Search Results
Results 101 - 150 of 2028
1 2 3 4 5 6 7 8 9 10 >
Gadepalli Venkat S - - 2013
Cancer stem cells possess the ability to self-renew and differentiate into specific cells found in tumor types, a characteristic feature of normal multipotent stem cells. These cells harbor within the bulk of tumors and if the tumor suppressor p53 is mutated in these cells, can be more likely to cause ...
Doonan Francesca - - 2013
Terminal dUTP nick end labeling (TUNEL) is an invaluable technique used in the study of late-stage apoptosis. The technique is based upon detection of fragmented DNA, a well-recognized characteristic of apoptosis, usually with fluorescent markers. Here, we describe the TUNEL technique (1) employing two different detection techniques, fluorescence microscopy and ...
Barrero Maria J - - 2013
RNA polymerase II (Pol II) is the main engine that drives transcription of protein-encoding genes in eukaryotes. Despite its intrinsic subunit complexity, Pol II is subject to a host of factors that regulate the multistep transcription process. Indeed, the hallmark of the transcription cycle is the dynamic association of Pol ...
Vaseva Angelina V - - 2013
p53 is a master regulator of cell death pathways and has transcription-dependent and transcription-independent modes of action. Mitochondria are major signal transducers in apoptosis and are critical for p53-dependent cell death. Our lab and others have discovered that a fraction of stress-induced wild-type p53 protein rapidly translocates to mitochondria upon ...
Strauß Olaf - - 2013
Ca(2+) is an important regulator of many cell functions including proliferation, apoptosis, movements, secretion, contraction, excitation, and differentiation. The regulation of these different cell functions is encoded by the specific temporal and spatial distribution of Ca(2+) signals. In degenerative diseases mutations can lead to changes in cell functions in the ...
Marques Sueli - - 2013
Neurodegenerative disorders, such as Parkinson's and Alzheimer's disease, are highly complex, due to their multifactorial origin, not only depending on genetic but also on environmental factors. Several genetic risk factors have already been associated with both the diseases, however, the precise way through which the environment contributes to neurodegeneration is ...
Portier Emilie - - 2013
Biofilm formation could be studied in various conditions. Most of the studies with Legionella pneumophila used monospecies biofilm in culture media. In some cases, it is important to study bacteria in conditions more close to environmental conditions. In this paper, we describe protocols to produce natural biofilms from river water ...
Dhamija Neeru - - 2013
Despite the intense effort put by researchers globally to understand Human Immunodeficiency Virus (HIV-1) pathogenesis since its discovery 30 years ago, the acquired knowledge till date is not good enough to eradicate HIV-1 from an infected individual. HIV-1 infects cells of the human immune system and integrates into the host ...
Carter Debra A - - 2013
The identification of stem/progenitor cells within the retinal neural environment has opened up the possibility of therapy via cellular replacement and/or reprogramming of resident cell populations (1-4). Within the neuro-retinal niche, following injury or in disease states (including inflammation and degeneration), cellular responses affect tissue homeostasis, reduce cell density, disrupt ...
Meikar Oliver - - 2013
Male germ cell differentiation is a complex developmental program that produces highly specialized mature spermatozoa capable of independent movement and fertilization of an egg. Germ cells are unique in their capability to generate new organisms, and extra caution has to be taken to secure the correct inheritance of genetic and ...
Mueller Niklaus H - - 2013
The prevalence of cataract increases with age. Conversely, the abundance of native α-crystallin diminishes with age and cataract development. We hypothesize replenishing lens α-crystallin may delay or prevent cataract. Herein we investigated the ability of cell penetration peptides (CPP) to enhance entry of α-crystallins into lens-derived cells. Recombinant αB-crystallins were ...
Zhang Qi - - 2013
Fluorescence polarization (FP) has become a powerful technique to quantitatively analyze the binding of a small soluble fluorescence-labeled probe to a larger soluble protein and its displacement by other molecules. Here, we describe a detailed protocol to identify small molecules capable of targeting p53-MDM2/MDMX interactions using a fluorescence polarization assay ...
De Jesús Dennise A DA Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, - - 2013
RNA interference (RNAi) is the process of specific gene silencing by the use of double-stranded RNA (dsRNA). In cultured Drosophila cells, RNAi methodologies are well established and easily executed: dsRNA, when added to the cell culture medium, is efficiently internalized by the cells and, through the activity of endogenous processing ...
