively. To explore the relationship between environmental heterogeneity and the spatial structure of well-defined biofilms, single- and mixed-species biofilms of Pseudomonas aeruginosa PAO1 and Flavobacterium sp. CDC-65 was grown in a planar flow cell under highly controlled flow ...
Ram Ron R Bone Marrow Transplantation Unit, Rabin Medical Center, Beilinson Hospital and the Sackler School of Medicine, Tel Aviv University, Israel. - - 2013
Abstract Acute graft-versus-host disease (GVHD) has compromised and continues to compromise the benefits associated with allogeneic hematopoietic cell transplant to cure malignant and non-malignant diseases. Pharmacologic interventions to prevent GVHD have emerged as a major objective of research in the immunology and transplant fields. A better understanding of the pathobiology ...
Linden Jennifer R JR Graduate Program in Pathobiology, Brown University, Providence, RI, - - 2013
Candida albicans causes the majority of invasive candidiasis in immunocompromised adults while Candida parapsilosis is a leading cause of neonatal candidiasis. While much work has focused on how the immune system recognizes and responds to C. albicans, less is known about host interaction with C. parapsilosis. This study investigates the human neutrophil ...
Kirk Timothy V - - 2013
Since their introduction in 2001 miniaturized bioreactor systems have made great advances in function and performance. In this article the dissolved oxygen (DO) transfer performance of submilliliter microbioreactors, and 1-10 mL minibioreactors was examined. Microbioreactors have reached k(L) a values of 460 h(-1) , and are offering instrumentation and some ...
Yang Chunyan C National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of - - 2013
The hepatitis E virus (HEV) capsid protein has been demonstrated to be able to assemble into particles in vitro. However, this process and the mechanism of protein-protein interactions during particle assembly remain unclear. In this study, we investigated the assembly mechanism of HEV structural protein subunits, the capsid protein p239 ...
Vinther Tine N TN Diabetes Research Unit, Novo Nordisk A/S, Novo Nordisk Park, Måløv DK-2760, - - 2013
Insulin is a key hormone controlling glucose homeostasis. All known vertebrate insulin analogs have a classical structure with three 100% conserved disulfide bonds that are essential for structural stability and thus the function of insulin. It might be hypothesized that an additional disulfide bond may enhance insulin structural stability which ...
Callahan Brian P BP Department of Biological Sciences, University at Albany, Life Sciences Building 2061, 1400 Washington Avenue, Albany, New York 12222, USA. - - 2013
The unregulated activity of inteins during expression and consequent side reactions during work-up limits their widespread use in biotechnology and chemical biology. Therefore, we exploited a mechanism-based approach to regulate intein autocatalysis for biotechnological application. The system, inspired by our previous structural studies, is based on reversible trapping of the ...
Paillusson Sébastien - - 2013
There is growing evidence supporting a role of extracellular alpha-synuclein in the spreading of Parkinson's disease (PD) pathology. Recent pathological studies have raised the possibility that the enteric nervous system (ENS) is one of the initial sites of alpha-synuclein pathology in PD. We therefore undertook this survey to determine whether ...
Ma Chao C Department of Pharmaceutical Sciences, School of Pharmacy, Computational Chemical Genomics Screening Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, - - 2013
The cannabinoid receptor subtype 2 (CB2) is a promising therapeutic target for blood cancer, pain relief, osteoporosis, and immune system disease. The recent withdrawal of Rimonabant, which targets another closely related cannabinoid receptor (CB1), accentuates the importance of selectivity for the development of CB2 ligands in order to minimize their ...
Graham Joseph G JG Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, - - 2013
The intracellular bacterial pathogen Coxiella burnetii is a category B select agent that causes human Q fever. In vivo, C. burnetii targets alveolar macrophages wherein the pathogen replicates in a lysosome-like parasitophorous vacuole (PV). In vitro, C. burnetii infects a variety of cultured cell lines that have collectively been used to model ...
Zhang Chuanbo C Department of Radiation Medicine, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, - - 2013
TNFAIP8 is a NF-κB-inducible, oncogenic molecule. Previous "promoter array" studies have identified differential methylation and regulation of TNFAIP8 in prostate epithelial and cancer cell lines. Here we demonstrate that TNFAIP8 expression is induced by androgen in hormone-responsive LNCaP prostate cancer cells. In athymic mice bearing hormone-refractory PC-3 prostate tumor xenografts, ...
