Search Results
Results 601 - 650 of 2099
< 8 9 10 11 12 13 14 15 16 17 18 >
Spoerke Jill M - - 2012
Class 1 phosphatidylinositol 3-kinase (PI3K) plays a major role in cell proliferation and survival in a wide variety of human cancers. Here, we investigated biomarker strategies for PI3K pathway inhibitors in non-small-cell lung cancer (NSCLC). Molecular profiling for candidate PI3K predictive biomarkers was conducted on a collection of NSCLC tumor ...
Ashikov Angel - - 2012
LARGE (like-glycosyltransferase) and LARGE2 (glycosyltransferase-like 1B, Gyltl1b) are homologous Golgi glycosyltransferases possessing two catalytic domains with homology to members of glycosyltransferase families GT8 and GT49. Mutations in human and mouse Large result in muscular dystrophy due to underglycosylation of dystroglycan. The systemic function of LARGE2 is unknown, but at cellular ...
Zeng Chunhua - - 2012
The cochlear nucleus (CN) receives innervation from auditory and somatosensory structures, which can be identified using vesicular glutamate transporters, VGLUT1 and VGLUT2. VGLUT1 is highly expressed in the magnocellular ventral CN (VCN), which receives auditory nerve inputs. VGLUT2 is predominantly expressed in the granule cell domain (GCD), which receives nonauditory ...
Gosselin-Badaroudine Pascal - - 2012
Mammalian voltage-gated sodium channels are composed of four homologous voltage sensor domains (VSDs; DI, DII, DIII, and DIV) in which their S4 segments contain a variable number of positively charged residues. We used single histidine (H) substitutions of these charged residues in the Na(v)1.4 channel to probe the positions of ...
Chen Yu-Chi - - 2012
Trans-synaptic adhesion between Neurexins (Nrxs) and Neuroligins (Nlgs) is thought to be required for proper synapse organization and modulation, and mutations in several human Nlgs have shown association with autism spectrum disorders. Here we report the generation and phenotypic characterization of Drosophila neuroligin 2 (dnlg2) mutants. Loss of dnlg2 results ...
Nakamura Mitsunobu - - 2012
The binding and fluorescence properties of complementary RNA sequences attached to different numbers of pyrenes via one carbon linker at the 2'-O-positions have been investigated. Upon hybridization of the pyrene-modified RNA sequences, the modified RNA duplexes with normal thermal stability are formed, and the pyrene arrays are assembled in an ...
Bundhun Ashwini - - 2012
A systematic investigation is carried out using the B3LYP, BLYP, and BHLYP functionals and MP2 level of theory to characterize the low-lying electronic singlet and triplet GeC(2)N(2) isomers. The basis sets used are of double-ζ plus polarization quality with additional s- and p-type diffuse functions, DZP++. Three bent isomers Ge(CN)(2), ...
Unger Bethany - - 2013
Vaccinia virus membrane biogenesis requires the A14 and A17 proteins. We show here that both proteins can associate with membranes co- but not posttranslationally, and we perform a structure function analysis of A14 and A17 using inducible recombinants. In the absence of A14, electron-dense virosomes and distinct clusters of small ...
Kusama Nobuyoshi N Dept. of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, - - 2013
Acid-sensing ion channels (ASICs) are sodium channels gated by extracellular protons. ASIC1a channels possess intersubunit Cl(-)-binding sites in the extracellular domain, which are highly conserved between ASIC subunits. We previously found that anions modulate ASIC1a gating via these sites. Here we investigated the effect of anion substitution on native ASICs ...
Ben Hamida Sami - - 2012
Uncontrolled consumption of alcohol is a hallmark of alcohol abuse disorders; however, the central molecular mechanisms underlying excessive alcohol consumption are still unclear. Here, we report that the GTP binding protein, H-Ras in the nucleus accumbens (NAc) plays a key role in neuroadaptations that underlie excessive alcohol-drinking behaviors. Specifically, acute ...