Chang Bo B The Jackson Laboratory, Bar Harbor, ME, USA, - - 2013
Mouse models, with their well-developed genetics and similarity to human physiology and anatomy, serve as powerful tools with which to investigate the etiology of human retinal degeneration. Mutant mice also provide reproducible, experimental systems for elucidating pathways of normal development and function. Here, I describe the tools used in the ...
Zhu Wenhan W Department of Biological Sciences, Purdue University, West Lafayette, IN, - - 2013
Legionella pneumophila the causative agent of Legionnaires' disease, actively manipulates host cell -processes to establish a membrane-bound replication vacuole permissive for its replication. Establishment of such replication niche requires the Dot/Icm type IV secretion system which translocates a plethora of effectors into host cells. Determining whether a particular protein is ...
Jadhav Shivaji K SK National Institute for Research in Reproductive Health [NIRRH], Indian Council of Medical Research [ICMR], Parel, Mumbai, - - 2013
HIV binds specifically to the human mannose receptor (hMR) on vaginal epithelial cells that are devoid of a conventional CD4 receptor. HIV binding to hMR on vaginal epithelial cells induces the production of matrix metalloproteinase 9 (MMP9) leading to degradation of the extracellular matrix, which may increase the risk of ...
Sethna Saumil S Department of Biological Sciences, Fordham University, Bronx, NY, - - 2013
Counting rhodopsin-positive phagosomes residing in the retinal pigment epithelium (RPE) in the eye at different times of day allows a quantitative assessment of engulfment and digestion phases of diurnal RPE phagocytosis, which efficiently clears shed photoreceptor outer segment fragments (POS) from the neural retina. Comparing such activities among age- and ...
Mao Yingyu Y Department of Biological Sciences, Fordham University, Bronx, NY, - - 2013
Retinal pigment epithelial (RPE) cells are among the most actively phagocytic cells in nature. Primary RPE and stable RPE cell lines provide experimental model systems that possess the same phagocytic machinery as RPE in situ. Upon experimental challenge with isolated photoreceptor outer segment fragments (POS), these cells promptly and efficiently ...
Blackshaw Seth S The Salomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD, USA. - - 2013
The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we ...
Vogrin Adam J AJ Department of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, - - 2013
The translocation of effector proteins by the Dot/Icm type IV secretion system is central to the ability of Legionella pneumophila to persist and replicate within eukaryotic cells. The subcellular localization of translocated Dot/Icm proteins in host cells provides insight into their function. Through co-staining with host cell markers, effector proteins ...
Wei Zhiyun Z Department of Medicine, Institute for Genomic Medicine, University of California at San Diego, VA San Diego Healthcare System, La Jolla, California 92093-0838, - - 2013
The neuropeptide Y(2) G-protein-coupled receptor (NPY2R) relays signals from PYY or neuropeptide Y toward satiety and control of body mass. Targeted ablation of the NPY2R locus in mice yields obesity, and studies of NPY2R promoter genetic variation in more than 10,000 human participants indicate its involvement in control of obesity ...
Cheng Christiana L CL Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, - - 2013
Identification and visualization of specific cells and cellular structures in the retina are fundamental for understanding the visual process, retinal development, disease progression, and therapeutic intervention. The increased usage of transgenic and naturally occurring mutant mice has further emphasized the need for retinal cell-specific imaging. Immunofluorescence microscopy of retinal cryosections ...
Berrington William R WR Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington School of Medicine, Seattle, WA, - - 2013
Legionella pneumophila is a facultative intracellular pathogen that is an important cause of pneumonia. Although host factors that may predispose to acquisition of Legionnaire's Disease (LD) include comorbid illnesses (e.g., diabetes, chronic lung disease), age, male sex, and smoking, many individuals have no identifiable risk factors. Some studies suggest that ...
Damjanovic Ana A Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, - - 2013
Most mutations at position 15 in the N-terminal arm of the regulatory protein AraC leave the protein incapable of responding to arabinose and inducing the proteins required for arabinose catabolism. Mutations at other positions of the arm do not have this behavior. Simple energetic analysis of the interactions between the ...
Dailly Eric E Clinical Pharmacology Department, CHU de Nantes, Nantes, France. - - 2013
Abstract The influence of efavirenz, etravirine, raltegravir, and nevirapine administration on the pharmacokinetics of ritonavir-boosted darunavir was investigated using population pharmacokinetics analysis. The population was composed of 142 patients infected with HIV: darunavir plus nucleoside/nucleotide reverse transcriptase inhibitors (NRTI), 54 patients (group A); darunavir plus efavirenz±NRTI, 4 patients (group B); ...
