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Nielsen Jens J Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, Sweden. - - 2013
Production of recombinant proteins for use as pharmaceuticals, so-called biopharmaceuticals, is a multi-billion dollar industry. Many different cell factories are used for the production of biopharmaceuticals, but the yeast Saccharomyces cerevisiae is an important cell factory as it is used for production of several large volume products. Insulin and insulin ...
Gokmen Zeynel - - 2012
Objective To evaluate the diagnostic potential of resistin in sepsis and to compare results with C-reactive protein (CRP) in infants < 32 weeks of gestation.Study Design A total of 64 infants were prospectively included in the study. Blood samples were collected for basal CRP and resistin within the first hour of life. When sepsis ...
Zervosen Astrid - - 2013
Although the rate of acylation of a penicillin-resistant form of Streptococcus pneumoniae penicillin-binding protein 2x (PBP2x) by ceftaroline is 80-fold lower than that of its penicillin-sensitive counterpart, it remains sufficiently high (k(2)/K = 12,600 M(-1) s(-1)) to explain the sensitivity of the penicillin-resistant strain to this new cephalosporin. Surprisingly, the ...
Park Kyung Man - - 2012
Even though matrix-assisted laser desorption ionization (MALDI) is a powerful technique for mass spectrometry of peptides and proteins, it is not quite useful for their quantification that is one of the outstanding problems in quantitative proteomics. The main difficulty lies in the poor reproducibility of MALDI spectra. In this work, ...
Wu Yi-Xin - - 2012
Baicalin and scutellarin are the major active principal flavonoids extracted from the Chinese herbal medicines Scutellaria baicalensis and Erigeron breviscapus (Vant.) Hand-Mazz. It has recently been reported that baicalin and scutellarin have antitumor activity. However, the mechanisms of action are unknown. We previously reported that some flavonoids have a specific ...
Manno Carlo C Section of Cellular Signaling Department of Molecular Biophysics and Physiology, Rush University School of Medicine, 1750 W. Harrison St, Chicago, IL 60612, - - 2013
The buffering power, B, of the sarcoplasmic reticulum (SR), ratio of the changes in total and free [Ca(2+)], was determined in fast-twitch mouse muscle cells subjected to depleting membrane depolarization. Changes in total SR [Ca(2+)] were measured integrating Ca(2+) release flux, determined with a cytosolic [Ca(2+)] monitor. Free [Ca(2+)](SR) was ...
Corre Jean-Philippe - - 2012
Background. Germination is a key step for successful B. anthracis colonisation and systemic dissemination. Few data are available on spore germination in vivo, and necessity of spore-host-cell interactions to initiate germination is unclear.Methods. To investigate the early interactions between B. anthracis spores and the cutaneous tissue, spores were inoculated in an intraperitoneal ...
Borin-Crivellenti S - - 2012
OBJECTIVE: To investigate the feasibility and validity of sampling blood from the carpal pad in hospitalised healthy and diabetic dogs. METHODS: The carpal pad was compared to the ear as a sampling site in 60 dogs (30 healthy and 30 diabetic dogs). RESULTS: Lancing the pads was very well tolerated. ...
Tamada Tsutomu - - 2012
PURPOSE: To establish a simple method to evaluate the degree of liver parenchymal enhancement in the hepatobiliary phase (HP) of gadoxetic acid-enhanced magnetic resonance imaging (MRI). MATERIALS AND METHODS: Subjects comprised 75 patients with or without chronic liver disease who underwent gadoxetic acid-enhanced MRI and indocyanine green retention at 15 ...
Estevez-Garcia Purificacion - - 2012
ABSTRACT: BACKGROUND: Angiogenesis plays an essential role in tumor growth and metastasis, and is a major target in cancer therapy. VEGFR and PDGFR are key players involved in this process. The purpose of this study was to assess the incidence of genetic variants in these receptors and its potential clinical ...
Gielen Andrea C AC Johns Hopkins Center for Injury Research and Policy, Baltimore, MD 21205, USA. - - 2012
Living in substandard housing may be one factor that increases the risk of fire and burn injuries in low-income urban environments. The purposes of this study are to (1) describe the frequency and characteristics of substandard housing in urban homes with young children and (2) explore the hypothesis that better ...
Fan Jianquan - - 2012
In this study, an novel amphiphilic block copolymer P[(DEMA-co-APMA)- b-PEGMA]-FA and its crosslinker Uracil-(CH2)6-Uracil (U-(CH2)6-U) were synthesized and used as the targeted and pH-responsive nano-carriers for anticancer drug delivery. The hydrophobic block of the copolymer contains adenine (A) and tertiary amine moieties and the hydrophilic block is terminated with a ...