Clarridge Jill E JE - - 2013
The anterior nares are the site of choice for the Veterans Administration methicillin-resistant Staphylococcus aureus (MRSA) surveillance program; however, a correlation between nares colonization and concomitant wound infections has not been well established. The purpose of this study was 3-fold: to determine the relatedness of MRSA isolates from 40 paired ...
Pek Jun Wei - - 2012
In Drosophila, Maelstrom is a conserved component of the perinuclear nuage, a germline-unique structure that appears to serve as a site for Piwi-interacting RNA (piRNA) production to repress deleterious transposons. Maelstrom also functions in the nucleus as a transcriptional regulator to repress the expression of microRNA-7, a process that is ...
Gergidis Leonidas N - - 2012
The structural properties of polymer brushes, formed by dendron polymers up to the third generation, were studied by means of Brownian dynamics simulations for the macroscopic state of good solvent. The distributions of polymer units, of the free-ends, of the dendrons centers of mass and of the units of every ...
Herzog Gal G Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, - - 2012
The transcription factor p53 is a key tumor suppressor protein. In about half of human cancers, p53 is inactivated directly through mutation in its sequence-specific DNA-binding domain. Drosophila p53 (Dmp53) has similar apoptotic functions as its human homolog and is therefore an attractive model system for studying cancer pathways. To ...
Freund Dana M DM Dept. of Biochemistry and Molecular Biology, Colorado State Univ., Ft. Collins, CO 80523-1870, - - 2013
Metabolic acidosis is a common clinical condition that is caused by a decrease in blood pH and bicarbonate concentration. Increased extraction and mitochondrial catabolism of plasma glutamine within the renal proximal convoluted tubule generates ammonium and bicarbonate ions that facilitate the excretion of acid and partially restore acid-base balance. Previous ...
Li Jie-Yuan - - 2012
Recent studies in normal subjects suggested the callosal motor fibers pass through the posterior body of the corpus callosum (CC) but this has not been tested in patients with callosal infarction. The objective of the study is to define the pathways involved in transcallosal inhibition by examining patients with infarctions ...
Wille Coral K - - 2013
The Epstein-Barr virus (EBV) immediate-early proteins BZLF1 and BRLF1 can both induce lytic EBV reactivation when overexpressed in latently infected cells. Although EBV genome methylation is required for BZLF1-mediated activation of lytic gene expression, the effect of viral genome methylation on BRLF1-mediated viral reactivation has not been well studied. Here, ...
Jin Hekun - - 2012
The degradation of basement membranes by tumor cells involves secretion and activation of proteinases, such as the matrix metalloproteinases (MMPs), and results from an imbalance between their inhibitors and activators that are controlled by various growth factors or cytokines, among which TGF-β1 may be the most intriguing. In order to ...
Ma Shao-Gang - - 2012
The potential roles of serum butyrylcholinesterase (BChE) activity and platelet indices in type 1 diabetes (T1D) remain uncertain. We aimed to investigate the correlation among the platelet indices, serum BChE activity, and diabetic ketoacidosis (DKA). Sixty-one T1D patients, 29 patients with DKA, and 30 age- and sex-matched controls were enrolled. ...
Dambach Donna M - - 2012
Widespread sharing and analysis of clinical trial data and a U.S. government initiative to engineer nonclinical cell-based models that mimic human biological processes have the potential to improve predictions of drug-related adverse events.
LaSala P Rocco - - 2013
Quantitative fecal lactoferrin was measured in 112 patients tested for toxigenic Clostridium difficile using glutamate dehydrogenase (GDH) and toxin immunoassays combined with tcdB PCR. Lactoferrin levels were higher in the GDH-positive/toxin-positive group (79 μg/ml) than in the GDH-positive/toxin-negative/PCR-positive (21 μg/ml) and the GDH-negative groups (13 μg/ml). Differences in fecal lactoferrin ...