Gobbato Luca - - 2012
PURPOSE:: To compare early bone healing around different experimental titanium implant surfaces and to evaluate the role of a calcium phosphate-coated implant surface because it relates to bone-implant contact (BIC). METHODS:: An experimental hydroxyapatite (HA) grit-blasted and dual acid-etched titanium surface (BAE-1) was compared to an experimental HA grit-blasted and ...
Christian W Jay - - 2012
Several studies have demonstrated relationships between neighborhood-level retail food environments and obesity, race/ethnicity, and socioeconomic status. Most, however, have been limited by the use of residential neighborhoods to define food environments. This study recruited 121 participants to supply three days of Global Positioning System (GPS) tracking data to explore daily ...
Green Peter J - - 2012
Background:Hypertrophic lupus is an uncommon clinical variant of chronic cutaneous lupus that remains a challenge to treat. A 45-year-old female day-care worker with long-standing lupus presented with hypertrophic cutaneous involvement on the dorsal hand, elbow, and toe of 6 years' duration. Treatmentsincluded monotherapy with either hydroxychloroquine or chloroquine and potent ...
Patteet Lisbeth - - 2012
ABSTRACT:: The aim of this review is to provide information for interpreting outcome results from monitoring of antipsychotics in biological samples. A brief overview of the working mechanisms, pharmacological effects, drug interactions, and analytical methods of classical and atypical antipsychotics is given. Nineteen antipsychotics were selected based on their importance ...
Levy Bruce A - - 2012
Combined posterior cruciate ligament, anterior cruciate ligament, and lateral-side disruption is one of the more common patterns of multiligament knee injury. This is a devastating injury with significant long-term functional sequelae, making accurate diagnosis and management extremely important. While surgical intervention is necessary to restore function, the specific management strategies ...
Vender Ron - - 2012
Background:Etanercept is well tolerated and effective in moderate to severe psoriasis; however, data on patient-reported outcomes (PROs) in Canadian patients remain limited.Objective:To assess PROs in Canadian patients with moderate to severe psoriasis receiving etanercept in an open-label trial more representative of general clinical practice than traditional research studies.Methods:This 1-year, multicenter, ...
Dwyer Tim - - 2012
The management of multiligament knee injury mandates a comprehensive understanding of the anatomy on all sides of the joint. Imperative to repair or reconstructive procedures is an intimate knowledge of the structure of the cruciate and collateral ligaments, as well as the complex confluence of structures that comprise the posteromedial ...
Hart Nathan D - - 2012
Quadriceps rupture off the patella is traditionally repaired by a transosseous tunnel technique, although a single-row suture anchor repair has recently been described. This study biomechanically tested a new transosseous equivalent (TE) double-row suture anchor technique compared with the transosseous repair for quadriceps repair. After simulated quadriceps-patella avulsion in 10 ...
Nageris Ben I - - 2012
HYPOTHESIS: In the absence of patent cochlear windows, cochlear fluid inertia depends on the presence of a "third window" as a major component of the bone-conduction response. BACKGROUND: Studies have shown conflicting results regarding changes in air and bone conduction whenever, the round window, oval window, or both windows were ...
Tolk Jaap J - - 2012
In total knee arthroplasty (TKA), reconstruction of a neutral mechanical axis is of great importance. The main goal of this study was to compare the accuracy of alignment after conventional versus computer-assisted TKA. Additionally, the effect of computer-assisted surgery (CAS) on functional outcome was analyzed. Out of a consecutive series, ...
Zinzani P L - - 2012
Treatment regimens of patients with cutaneous T-cell lymphoma (CTCL) vary widely based on clinician preference and patient tolerance. Skin directed therapies are recommended for patients with early stage IA and IB MF, with combinations used in refractory cases. While no regimen has been proven to prolong survival in advanced stages, ...
Setlur Jennifer - - 2012
PURPOSE OF REVIEW: Historically, information gained from the treatment of unilateral true vocal cord paralysis (UVCP) in adults was the same used to treat children. Today, there is a growing body of literature aimed specifically at the treatment of this condition in children. It is an area of growing interest ...
Brainard Laura - - 2012
OBJECTIVE: To determine the incidence of intracranial hypertension in patients with spontaneous encephalocele with cerebrospinal fluid (CSF) leak. STUDY DESIGN: Retrospective case review. SETTING: Tertiary care neurotology practice. PATIENTS: Patients presenting between 2008 and 2011 with spontaneous encephalocele and CSF leak in the temporal bone. INTERVENTION(S): Lumbar puncture with opening ...