Jonassaint Naudia L NL Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, - - 2013
Defects in telomere maintenance genes cause pathological telomere shortening, and manifest in syndromes which have prominent phenotypes in tissues of high turnover: the skin and bone marrow. Because the gastrointestinal (GI) epithelium is highly proliferative, we sought to determine whether telomere syndromes cause GI disease, and to define its prevalence, ...
Kim Jae-Hyung - - 2013
BACKGROUND: To measure the efficiency of phacoemulsification, we have developed a new experimental model for testing phaco-efficiency and analyzed re-aspiration of repulsed particles. METHODS: Using a Kitaro wetlab system, a piece of blood agar (BA) was placed in an artificial chamber and the phacoemulsifier was placed horizontally. The settings of ...
Chaudhary M Z - - 2013
Sediment core from Korangi Creek, one of the polluted coastal locations along the Karachi Coast Pakistan, was collected to trace the history of marine pollution and to determine the impact of industrial activity in the area. Down core variation of metals such as Ca, K, Mg, Al, S, Ti, V, ...
Jiang Ling-Feng - - 2013
This study aimed to assess whether maifanite can improve the learning and memory, and antioxidant abilities of Alzheimer's disease (AD) rats. The 70 rats were divided into seven groups: [A] normal control group, [B] AD model group, [C] sham group, [D] positive control group (donepezil), [E] low-dose maifanite group, [F] ...
Froelich Brett - - 2013
The human bacterial pathogen, Vibrio vulnificus, is found in brackish waters and is concentrated by filter-feeding molluscan shellfish, especially oysters, which inhabit those waters. Ingestion of raw or undercooked oysters containing virulent strains of V. vulnificus can result in rapid septicemia and death in 50 % of victims. This review summarizes ...
Wang Run L - - 2013
Two experiments were conducted to examine the effect of zinc (Zn) source on the performance, Zn status, immune response, and rumen fermentation of lactating cows to find the most available Zn source for dairy production. In Experiment 1, a total of 30 multiparous Holstein cows were randomly allocated by body ...
Basu Subhajit - - 2013
A massive algal bloom of the dinoflagellate Noctiluca miliaris (green) was located in the Northern Arabian Sea by IRS-P4-2 (OCM-II) for microbiological studies, during two consecutive cruises of February-March 2009. Culturable bacterial load during bloom were ∼2-3-fold higher in comparison to non-bloom waters and ranged from 3.20 × 10(5) to 6.84 × 10(5) cfu ml(-1). An ...
Rębacz-Maron Ewa - - 2013
Mineral imbalance in the body may significantly contribute to the development and course of hypertension. In this paper, blood pressure figures have been linked to the levels of Fe, Ca, Mg, Zn, Cu, Na and K in hair. The research sample was composed of young men (n = 91) aged 13-21, from ...
Vezzulli Luigi - - 2013
Vibrios are among the most common bacteria that inhabit surface waters throughout the world and are responsible for a number of severe infections both in humans and animals. Several reports recently showed that human Vibrio illnesses are increasing worldwide including fatal acute diarrheal diseases, such as cholera, gastroenteritis, wound infections, ...
Sachdev Dhara P - - 2013
Agricultural productivity to meet growing demands of human population is a matter of great concern for all countries. Use of green compounds to achieve the sustainable agriculture is the present necessity. This review highlights the enormous use of harsh surfactants in agricultural soil and agrochemical industries. Biosurfactants which are reported ...
Segade P - - 2013
SUMMARY Heliciculture is an excellent alternative to obtain edible snails but its viability is seriously threatened by pathogens. A parasitological survey was conducted in 3 mixed system-based heliciculture farms in Galicia (NW Spain), with the species Tetrahymena rostrata, Tetrahymena limacis, Tetratrichomonas limacis, Cryptobia helicogenae, Brachylaima aspersae (metacercariae and sporocysts), Alloionema ...