Zhang Mei M Clinical Pharmacology, Department of Medicine, University of Otago-Christchurch, Christchurch, New Zealand. - - 2013
A rapid and simple high-performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of three triazole antifungals (voriconazole, posaconazole, and itraconazole and the metabolite of itraconazole, hydroxyitraconazole) in human plasma. Sample preparation involved a simple one-step protein precipitation with 1.0 M perchloric acid and methanol. After centrifugation, the ...
Gemma Sandra - - 2012
The intramolecular hydrogen bond formed between a protonated amine and a neighbouring H-bond acceptor group in the side chain of amodiaquine and isoquine is thought to play an important role in their antimalarial activities. Here we describe isoquine-based compounds in which the intramolecular H-bond is mimicked by a methylene linker. ...
Pubill-Ulldemolins Cristina - - 2012
The use of phosphines to assist the organocatalytic β-boration reaction of α,β-unsaturated carbonyl compounds has been demonstrated with a selected number of substrates. The new method eludes the use of Brönsted bases to promote the catalytic active species and PR(3) becomes essential to interact with the substrate resulting in the ...
Schmidt Stine Nørgaard - - 2012
Passive dosing applies a polymer loaded with test compound(s) to establish and maintain constant exposure in laboratory experiments. Passive dosing with the silicone poly(dimethylsiloxane) was used to control exposure of the terrestrial springtail Folsomia candida to six polycyclic aromatic hydrocarbons (PAHs) in bioconcentration and toxicity experiments. Folsomia candida could move ...
Austin Wayne R - - 2012
Nucleotide deficiency causes replication stress (RS) and DNA damage in dividing cells. How nucleotide metabolism is regulated in vivo to prevent these deleterious effects remains unknown. In this study, we investigate a functional link between nucleotide deficiency, RS, and the nucleoside salvage pathway (NSP) enzymes deoxycytidine kinase (dCK) and thymidine ...
Hynes Matthew J - - 2012
This work investigates self-assembled monolayers that were formed from a glycol-terminated thiol monomer and were patterned using photo-induced monolayer desorption. Utilizing direct-write photolithography provided a facile means to generate complex protein patterns containing gradients and punctate regions. The ablated glycol monolayers were characterized using scanning probe microscopy, which allowed us ...
Raigoza Annette F - - 2012
Peptide-terminated monolayers were formed through a Huisgen clycloaddition reaction between an α-helical peptide containing two propargylglycine unnatural functional groups 20 Å apart and an alkanethiol self-assembled monolayer (SAM) on a gold surface containing 25% surface density of reactive azide terminal groups. The azide- and peptide-terminated sur-faces were imaged by scanning ...
Cooney M T - - 2012
Background: Despite the fact that subjects with established coronary heart disease (CHD) are at high risk of further events and deserve meticulous secondary prevention, current audits such as EUROASPIRE show poor control of major risk factors. Ongoing monitoring is required. We present a new risk factor audit system, SURF (Survey ...
van Dorp Willem F - - 2012
Electron-induced chemistry on surfaces plays a key role in focused electron beam induced processing (FEBIP), a single-step lithography technique that has increasingly gained interest in the past decade. It is crucial for the understanding and modelling of this process to know the role of the surface in the electron-induced dissociation ...
Li Xijing - - 2012
This Note describes a practical and efficient procedure that takes advantage of diarylborinic acids as a cost-effective alternative to arylboronic acids for synthesis of diarylmethanes through metal-free reductive cross-coupling with N-tosylhydrazones of aromatic aldehydes and ketones. The procedure tolerates hydroxyl, halide, amine, and allyl functionality, complementary to the transition-metal catalyzed ...
Zhou Jun - - 2012
A label-free, non-enzyme immunosensing strategy is designed for ultrasensitive electronic detection of disease-related proteins (carcinoembryonic antigen as a model) by using gold nanoparticle-based bio-bar codes and an in situ amplified DNA-based hybridization chain reaction.
Kunst Stefanie - - 2012
Photoreceptor cells face the challenge of adjusting their function and, possibly, their susceptibility to light damage to the marked daily changes in ambient light intensity. In order to achieve a better understanding of photoreceptor adaptation at the transcriptional level, this study aimed to identify genes which are under daily regulation ...
Berthon Bronwyn S - - 2012
SUMMARY AT A GLANCE: This cross-sectional study investigated diet, inflammation, lung function and asthma symptoms in Australians asthmatics and healthy controls. Participants with severe persistent asthma showed a different dietary intake pattern compared with healthy controls, which was associated with lower lung function and increased airway inflammation. Asthmatics also had ...
