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Results 301 - 350 of 1352
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Brill Shlomo - - 2012
EmrE, a multidrug antiporter from Escherichia coli, has presented biochemists with unusual surprises. Here we describe the transformation of EmrE, a drug/H(+) antiporter to a polyamine importer by a single mutation. Antibiotic resistance in microorganisms may arise by mutations at certain chromosomal loci. To investigate this phenomenon, we used directed ...
Gabreyes Alemayehu A - - 2012
Copper is an essential trace element that is involved in a number of important enzymatic processes throughout the body. Recent single case reports and small studies have shown that deficiency of copper can cause reversible haematological changes and irreversible neurological injury. We chose to undertake a national study, looking at ...
Wei Li - - 2012
Virus infection activates host cellular signaling pathways, including the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, which regulates diverse cellular activities related to cell growth, survival, and apoptosis. The present study demonstrated for the first time that porcine circovirus type 2 (PCV2), a major causative agent of postweaning multisystemic wasting syndrome, which is ...
Chauhan Preeti - - 2012
Using a multimeasure longitudinal research design, we measured psychopathy with the Youth Psychopathic Traits Inventory (YPI) and the Psychopathy Checklist-Youth Version (PCL-YV) among 122 offending girls. We examined the psychometric properties of the YPI, investigated the association between the YPI and the PCL-YV, and assessed their concurrent and longitudinal association ...
Asaad Wael F WF Department of Neurobiology, The University of Chicago, Chicago, Illinois 60637, - - 2013
Behavioral, psychological, and physiological experiments often require the ability to present sensory stimuli, monitor and record subjects' responses, interface with a wide range of devices, and precisely control the timing of events within a behavioral task. Here, we describe our recent progress developing an accessible and full-featured software system for ...
Modur Vijay - - 2012
BACKGROUND:The promise of targeted therapies in molecularly defined subsets of cancer has led to a transformation of the process of drug development in oncology. To target cancer successfully and precisely requires high-quality translational data. Such data can be generated by the use of biomarkers that answer key questions in drug ...
Kim Andrea A - - 2012
Background: Honduras has one of the highest HIV prevalence rates in Central America. Data on HIV incidence is needed to identify groups at greatest need of prevention interventions to inform the HIV response. Methods: We applied a test for recent infection to HIV-positive specimens from a biologic and behavioral survey ...
Chiu Tsan-Yu - - 2012
NTPDases (Apyrases) (EC 3.6.1.5) hydrolyze di- and triphosphate nucleotides, but not monophosphate nucleotides. They are categorized as E-type ATPases, have a broad divalent cation (Mg(2+), Ca(2+)) requirement for activation, and are insensitive to inhibitors of F-type, P-type, and V-type ATPases. Among the seven NTPDases identified in Arabidopsis, only APYRASE 1 ...
Mefford Heather C - - 2012
Advances in technology are rapidly changing the field of medical genetics in both the research laboratory and the clinic. With the use of next-generation, or massively parallel, DNA sequencing, it is possible to determine the sequence of essentially all genes in an individual's genome - referred to as the exome ...
Gwon Dong Il - - 2012
BackgroundAs bacterial attachment and subsequent biofilm formation on the surface of a stent lumen may be important in initiating sludge formation and subsequent stent blockage, there is a need for antibiotic-eluting stents to prevent sludge formation by bacterial attachment and colonization.PurposeTo evaluate the antimicrobial effects of cefotaxime-eluting covered self-expandable stents ...
Tamaki Hiroya - - 2012
Mismatched human leukocyte antigens (HLAs) on leukemic cells can be targeted by donor T cells in HLA-mismatched/haploidentical stem cell transplantation. In two cases of acute myeloid leukemia with t(6;11)(q27;q23) abnormality presented here, flow cytometry analysis showed a lack of HLA-A unshared between recipients and donors in relapsing leukemic cells after ...
Moniz Sónia - - 2012
The gene encoding protein kinase WNK2 was recently identified to be silenced by promoter hypermethylation in gliomas and meningiomas, suggesting a tumour suppressor role in these brain tumours. Following experimental depletion in cell lines, WNK2 was further found to control GTP-loading of Rac1, a signalling GTPase involved in cell migration ...