Spalding Roy F - - 2012
TransCanada's proposed international crude oil pipeline route over sensitive, relatively pristine, sub-irrigated land underlain by the Ogallala aquifer led to increased scrutiny and eventual rejection of the Keystone XL pipeline. Pipeline routing could be made much more acceptable by adopting risk-managed routes that lessen the potential to adversely impact high-quality ...
Ghosh Santanu - - 2012
An intramolecular-dehydrogenative-coupling (IDC) using "transition-metal-free" oxidation conditions has been achieved to synthesize a variety of 2-oxindoles bearing an all-carbon quaternary stereogenic center at the benzylic position. The methodology involves a one-pot C-alkylation of β-N-arylamido esters (3, 6) with alkyl halides using potassium tert-butoxide concomitant with a dehydrogenative coupling. A radical-mediated ...
Xu Xing-Dong - - 2012
A series of new platinum-acetylide complexes containing ethynyl-pyrene moieties as the main skeleton were synthesized and characterized. The investigation of the absorption and emission spectra of these complexes revealed that the extension of the molecular size with the introduction of different numbered platinum-acetylide fragments can efficiently tune the absorption and ...
Simeček Jakub - - 2012
To assess the influence of Zn(2+) , Cu(2+) , Fe(3+) , Al(3+) , Ti(IV) , and Sn(IV) on incorporation of (68) Ga(3+) into pendant-arm macrocyclic chelators, the (68) Ga labeling of 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), 1,4,7-triazacyclononane-1,4,7-tris[methyl(2-carboxyethyl)phosphinic acid]) (TRAP), and 1,4,7-triazacyclononane-1-[methyl(2-carboxyethyl)phosphinic acid]-4,7-bis[methyl(2-hydroxymethyl)phosphinic acid] (NOPO), as well as their peptide ...
Mähler Johan - - 2012
The bond distances in hydrated arsenic oxyacid species in aqueous solution have been studied by EXAFS spectroscopy and large angle X-ray scattering, LAXS. These results have been compared to structures in the solid state, as found in an extensive survey of available crystal structures. Protonated oxygen atoms can be distinguished ...
Bell Stephen G - - 2012
The cytochrome P450 enzyme CYP199A4, from Rhodopseudomonas palustris HaA2, can efficiently demethylate 4-methoxybenzoic acid. It is also capable of oxidising a range of other related substrates. By investigating substrates with different substituents and ring systems we have been able to show that the carboxylate group and the nature of the ...
Joshi Ravi- - - 2012
Structures, hydrogen bonding and binding energies of H4SeA (A = O, S, Se) dimers and their cation radicals have been studied using DFT-B3LYP, MP2 and CCSD methods with 6-31++G(d,p), cc-pVTZ and aug-cc-pVTZ basis sets. The binding energy (BE) order of the most stable neutral and cationic dimers have been found ...
Filippi Luca - - 2012
Abstract Background: Fluorodeoxygluose (FDG) positron emission/computed tomography (PET/CT) is emerging as a useful tool for the diagnosis of peritoneal carcinomatosis (PC). In this study, we assessed whether dual-time point imaging can improve the accuracy of FDG PET/CT for the diagnosis of PC after colon rectal cancer (CRC). Methods: Thirty-nine patients ...
Pardey Margery C - - 2012
Impulsivity is characteristic of several mental health disorders and is largely mediated by the prefrontal cortex subregions: the medial prefrontal cortex (mPFC) and the orbitofrontal cortex (OFC). Dopamine (DA) and norepinephrine (NE) are known to modulate activity of the prefrontal cortex, however their direct role in impulsive choice is not ...
Miller R Lance RL Eptihelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, - - 2013
The peptide hormone arginine vasopressin (AVP) plays a critical role in regulating salt and water transport in the mammalian kidney. Recent studies have also demonstrated that AVP can promote cell survival in neuronal cells through V1 receptors. The current study addresses whether AVP can inhibit apoptosis in kidney collecting duct ...
