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Binder Nikolaus B - - 2013
To investigate how tumor necrosis factor (TNF)-inhibiting therapy affects bone destruction and inflammation in a TNF-driven mouse model of rheumatoid arthritis. In order to evaluate the influence of TNF on osteoclastogenesis in vitro, different concentrations of TNF were added to spleen cell-derived monocytes in the absence or presence of different ...
Westwood J-P JP Haemostasis Research Unit, University College London, UK. - - 2013
Rituximab has been documented in the treatment of acute (≤ 3 days from admission), relapsed/refractory thrombotic thrombocytopenic purpura (TTP) and given as prophylaxis in selected cases to prevent acute relapse. The precise timing of rituximab in acute TTP has not been determined. To perform a retrospective analysis of rituximab use ...
Mumford A D AD Bristol Heart Institute, University of Bristol, Bristol, UK. - - 2013
Genetic variations that affect the structure of the thromboxane A2 receptor (TP receptor) provide insights into the function of this key platelet and vascular receptor, but are very rare in unselected populations. To determine the functional consequences of the TP receptor Trp29Cys (W29C) substitution. We performed a detailed phenotypic analysis of ...
Nishioka Chie C Department of Immunology, Kochi Medical School, Kochi University, Nankoku, Kochi, - - 2013
Adult T-cell leukaemia-lymphoma (ATLL) is an aggressive malignancy of CD4(+)  CD25(+) T lymphocytes, characterized by a severely compromised immunosystem, in which the human T-cell lymphotropic virus type 1 (HTLV-1) has been recognized as the aetiological agent. This study found that an IκB kinase β (IKKβ) inhibitor Bay11-7082 inactivated mammalian target ...
Keeler B D BD Division of Gastrointestinal Surgery, Queen's Medical Centre, Nottingham, - - 2013
Raised intracellular iron has been identified as a potential aetiological factor in the development of several epithelial malignancies, including those of the gastrointestinal tract. The mechanism behind this increase is thought to include disorders of iron uptake and storage. Several iron chelators have been identified as potential anti-tumour agents, with ...
Wong Eunice C EC RAND Corporation, Santa Monica, CA 90407-2138, USA. - - 2013
This study examined the mental health treatment experiences of active-duty U.S. service members who received treatment from primary care or specialty mental health providers. A national sample of active-duty service members (N=1,659) was surveyed about mental health treatment experiences. About 17% of respondents reported receipt of mental health care in ...
Jeannet Robin R Division of Hematopoietic Stem Cell and Leukemia Research, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, California 91010, - - 2013
Hematopoietic stem cells (HSCs) reside in a specialized bone marrow (BM) microenvironment that supports the maintenance and functional integrity of long-term (LT)-HSCs throughout postnatal life. The objective of this work is to study the role of activated leukocyte cell adhesion molecule (Alcam) in HSC differentiation and self-renewal using an Alcam-null ...
Fisher James E JE Division of Transplant Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, - - 2013
In utero cell transplantation (IUCT) can lead to the postnatal engraftment of human cells in the xenogeneic recipient. Most reports of IUCT have involved hematopoietic stem cells. It is unknown whether human hepatocytes used for IUCT in fetal pigs will lead to the engraftment of these same cells in the ...
Walter Gina J GJ King's College London, London, - - 2013
Despite the high frequency of CD4+ T cells with a regulatory phenotype (CD25+CD127(low) FoxP3+) in the joints of patients with rheumatoid arthritis (RA), inflammation persists. One possible explanation is that human Treg cells are converted into proinflammatory interleukin-17 (IL-17)-producing cells by inflammatory mediators and thereby lose their suppressive function. The ...
Romano Alessandro A Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, - - 2013
Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a neurological disease with mutations in SACS, encoding sacsin, a multidomain protein of 4,579 amino acids. The large size of SACS and its translated protein has hindered biochemical analysis of ARSACS, and how mutant sacsins lead to disease remains largely unknown. Three ...