Lin Shen - - 2012
Microtubules are nearly uniformly oriented in the axons of vertebrate neurons but are non-uniformly oriented in their dendrites. Studies to date suggest a scenario for establishing these microtubule patterns whereby microtubules are transported into the axon and nascent dendrites with plus-ends-leading, and then additional microtubules of the opposite orientation are ...
Bautista-Garcia Pablo - - 2012
Zona occludens-2 (ZO-2) is a protein present at the tight junction and nucleus of epithelial cells. ZO-2 represses the transcription of genes regulated by the Wnt/β-catenin pathway. This pathway plays a critical role in podocyte injury and proteinuria. Here we analyze if the over-expression of ZO-2 in the glomerulus, by ...
Gal Joseph - - 2012
In 1886, Italian chemist Arnaldo Piutti isolated, for the first time, d-asparagine, the enantiomer of the known l-asparagine. He obtained 100 g of d-asparagine from 6500 kg of vetches. Using an ingenious synthetic scheme, Piutti established the chemical structure of asparagine and demonstrated that his isolation of d-asparagine from plants was not ...
Di Martino Maria T - - 2012
PURPOSE: Deregulated expression of microRNAs (miRNAs) has been demonstrated in multiple myeloma (MM). A promising strategy to achieve a therapeutic effect by targeting the miRNA regulatory network is to enforce the expression of miRNAs that act as tumor suppressor genes, such as miR-34a EXPERIMENTAL DESIGN: Here, we investigated the therapeutic ...
Ferreira Rui M - - 2012
The present report describes a novel method for genotyping the virulence-associated vacA intermediate (i) region of Helicobacter pylori in archive material. vacA i-region genotypes as determined by the novel method were completely concordant with those of sequence analysis and with those of functional vacuolation activity. The method was further validated ...
Mattei P L PL Department of Dermatology, Massachusetts General Hospital, Boston, MA, - - 2013
The cutaneous effects of rapidly accelerated fibrosarcoma kinase B (BRAF) inhibitors are not well understood. Squamous cell carcinoma (SCC), keratoacanthoma, and photosensitivity have been described in patients taking BRAF inhibitors. To characterize the timing and frequency of skin lesions in patients receiving BRAF inhibitor therapy, we utilized a retrospective case ...
Yu Shaoning S Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1055, - - 2012
Transduction of biological signals at the molecular level involves the activation and/or inhibition of allosteric proteins. In the transcription factor cAMP receptor protein (CRP) from Escherichia coli, the allosteric activation, or apo-holo transition, involves rigid body motions of domains and structural rearrangements within the hinge region connecting the cAMP- and ...
Burbrink Frank T FT Department of Biology, The College of Staten Island, The City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314, USA. - - 2012
Adaptive radiation (AR) theory predicts that groups sharing the same source of ecological opportunity (EO) will experience deterministic species diversification and morphological evolution. Thus, deterministic ecological and morphological evolution should be correlated with deterministic patterns in the tempo and mode of speciation for groups in similar habitats and time periods. ...
Matsubara Tsutomu T Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, - - 2012
Transforming growth factor-β (TGFβ) is activated as a result of liver injury, such as cholestasis. However, its influence on endogenous metabolism is not known. This study demonstrated that TGFβ regulates hepatic phospholipid and bile acid homeostasis through MAD homolog 3 (SMAD3) activation as revealed by lithocholic acid-induced experimental intrahepatic cholestasis. ...
Hu Chiung-Wen - - 2012
Areca nut is a carcinogen to human and has been strongly associated with oral pre-malignant and malignant diseases. Previous studies speculated the presence of unknown direct-acting mutagens present in aqueous extracts of areca nut. We hypothesized whether any direct-acting alkylating agents present in areca nut and its commercial products. In ...
Kole Goutam Kumar - - 2012
This tutorial review discusses recent literature on coordination polymers and metal-organic frameworks (MOFs) that exhibit solid-state reactivity and structural transformation under various experimental conditions. Removal or exchange of solvents and guest molecules, exposure to reactive vapours, and external stimuli such as heat, light or mechanochemical force cause such structural transformations ...