Cosnier Frédéric - - 2013
Abstract 1. Toluene (TOL) is a neurotoxic, ototoxic and reprotoxic solvent which is metabolized via the glutathione pathway, producing benzylmercapturic, o-, m- and p-toluylmercapturic acids (MAs). These metabolites could be useful as biomarkers of TOL exposure. 2. The aims of this study were (1) to provide data on MAs excretion ...
Gautam Nischal K - - 2013
OBJECTIVES: To study the effect of two protamine-dosing strategies on activated clotting time (ACT) and thromboelastography (TEG). BACKGROUND: Protamine dosage based on neutralizing heparin present in the combined estimated blood volumes (EBVs) of the patient and cardiopulmonary bypass (CPB) pump may result in excess protamine and contributes toward a coagulopathy ...
Liu Jin-Hua - - 2013
Fluorescence anisotropy (FA) is a reliable, sensitive, and robust assay approach for determination of many biological targets. However, it is generally not applicable for assay of small molecules because their molecular masses are relatively too small to produce observable FA value changes. To address this issue, we report herein the ...
Adgate John L - - 2013
Allergens and other pollutants in house dust are collected using a variety of dust samplers that are assumed to operate similarly. This factorial design study compared sampler performance under controlled environmental conditions. House dust with known particle sizes (212-90, 90-45, and <45 μm) and allergen concentrations were sampled from new carpet ...
Malkowska Maja - - 2013
ABSTRACT: Herpesviridae is a large family of DNA viruses divided into three subfamilies: Alpha-, Beta- and Gammaherpesvirinae. The process of herpesvirus transmission is mediated by a range of proteins, one of which is glycoprotein L (gL). Based on our analysis of the solved structures of HSV2 and EBV gH/gL complexes, ...
Lee Chang Hoon - - 2013
Photolysis of [Ni(II)(μ-Cl)Cl(IPr)](2) (IPr = 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene) activates the Ni-Cl bonds to furnish [Ni(I)(μ-Cl)(IPr)](2). Consistent with this photochemistry, a TD-DFT calculation for square planar Ni(ii) centre reveals that light excitation leads to the population of orbitals with σ*(Ni-Cl) character.
Müller Christoph R - - 2013
Enzymes create chiral microenvironments that may simultaneously generate several stereogenic centers in the same catalytic cycle, broadening the possibilities of biocatalysis. Benzaldehyde lyase (BAL) affords highly diastereoselective α-hydroxy-ketones by simultaneously performing ligation and kinetic resolution of a racemic aldehyde. Thus, to the well-known enantioselective BAL-carboligation of aldehydes (C-C bond formation), ...
Kouatli Yasser - - 2013
This case report illustrates the challenges in managing patients with centrifugal ventricular assist devices (VADs). As these devices have evolved, their physical properties have changed, impacting patient management. Levitated centrifugal pumps have a non-linear response to afterloads. The device output will decrease significantly in the presence of high systemic vascular ...
Azarfarin Rasoul - - 2013
BACKGROUND: There is a controversy over using either smaller- or larger-size endotracheal tubes (ETT) in children undergoing cardiac surgery, and some anesthesiologists prefer to use ETT sizes different from the formula-based sizes. The aim of the present study was to compare proper-size cuffed ETT in children undergoing cardiac vs noncardiac ...
Detti Laura - - 2013
Disruption in the normal timing of female puberty, such as in pre-pubertal cancer treatments, can cause abnormal somatic development. We sought to evaluate the impact of cyclophosphamide (CTX) on the somatic, uterine, and ovarian, development of pre-pubertal mice. Pre-pubertal (day 18 of life) C57BL/6J female mice were randomized to receive ...
Dean Rebecca K - - 2013
Lithium compounds of tetradentate amino-bis(phenolato)-tetrahydrofuranyl ligands, Li(2)[] () and Li(2)[] () (where [] = 2-tetrahydrofuranyl-N,N-bis(2-methylene-4-methyl-6-tert-butylphenolate), and [] = 2-tetrahydrofuranyl-N,N-bis(2-methylene-4,6-tert-butylphenolate)) were characterized by multinuclear solution NMR and solid-state (6)Li and (7)Li NMR spectroscopy. The proligands, n-propylamino-N,N-bis(2-methylene-4-methyl-6-tert-butylphenol), (H(2)[]) and benzylamino-N,N-bis(2-methylene-4,6-di-tert-amylphenol), H(2)[] were reacted with n-butyllithium in THF to give the related dilithium ...