McGrew John H JH Department of Psychology, Indiana University–Purdue University Indianapolis, Indianapolis, IN 46202, USA. - - 2013
Monitoring fidelity of assertive community treatment (ACT) teams is costly. This study investigated the reliability and validity of a less burdensome approach: self-reported assessment. Phone-administered and self-reported assessments were compared for 16 ACT teams. Team leaders completed a self-report protocol providing information sufficient to score the Dartmouth Assertive Community Treatment ...
Jansson S P O SP Family Medicine Research Centre, Örebro County Council, School of Health and Medical Sciences, Örebro University, Sweden. - - 2013
To analyse the effects of hyperglycaemia and blood pressure, diabetes and anti-hypertension treatment on total and various types of cardiovascular disease incidence in patients with Type 2 diabetes followed for 30 years. A total of 740 incident patients with Type 2 diabetes were registered at the Laxå Primary Health Care Centre, ...
Røgen Peter P Department of Mathematics, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark. - - 2013
Protein structure prediction techniques proceed in two steps, namely the generation of many structural models for the protein of interest, followed by an evaluation of all these models to identify those that are native-like. In theory, the second step is easy, as native structures correspond to minima of their free ...
Tryon Robert C RC Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA. - - 2013
The regulation of transcription and translation by specific cell types is essential to generate the cellular diversity that typifies complex multicellular organisms. Tagging and purification of ribosomal proteins has been shown to be an innovative and effective means of characterizing the ribosome bound transcriptome of highly specific cell populations in ...
Park Jung-Ho JH Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon-gun, Chungcheongbuk-do, Republic of - - 2013
MazF is an mRNA interferase that cleaves mRNAs at a specific RNA sequence. MazF from E. coli (MazF-ec) cleaves RNA at A^CA. To date, a large number of MazF homologs that cleave RNA at specific three- to seven-base sequences have been identified from bacteria to archaea. MazF-ec forms a dimer, ...
Taylor Kyle R KR Department of Electrical and Computer Engineering, University of Iowa, Iowa City, IA, - - 2013
Autosomal dominant nonsyndromic hearing loss (ADNSHL) is a common and often progressive sensory deficit. ADNSHL displays a high degree of genetic heterogeneity and varying rates of progression. Accurate, comprehensive, and cost-effective genetic testing facilitates genetic counseling and provides valuable prognostic information to affected individuals. In this article, we describe the ...
Grimes Vaughan - - 2013
The integrity of the biological phosphate oxygen isotope (δ(18) O(p) ) signal is thought to be contingent upon the complete removal of competing sources of oxygen such as associated organic matter. A range of pretreatment methods to purify phosphate material from competing sources of oxygen has been reported, with contradictory ...
Gilbert-López Bienvenida - - 2013
The present study reports on the evaluation of dielectric barrier discharge microplasma ionization (DBDI) for liquid chromatography/high resolution mass spectrometry (LC/HRMS) analyses of pesticide residues in fruit and vegetables. Ionization, fragmentation, analytical performance and matrix effects displayed by LC/DBDI-MS were critically evaluated and compared with both atmospheric pressure chemical ionization ...
Feakins Sarah J - - 2013
A published method to determine the hydrogen isotope ((2) H/(1) H) ratios of methoxyl groups, cleaved from lignin by hydroiodic acid (HI), calls for the analysis of methyl iodide (CH(3) I). However, analysis of halogenated compounds by gas chromatography/pyrolysis/isotope ratio mass spectrometry (GC/P/IRMS) yields incomplete conversion into H(2) and unwanted ...
Martínez-Villalba Anna - - 2013
Direct analysis in real time (DART) is a novel ionization technique that has been demonstrated in numerous applications as a useful tool for fast and convenient mass spectrometry (MS)-based analysis of complex samples. In this study, the feasibility of DART ionization coupled to a high-resolution mass spectrometer utilizing an orbitrap ...