Ji Weiqing W Dept. of Biology, Washington Univ, St. Louis, MO 63130, USA. - - 2013
In the auditory system of the big brown bat (Eptesicus fuscus), paired conditioned tonal (CS) and unconditioned leg stimuli (US) for auditory fear conditioning elicit tone-specific plasticity represented by best-frequency (BF) shifts that are augmented by acetylcholine, whereas unpaired CS and US for pseudoconditioning elicit a small BF shift and ...
Jaroentomeechai Thapakorn - - 2012
Novel phthalimide and o-sulfobenzimide-functionalized silsesquioxanes were successfully synthesized via nucleophilic substitution reactions from octakis(3-chloropropyl)octasilsesquioxane. Surprisingly, the formation of deca- and dodecasilsesquioxanes cages was discovered during substitution with phthalimide, but only octasilsesquioxane maintained a cage in the o-sulfobenzimide substitution reaction. Moreover, we report the electronic effect of nitrogen nucleophiles to promote ...
Spetea Mariana - - 2012
Here we report on the design, synthesis, and biological characterization of novel κ opioid (KOP) receptor ligands of diphenethylamines. In opioid receptor binding and functional assays, the N-cyclobutylmethyl substituted derivative 4 (HS665) showed the highest affinity and selectivity for the KOP receptor and KOP agonist potency. Compound 4 inhibited acetic ...
Mori Kumiko - - 2012
BACKGROUND:Telomerase is regarded as a good marker for cancer because it is unregulated in most tumor cells compared with normal cells. We evaluated telomerase activity in the lysate of tumor tissue and surrounding cells of oral cancer patients by an electrochemical technique, dubbed the electrochemical telomerase assay (ECTA).METHODS:The assay used ...
Drake Isabel - - 2012
BACKGROUND: Dietary carbohydrates have been implicated in relation to prostate cancer. OBJECTIVE: Our objective was to examine the associations between dietary intakes of carbohydrates, fiber, and their food sources and risk of prostate cancer, overall and by case severity, in the Malmö Diet and Cancer cohort. DESIGN: The analysis included ...
Kobayashi Atsushi - - 2012
BACKGROUND:The early stages of spondylolysis are extremely difficult to diagnose on plain radiography. Although several studies have examined changes in active spondylolysis on magnetic resonance imaging (MRI), no studies to date have determined the onset frequency of active spondylolysis detectable on MRI but occult on plain radiography. Moreover, the clinical ...
Nomura Motonari - - 2012
Hemagglutinating virus of Japan-envelope (HVJ-E) is a drug delivery vector based on inactivated Sendai virus. Recently, anti-tumor activities were found for HVJ-E itself, and clinical trials of HVJ-E for some malignant tumors are now ongoing. We investigated the in vitro and in vivo antitumor effects of HVJ-E against neuroblastoma, which ...
Nakamura Kae K Department of Physiology, Kansai Medical University, Fumizono-cho, Moriguch City, Osaka 570-8506, Japan. - - 2012
The basal ganglia play a pivotal role in reward-oriented behavior. The striatum, an input channel of the basal ganglia, is composed of subdivisions that are topographically connected with different cortical and subcortical areas. To test whether reward information is differentially processed in the different parts of the striatum, we compared ...
Callahan Margaret K MK Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, - - 2012
Vemurafenib, a selective RAF inhibitor, extends survival among patients with BRAF V600E-mutant melanoma. Vemurafenib inhibits ERK signaling in BRAF V600E-mutant cells but activates ERK signaling in BRAF wild-type cells. This paradoxical activation of ERK signaling is the mechanistic basis for the development of RAS-mutant squamous-cell skin cancers in patients treated ...