Rubegni P - - 2012
AIM:With the progressive aging of the Italian population, geriatric health care has become a major issue for health authorities. However, little data is available regarding geriatric skin diseases. In order to provide rapid access to specialist help, in 2003 we created a dermatology clinic dedicated only to geriatric patients age ...
Girolomoni G - - 2012
Calcipotriol, a vitamin D analogue, and betamethasone dipropionate, a high potency corticosteroid, are complementary agents for the topical treatment of psoriasis vulgaris. Robust evidence on the efficacy and safety of their fixed combination has been provided by randomized, double-blind, controlled clinical trials involving more than 7000 patients with the ointment ...
Pallas V - - 2012
ABSTRACT Prunus spp. are affected by a large number of viruses, causing significant economic losses through either direct or indirect damage, which results in reduced yield and fruit quality. Among these viruses, members of the genus Ilarvirus (isometric labile ringspot viruses) occupy a significant position due to their distribution worldwide. ...
Hou-Dong Li - - 2012
Background:The origin of wound-healing fibroblasts is still debated. Dermal papilla cells (DPCs), which are an important population of stem cells for the regeneration of hair follicles, play a considerable role in cutaneous wound healing. Based on the plasticity of DPCs in wound healing, we hypothesized that DPCs may contribute to ...
Ro Han - - 2012
BACKGROUND:: Wide variation in tacrolimus concentrations and low tacrolimus exposure have been reported to be associated with poor renal graft outcomes in non-Asians. The CYP3A5 polymorphism is a representative genetic factor that might affect this association, together with environmental factors. We investigated whether tacrolimus variability or the mean tacrolimus trough ...
Paulli M - - 2012
Primary cutaneous B-cell lymphoma (PCBCL) is an heterogeneous group of lymphoproliferative disorders, which account for 25-30% of all primary cutaneous lymphoma and include three main histotypes: 1) primary cutaneous marginal zone B-cell lymphoma (PCMZL); 2) primary cutaneous follicular center cell lymphoma (PCFCL); 3) primary cutaneous diffuse large B-cell lymphoma (DLBCL), ...
Dowling Patrick T - - 2012
After identifying many unlicensed Hispanic international medical graduates (IMGs) legally residing in southern California, University of California, Los Angeles developed an innovative program to prepare these sidelined physicians to enter family medicine residency programs and become licensed physicians. On completion of a 3-year family medicine residency-training program, these IMGs have ...
Stannard James P - - 2012
Dislocations resulting in multiligament knee injuries are challenging to treat and diagnose. With proper diagnosis and anatomic reconstruction techniques, patients can have successful outcomes. This article describes the senior author's (J.P.S.'s) preferred reconstruction techniques, timing for surgery, and rehabilitation techniques for injuries involving the anterior cruciate ligament (ACL), posterior cruciate ...
Higuchi Fujio - - 2012
Background:Ceramics are inorganic nonmetallic materials and are used as bioinert components in joint replacement surgeries. Ceramics are known to be low allergenic. We experienced a ceramic-induced psoriasis.Objective:We report a first case of possible ceramic-induced psoriasis caused by a ceramic insert.Methods:A 55-year-old female received an implanted ceramic-on-ceramic total hip replacement for ...
Osborne Anwar Dayan - - 2012
BACKGROUND:: The American College of Cardiology/American Heart Association guidelines for ST-elevation myocardial infarction state that an electrocardiogram (ECG) should be performed on patients with suspected acute coronary syndrome upon presentation to the emergency department (ED) within 10 minutes. OBJECTIVE:: To determine how previously published clinical criteria for obtaining an ECG ...
Laberge Lauren - - 2012
Background:The Ministry of Health and Long-Term Care, in coordination with Cancer Care Ontario, records and analyzes wait times for cancer surgery in the province of Ontario. However, this strategy does not include wait times for skin cancer surgery.Purpose:The wait times and referral patterns of patients undergoing Mohs micrographic surgery at ...
Veraldi S - - 2012
We describe two cases of hookworm-related cutaneous larva migrans acquired in Brittany (North-Western France). The patients were a 23-year-old woman and a 28-year-old man. In both patients the feet were involved. In the second patient, a superinfection due to Staphylococcus aureus was recorded. The appearance in Brittany of a reservoir ...
1 2 3 4 5 6 7 8 9 10 >