Ding Xiao - - 2013
Drug discovery samples are routinely analyzed using liquid chromatography/tandem mass spectrometry (LC/MS/MS) methods on triple quadrupole mass spectrometers employing multiple reaction monitoring (MRM). In order to improve analysis throughput, quantitation of small molecules on a quadrupole time-of-flight (QqTOF) instrument using TOF scan and high-resolution MRM (MRM-HR) modes was evaluated in ...
Olejnik Małgorzata - - 2013
During the development and validation of mass spectrometry based method in residue control analysis, it is recommended to evaluate the level of the matrix effect. Often its level is relatively high, despite extensive sample purification. The matrix effect in the method for the determination of non-steroidal anti-inflammatory drugs in animal ...
Kornilova Tatiana A - - 2013
A principal limitation of gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) is the thermal instability of analytes. We propose that the injector and column temperatures should not exceed the atmospheric pressure boiling point, without decomposition, of the highest homologue of the series being analyzed, instead of the time-consuming procedure ...
Bienvenut Willy V - - 2013
Some large-scale proteomics studies in which strong cation exchange chromatography has been applied are used to determine proteomes and post-translational modification dynamics. Although such datasets favour the characterisation of thousands of modified peptides, e.g., phosphorylated and N-α-acetylated, a large fraction of the acquired spectra remain unexplained by standard proteomics approaches. ...
Karamonová Ludmila - - 2013
The bacterial genus Cronobacter was established quite recently, in 2008. Therefore, its systematic classification is still in progress as well as the risk assessment of Cronobacter strains. The possibility of rapid identification within the biogroup level has an essential epidemiological significance. We examined the potential of mass spectrometry to accomplish ...
Lobas Anna A - - 2013
High-throughput methods of proteomics are essential for identification of proteins in a cell or tissue under certain conditions. Most of these methods require tandem mass spectrometry (MS/MS). A multidimensional approach including predictive chromatography and partial chemical degradation could be a valuable alternative and/or addition to MS/MS. In the proposed strategy ...
Ablajan Keyume - - 2013
Flavonoids exhibit a wide range of biological activities. The differentiation and structural characterization of the flavonol O-rhamnosides and their isomers are important research topics. Electrospray ionization tandem mass spectrometry (ESI-MS(n) ) is an indispensable tool and has become an efficient method for the identification of bioactive natural products. Negative ion ...
Zachariadis George A - - 2013
The capabilities of modified non-spherical Doehlert-type experimental designs to optimize the performance of a hybrid mass spectrometer were investigated in this paper for the first time. The optimization process was completed in three successive steps with groups of variables avoiding any univariable approach. Glutathione (GSH, reduced) was selected as a ...
Puccio Giovanni - - 2013
Thiodiglycolic acid (TDGA) is a urinary metabolite of the oxazaphosphorine class of chemotherapeutics, in particular of ifosfamide. Ifosfamide metabolism generates chloroacetaldehyde (CAA), a toxic compound associated with neurotoxicity, nephrotoxicity, urotoxicity and cardiotoxicity. CAA, in turn, interacts with cellular thiol groups leading to GSH depletion, cell death and generation of thiodiglycolic ...
Zhao Yue - - 2013
Nucleotide analogs are highly polar and ionic, which impose great challenges on bioanalysis. Ion-pairing liquid chromatography/tandem mass spectrometry (LC/MS/MS) is the predominant reported approach for such compounds. Assay ruggedness of ion-pairing LC/MS/MS methods was often a challenge due to the potential contamination of the ion source of the mass spectrometer ...
Chang Cynthia X L - - 2013
Conditional ligands have enabled the high-throughput production of human leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with European ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel irradiation-sensitive ligands, specifically targeting South East Asian populations, which ...