Cormier Susan M - - 2012
Increased ionic concentrations are associated with the impairment of benthic invertebrate assemblages. However, the causal nature of that relationship must be demonstrated so that it can be used to derive a benchmark for conductivity. The available evidence is organized in terms of six characteristics of causation: co-occurrence, preceding causation, interaction, ...
Kinnevey Peter M - - 2013
Methicillin-resistant Staphylococcus aureus (MRSA) has been a major cause of nosocomial infection in Irish hospitals for 4 decades, and replacement of predominant MRSA clones has occurred several times. An MRSA isolate recovered in 2006 as part of a larger study of sporadic MRSA exhibited a rare spa (t878) and multilocus ...
Xu Xin - - 2012
The seventh type of Wells-Dawson isomer, δ-[(WO(5))W(17)Cu(H(2)O)O(55)](10-) () was obtained as a consequence of the formation of the first central square-pyramidal [WO(5)] moiety that results in a 45° rotation of the four belt [CuO(5)]/[WO(5)] square pyramids, establishing a new type of WD isomerism involving the rotation of the belt polyhedra, ...
Joshi Sucheta M - - 2012
A substantial minority of children with epilepsy have continued seizures despite adequate trials of standard antiseizure medications. To maximize seizure control and thereby optimize their neurodevelopmental outcomes, alternate nonmedication therapies should be considered for these patients. Dietary therapies, including the ketogenic diet and its variations, have been available for years. ...
Chow J P H - - 2012
Inhibition of cyclin-dependent kinase 1 (CDK1) by phosphorylation is a key regulatory mechanism for both the unperturbed cell cycle and the DNA damage checkpoint. Although both WEE1 and MYT1 can phosphorylate CDK1, little is known about the contribution of MYT1. We found that in contrast to WEE1, MYT1 was not ...
Hashimoto Shoko S Research Center for Environmental Bioscience and Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Hyogo, Sanda 669-1337, - - 2013
Protein-disulfide isomerase (PDI) is a dithiol/disulfide oxidoreductase that regulates the redox state of proteins. We previously found that overexpression of PDI in rat pituitary tumor (GH3) cells suppresses 3,3',5-triiodothyronine (T(3))-stimulated growth hormone (GH) expression, suggesting the contribution of PDI to the T(3)-mediated gene expression via thyroid hormone receptor (TR). In ...
Collu Francesca - - 2012
Basic understanding of the means by which multidrug efflux systems can efficiently recognize and transport drugs constitutes a fundamental step toward development of compounds able to tackle the continuous outbreak of new bacterial strains resistant to traditional antibiotics. We applied a series of computational techniques, from molecular docking to molecular ...
Agnarsson I - - 2013
The consequences of population subdivision and inbreeding have been studied in many organisms, particularly in plants. However, most studies focus on the short-term consequences, such as inbreeding depression. To investigate the consequences of both population fragmentation and inbreeding for genetic variability in the longer term, we here make use of ...
Tajima Naoshi - - 2012
A 65-year-old man was referred to our hospital due to epigastric pain. Abdominal enhanced computed tomography (CT) demonstrated marked dilatation of the main pancreatic duct (MPD) and communication to the gastric and duodenal lumen was suspected. Esophagogastroduodenoscopy (EGD) showed a villous tumor with white mucous discharge in the posterior wall ...
Nishi Mitsumi - - 2012
The reaction of α-thioketones and alkynes in the presence of a rhenium catalyst, [HRe(CO)(4)](n), gave γ-thio-α,β-unsaturated ketones in excellent yields. The alkynes were inserted into the carbon-sulfur bond of the α-thioketones, and isomerization of a double bond provided the products with high regio- and stereoselectivities. This reaction also proceeded in ...
Bailey-Davis Lisa - - 2012
OBJECTIVES:To examine 3-year trends and spatial clustering in the prevalence of obesity among elementary-aged children in Pennsylvania.METHODS:Height and weight were measured for ∼980 000 children between ages 5 and 12 years, corresponding to kindergarten through grade 6 in 3 consecutive school years (2006-2007, 2007-2008, 2008-2009). These data were obtained at the ...
Comer Jeffrey J Department of Physics and Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, - - 2012
Through all-atom molecular dynamics simulations, we explore the use of nanopores in thin synthetic membranes for detection and identification of DNA binding proteins. Reproducing the setup of a typical experiment, we simulate electric field driven transport of DNA-bound proteins through nanopores smaller in diameter than the proteins. As model systems, ...