Kashiwagi Masayo - - 2013
ABSTRACT: BACKGROUND: In Japan, there is a large increase in the number of elderly persons who potentially need home-visit nursing services (VNS). However, the number of persons using the VNS has increased only little in comparison to the number of individuals who use home social services, which are also covered ...
Zhao Yuting - - 2013
A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe(3)O(4) core was synthesized using cysteamine (SH-NH(2)) as linker, and characterized by TEM, XPS, UV and electrochemistry. Then a hydrolase biosensor, based on self-assembly of methyl parathion hydrolase (MPH) on the Fe(3)O(4)@Au nanocomposite, was developed for sensitive and selective detection of ...
Quintás-Cardama Alfonso - - 2013
Abstract Two important response criteria in systemic mastocytosis (SM) are the elimination or reduction in the percentage of bone marrow mast cells (MCs), and the reduction of serum tryptase levels. We investigated the accuracy of a single time point reduction of bone marrow MCs and serum tryptase level as response ...
Beigy M - - 2013
Increased fructose consumption is linked to insulin resistance, weight gain, hyperlipidemia and hypertension. Although the advantages of several dietary restriction regimens have been demonstrated, the effects of alternate-day fasting (ADF) on fructose-induced insulin resistance have not yet been studied. This study is based on a new modification on ADF by ...
Tse Mimi My - - 2013
AIMS AND OBJECTIVES: To examine the effectiveness of an integrated motivational interviewing and physical exercise programme on pain, physical and psychological function, quality of life, self-efficacy, and compliance with exercise for community-dwelling older persons with chronic pain. BACKGROUND: Chronic pain is common among older persons. Indeed, motivation for managing pain ...
Schaier Matthias - - 2013
Regulatory T cells (Tregs) were shown to be involved into the pathogenesis of acute rejection after transplantation. The suppressive activity of the total regulatory T cell pool depends on its percentage of highly suppressive HLA-DR(+) -Treg cells. Therefore, both the suppressive activity of the total Treg pool and the extent ...
Shabir Shazia - - 2013
Diagnosing new onset diabetes after transplantation (NODAT) by glycated haemoglobin (HbA1c) has not been validated against the gold-standard oral glucose tolerance test (OGTT). We analysed the predictive and optimum value of HbA1c to diagnose NODAT amongst nondiabetic renal transplant recipients. Assessment of glucose metabolism (OGTT and HbA1c) was prospectively undertaken ...
Nomura Tomoe - - 2013
ABSTRACT: BACKGROUND: Aberrant methylation patterns in CpG island are known to be influential in gene silencing. Histamine plays important physiological roles in the upper gastrointestinal tract and acts via the H2 receptor. We report an investigation into the effect of HRH2 promoter polymorphism (rs2607474 G > A) on the methylation ...
Shakhssalim Nasser - - 2013
Purpose: Numerous synthetic materials have been used for the bladder reconstruction; of which, nano-structured scaffolds are used as relevant implant to the bladder tissue-engineering. The aim of this study was to investigate the capacity of Poly ε-caprolactone/poly-L-lactide acid (PCL/PLLA) nanofibrous scaffold, in supporting the maintenance and attachment of the human ...
Iavarone Federica - - 2013
Analysis by HPLC-ESI-MS top-down proteomic platform of specimens of human preterm newborn whole saliva evidenced high relative amounts of cystatin B and its S-glutathionylated, S-cysteinylated and S-S 2-mer (on Cys3) derivatives, decreasing as function of post-conceptional age (PCA). The percentage of S-unmodified cystatin B was higher than the S-modified isoforms ...
Duprez Veerle - - 2013
AIMS AND OBJECTIVES: To develop a set of psychometrically sound instruments to assess knowledge, self-management and self-efficacy of diabetic patients. Furthermore, a survey to evaluate the satisfaction about diabetes education for patients was developed and tested. BACKGROUND: Treatment and secondary prevention of diabetes require a complex combination of care components. ...
1 2 3 4 5 6 7 8 9 10 >