Hussain Shehnaz K SK Department of Epidemiology, Fielding School of Public Health, University of California, Los Angeles, CA, USA. - - 2013
Given the important role of cell mediated immunity in viral clearance and control of premalignant lesions, we hypothesize that variation in the IL-12/IL-10 cytokine and cytokine receptor genes may influence cervical and vulvar cancer risk. We evaluated 76 tagSNPs from seven candidate genes (IL-10, IL-12A, IL-12B, IL-10RA, IL-10RB, IL-12RB1, and ...
Basu Roy Upal K UK Department of Biochemistry and Molecular Biophysics, Biochemistry and Molecular and Cellular Biology Graduate Program, University of Arizona, Tucson, AZ, - - 2013
Caveolin-1 is an essential component of membrane caveolae. It is an important regulator of cellular processes such as signal transduction and endocytosis. We report here, for the first time, that caveolin-1 is a target of the K-RAS oncogene in colon carcinogenesis. Caveolin-1 is induced in colon cancer cells and in ...
North Ashley A Department of Physiology, Meharry Medical College, Nashville, TN 37208, - - 2013
Methamphetamine (METH) is a highly addictive and neurotoxic psychostimulant. Its use in humans is often associated with neurocognitive impairment. Whether this is due to long-term deficits in short-term memory and/or hippocampal plasticity remains unclear. Recently, we reported that METH increases baseline synaptic transmission and reduces LTP in an ex vivo ...
Patani Neill N The Breakthrough Breast Cancer Research Center, The Institute of Cancer Research, London, United - - 2013
The higher incidence of breast cancer in developed countries has been tempered by reductions in mortality, largely attributable to mammographic screening programmes and advances in adjuvant therapy. Optimal systemic management requires consideration of clinical, pathological and biological parameters. Oestrogen receptor alpha (ERα), progesterone receptor (PgR) and human epidermal growth factor ...
Masuda Melisa M Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, - - 2013
Chronic elevated exposure to manganese (Mn) is associated with neurocognitive and fine motor deficits in children. However, relatively little is understood about cellular responses to Mn spanning the transition between physiologic to toxic levels of exposure. Here, we investigated the specificity, sensitivity, and time course of the Golgi Phosphoprotein 4 ...
Gray Michael J MJ Women's Cancer Program, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, - - 2013
Overexpression of the unfolded protein response master regulator GRP78 is associated with poor prognosis and therapeutic resistance in numerous human cancers, yet its role in endometrial cancers (EC) is undefined. To better understand the contribution of GRP78 to EC, we examined its expression levels in EC patient samples and EC ...
Je Eun Mi - - 2013
Recurrent somatic mutations in splicing machinery components, including SF3B1, U2AF1 and SRSF2 genes have recently been reported in myelodysplastic syndromes (MDS). Such a recurrent nature strongly suggests that these mutations play important roles in tumor development. To see whether SF3B1, U2AF1 and SRSF2 mutations occur in other human tumors besides ...
González Carlos A - - 2013
The identification and surveillance of patients with preneoplastic lesions at high risk of progressing to gastric cancer (GC) represents the most effective way of reducing the burden of GC. The incomplete type of intestinal metaplasia (IM) could be considered as the best candidate for surveillance. However, the usefulness of subtyping ...
Ohanian Maro M Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, - - 2013
Extramedullary manifestations of acute myeloid leukemia (AML) were described as early as the 19th century. However, the incidence, clinical significance and pathobiology of extramedullary AML remain ill defined. We reviewed case reports, retrospective case series, pilot studies and imaging studies of extramedullary leukemia (EML) to determine its frequency, characteristics, clinical ...
Kuruppu Sanjaya S Department of Biochemistry & Molecular Biology, Monash University, Wellington Rd, Clayton, Victoria 3800, Australia. - - 2013
Endothelin-1 (ET-1) is one of the most potent peptide vasoconstrictors known. It is produced upon the cleavage of its precursor big endothelin-1 by endothelin converting enzyme-1 (ECE-1). Production of ET-1 is thought to be dependent upon the expression of ECE-1 at the cell surface. Therefore, mechanisms inducing the trafficking of ...