Visavachaipan Nipapat N Division of Pediatric Endocrinology and Diabetes, New York University School of Medicine, New York, New York 10016, - - 2013
Acute lymphoblastic leukemia (ALL) maintenance therapy (MT) has been occasionally associated with symptomatic hypoglycemia (SH), attributed to purine analog (mercaptopurine [6-MP]). This hypoglycemia has been hypothesized to affect substrate utilization of gluconeogenic precursor alanine in the liver. An overweight 5-year-old boy with ALL was evaluated for SH (lethargy and vomiting) ...
Huxter M J - - 2012
ACCESSIBLE SUMMARY: Throughout recorded history, the mentally ill have been living at the fringes of society, frequently alienated, treated inhumanely, and made as scapegoats for the prevailing societal ills. Australia has seen the number of public psychiatric hospital beds fall from 30 000 in the early 1960's to 8000 as of ...
Cui Jiehu - - 2012
A highly connected 3D metal-organic framework with topology based on Zn(6)O(2) clusters and flexible carboxylate ligands has been synthesized. The obtained Zn-MOF shows solvatochromic behavior for fluorescence sensing of small molecules, gas adsorption properties and exceptional chemical stability and might have applications for separation and detection purposes.
Lee Haeyeong H Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, - - 2013
We sought to characterize molecular expression and ionic conductances in a novel population of interstitial cells (PDGFRα(+) cells) in murine bladder to determine how these cells might participate in regulation of detrusor excitability. PDGFRα(+) cells and smooth muscle cells (SMCs) were isolated from detrusor muscles of PDGFRα(+)/eGFP and smMHC/Cre/eGFP mice ...
Coronado Eugenio - - 2012
In this review we combine the use of coordination chemistry with the concepts of molecular magnetism to design magnetic Metal-Organic Frameworks (MOFs) in which the crystalline network undergoes a dynamic change upon application of an external stimulus. The various approaches so far developed to prepare these kinds of chemically or ...
Siciliano Carlo - - 2012
The study describes a new "one-pot" route to the synthesis of N-9-fluorenylmethyloxycarbonyl (Fmoc) α-amino diazoketones. The procedure was tested on a series of commercially available free or side-chain protected α-amino acids employed as precursors. The conversion into the title compounds was achieved by masking and activating the α-amino acids with ...
Fox C W - - 2012
Little is known about how inbreeding alters selection on ecologically relevant traits. Inbreeding could affect selection by changing the distribution of traits and/or fitness, or by changing the causal effect of traits on fitness. Here, I test whether selection on egg size varies with the degree of inbreeding in the ...
Nagahara Noriyuki - - 2012
Significance: Redox regulates 3-mercaptopyruvate sulfurtransferase (MST, EC 2.8.1.2) activity via both intermolecular and intramolecular redox-sensing switches. The intermolecular switch comprises an intermolecular disulfide bond that forms a homodimer. On the other hand, the intramolecular switch is a catalytic site cysteine that forms a low redox potential sulfenate. Both switches are ...
Pratlong Francine - - 2012
SUMMARY A series of 2277 Leishmania strains from Old World visceral leishmaniasis foci, isolated between 1973 and 2008, were studied by isoenzyme analysis. The strains were obtained from humans, domestic and wild carnivores, rodents and phlebotomine sandflies, and came from 36 countries. In all, 60 different zymodemes were identified and ...
Stam Anine H AH Department of Neurology, Leiden University Medical Centre, The - - 2013
Migraine, in particular with aura, has been associated with an increased risk for ischemic stroke and coronary heart disease. The underlying mechanism is unknown. In a cross-sectional case control study we investigated whether an enhanced risk of atherosclerosis in migraineurs explains this increased cardiovascular risk. Subjects were participants from the ...
Lambrinoudaki I - - 2012
Objective To assess the interaction of the MTHFR C677T polymorphism with changes in lipid and glucose metabolism effected by oral hormone replacement therapy (HRT) in postmenopausal women. Methods In this open-label, prospective, interventional study, parameters of lipid and glucose metabolism, as well as homocysteine, were assessed in 97 postmenopausal women ...
Giles Logan J - - 2012
In this work, we demonstrate the potential of multi-edge X-ray absorption near-edge structure (XANES) analysis in completely defining the ground state electronic structure of a prototypical biomimetic complex of the [2Fe]-subcluster of the catalytic H-cluster of FeFe-hydrogenase. The spectral features at the ionization thresholds for Fe, S, C and O ...
Paul Kush K Department of Molecular and Integrative Physiology, University of Illinois, Urbana, IL 61801, USA. - - 2013
Fragile X syndrome (FXS) is the most common form of inheritable mental retardation caused by transcriptional silencing of the Fmr1 gene resulting in the absence of fragile X mental retardation protein (FMRP). The role of this protein in neurons is complex and its absence gives rise to diverse alterations in ...
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