Ray Bimal K - - 2012
A disintegrin and metalloprotease domain-containing protein 12 (ADAM12) is up-regulated in many human cancers and promotes cancer metastasis. Increased urinary level of ADAM-12 in breast and bladder cancers correlates with the disease progression. However, the mechanism of its induction in cancer remains less understood. Previously, we reported a Z-DNA forming ...
Shen Weiyong W Save Sight Institute, The University of Sydney, Sydney 2000, Australia. - - 2012
Müller cells are the major glia of the retina that serve numerous functions essential to retinal homeostasis, yet the contribution of Müller glial dysfunction to retinal diseases remains largely unknown. We have developed a transgenic model using a portion of the regulatory region of the retinaldehyde binding protein 1 gene ...
Bings Nicolas Hubert - - 2012
Atomic spectrometry remains one of the most powerful methods for the determination of the elements and their compounds in a wide variety of types of samples and in widely differing concentrations. Innovation in the optical methods being atomic absorption, atomic fluorescence, and atomic emission spectrometry mainly with plasma sources as ...
Yin Zhou - - 2012
Phosphorylation-dependent protein-protein interaction has rarely been targeted in medicinal chemistry. Thymoquinone, a naturally occurring antitumor agent, disrupts pre-phosphorylated substrate recognition by the polo-box domain of polo-like kinase 1, a key mitotic regulator responsible for various carcinogenesis when over-expressed. Here, crystallographic studies reveal that the phosphoserine/phosphothreonine recognition site of the polo-box ...
Meyerhardt Jeffrey A JA Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA 02215, USA. - - 2012
The influence of glycemic load and related measures on survival among colon cancer patients remains largely unknown. We conducted a prospective, observational study of 1011 stage III colon cancer patients reporting dietary intake during and 6 months after participation in an adjuvant chemotherapy trial. We examined the influence of glycemic ...
Lim Ratana - - 2012
Preterm birth is a major determinant of neonatal mortality and morbidity with approximately one third due to pre-labor rupture of membranes. Infection and inflammation have strong causal links to preterm delivery, resulting in the activation of nuclear factor-kappaB (NFKB) and its downstream targets. Human sirtuin (SIRT) 6, which has ADP-ribosyl ...
King Deborah F L DF Centre for Infection, Clinical Sciences, St. George's University of London, London, United - - 2013
Human immunodeficiency virus type 1 (HIV-1) transmission results from infection with one or a small number of variants from the donor quasispecies. Transmitted/founder (T/F) viruses have recently been identified from acutely infected patients, but the way in which they interact with primary targets of HIV-1 infection is poorly understood. We ...
Cestari Igor I Seattle Biomedical Research Institute, Seattle, WA, - - 2013
Aminoacyl-tRNA synthetases are enzymes that charge specific tRNAs with their cognate amino acids and play an essential role in the initial steps of protein synthesis. Because these enzymes are attractive targets for drug development in many microorganisms, there is a pressing need for assays suitable for compound screening. We developed ...
Chen Xuanmao X Department of Pharmacology, School of Medicine, School of Public Health, University of Washington, Seattle, Washington 98195-7750, - - 2012
Cilia of olfactory sensory neurons are the primary sensory organelles for olfaction. The detection of odorants by the main olfactory epithelium (MOE) depends on coupling of odorant receptors to the type 3 adenylyl cyclase (AC3) in olfactory cilia. We monitored the effect of airflow on electro-olfactogram (EOG) responses and found ...
Hira Vishal - - 2013
Coagulase-negative staphylococci (CoNS) isolated in neonatal late-onset sepsis are often antibiotic resistant. We analyzed CoNS from skin and feces of neonates during hospitalization. Antibiotic resistance of skin isolates increased during hospitalization, especially in Staphylococcus haemolyticus. Staphylococcus warneri showed low antibiotic resistance. Our data suggest that different CoNS species may play ...
< 8 9 10 11 12 13 14 15 16 17 18 >