Sanderson Joseph P JP Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, Sir Henry Wellcome and Hope Laboratories, Southampton Musculoskeletal BRU, University of Southampton, Southampton, United - - 2013
Isoglobotrihexosylceramide (iGb3) has been identified as a potent CD1d-presented self-antigen for mouse invariant natural killer T (iNKT) cells. The role of iGb3 in humans remains unresolved, however, as there have been conflicting reports about iGb3-dependent human iNKT-cell activation, and humans lack iGb3 synthase, a key enzyme for iGb3 synthesis. Given ...
Dannemiller K C KC Department of Chemical and Environmental Engineering, Yale University, New Haven, CT, - - 2013
Formaldehyde is a colorless, pungent gas commonly found in homes and is a respiratory irritant, sensitizer, carcinogen, and asthma trigger. Typical household sources include plywood and particleboard, cleaners, cosmetics, pesticides, and others. Development of a fast and simple measurement technique could facilitate continued research on this important chemical. The goal ...
Starr Marlene E ME Department of Surgery, University of Kentucky, Lexington, KY 40536-0298, - - 2013
Tolerance to physiological stress resulting from inflammatory disease decreases significantly with age. High mortality rates, increased cytokine production, and pronounced thrombosis are characteristic complications of aged mice with acute systemic inflammation induced by injection with lipopolysaccharide (LPS). As adipose tissue is now recognized as an important source of cytokines, we ...
Alessi Sheila M SM Calhoun Cardiology Center, University of Connecticut Health Center, Farmington, CT, - - 2013
Contingency management (CM) uses tangible incentives to systematically reinforce abstinence and is among the most efficacious psychosocial substance abuse treatments. This study assessed the feasibility and initial efficacy of a portable CM procedure designed to address technical limitations for detecting drinking that have prevented using CM for alcohol problems. Participants ...
Spulber G G Institute of Clinical Medicine, Unit of Neurology, University of Eastern Finland, University Hospital of Kuopio, Kuopio, Finland. - - 2013
Structural magnetic resonance imaging (MRI) is sensitive to neurodegeneration and can be used to estimate the risk of converting to Alzheimer's disease (AD) in individuals with mild cognitive impairment (MCI). Brain changes in AD and prodromal AD involve a pattern of widespread atrophy. The use of multivariate analysis algorithms could ...
Muthukrishnan Nandhini N Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, - - 2013
Cell-penetrating peptides such as TAT or R9 labeled with small organic fluorophores can lyse endosomes upon light irradiation. The photoendosomolytic activity of these compounds can in turn be used to deliver proteins and nucleic acids to the cytosol of live cells with spatial and temporal control. In this report, we ...
Li Xiang X Laboratory of Chemistry and Cell Biology, New York, New York 10065, - - 2013
Post-translational modifications (PTM) of proteins can control complex and dynamic cellular processes via regulating interactions between key proteins. To understand these regulatory mechanisms, it is critical that we can profile the PTM-dependent protein-protein interactions. However, identifying these interactions can be very difficult using available approaches, as PTMs can be dynamic ...
Griffin H J HJ Discipline of Exercise and Sport Science, Faculty of Health Sciences, The University of Sydney, Sydney, - - 2013
Clinical research on weight management in young women is limited. This randomized controlled trial compared the efficacy of two iso-energetically restricted (5600 kJ) diets [higher protein (HP): 32% protein, 41% carbohydrate, 25% fat or higher carbohydrate (HC): 20, 58, 21%, respectively] in 71 (HP: n = 36; HC: n = 35) young healthy women ...
Hyter Stephen S Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, - - 2013
Keratinocytes contribute to melanocyte activity by influencing their microenvironment, in part, through secretion of paracrine factors. Here, we discovered that p53 directly regulates Edn1 expression in epidermal keratinocytes and controls UV-induced melanocyte homeostasis. Selective ablation of endothelin-1 (EDN1) in murine epidermis (EDN1(ep-/-) ) does not alter melanocyte homeostasis in newborn ...
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