Fan Kai Y - - 2012
The symptoms of Parkinson's disease (PD) are related to changes in the frequency and pattern of activity in the reciprocally connected GABAergic external globus pallidus (GPe) and glutamatergic subthalamic nucleus (STN). In idiopathic and experimental PD, the GPe and STN exhibit hypoactivity and hyperactivity, respectively, and abnormal synchronous rhythmic burst ...
Davis Katherine E - - 2012
Episodic memory loss is a defining feature of early-stage Alzheimer's disease (AD). A test of episodic-like memory for the rat, the What-Where-Which occasion task (WWWhich), requires the association of object, location, and contextual information to form an integrated memory for an event. The WWWhich task cannot be solved by use ...
McLaughlin Nancy N Brain Tumor Center and Pituitary Disorders Program, John Wayne Cancer Institute at Saint John's Health Center, Santa Monica, California 90404, United - - 2012
Intraoperative navigation is an important tool used during endonasal surgery, which typically requires rigid head fixation. Herein we describe a navigational technique using an autoregistration mask without head fixation. Prospective evaluation of a surface autoregistration mask used without rigid head fixation in 12 consecutive endonasal endoscopic skull base procedures was ...
Cesaro Simone - - 2012
BACKGROUND: Peripheral blood stem cells (PBSCs) are the preferred source in autologous transplantation. We assessed prospectively the efficacy of mobilization in pediatric patients and risk factors associated with its failure. STUDY DESIGN AND METHODS: Patients, aged 0 to 17 years, needing a first collection of PBSCs for autologous stem cell ...
Hogg Kerstin - - 2012
Venous thromboembolism (VTE) is a serious and potentially fatal medical condition. Correct diagnosis and early treatment of VTE with anticoagulant drugs are critical steps in preventing further complications and recurrence. Evidence suggests that patients with suspected deep vein thrombosis (DVT) or pulmonary embolism (PE) should be managed with a diagnostic ...
Sevin Adrienne - - 2012
Sorafenib and sunitinib are oral tyrosine kinase inhibitors, commonly used in the treatment of metastatic renal cell carcinoma. Known adverse events associated with tyrosine kinase inhibitors include hypertension and palmarplantar erythrodysesthesia. We report two cases of acute pancreatitis associated with tyrosine kinase inhibitors.
Chew Ching Hoong - - 2012
Malaria remains one of the major killers of humankind and persists to threaten the lives of more than one-third of the world's population. Given that human malaria can now be caused by five species of Plasmodium, i.e., Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale, and the recently included Plasmodium ...
San Martin Loreto L Department of Physiology, Faculty of Biological Sciences, University of Concepcion, 403901 Concepcion, - - 2012
Gβγ interaction with GlyR is an important determinant in ethanol potentiation of this channel. A small peptide, RQH(C7), can inhibit ethanol potentiation of GlyR currents. Results with RQH(C7) indicate that ethanol mediated potentiation of GlyR is in part by Gβγ activation. Molecular interaction between Gβγ and GlyR could be used ...
Zacharia Brad E - - 2012
Symptomatic atherosclerotic intracranial arterial stenosis is a common etiologic mechanism of ischemic stroke. Patients with intracranial arterial stenosis carry a high risk of recurrent stroke that persists even with aggressive preventive measures.(1) The growing popularity of endovascular interventions for intracranial arterial stenosis, such as percutaneous transluminal angioplasty and stenting (PTAS), ...
Osman Nadir I - - 2012
Nocturia is a prevalent highly bothersome urinary symptom that may significantly detriment the health and wellbeing of sufferers. It is characterized by waking at night to void, each void preceded and followed by sleep, hence leads to fragmentation of sleep and day time tiredness. This may result in reduced productivity ...
Varraso Raphaëlle - - 2012
BACKGROUND: In the field of nutritional epidemiology, principal component analysis (PCA) has been used to derive patterns, but the robustness of interpretation might be an issue when the sample size is small. The authors proposed the alternative use of confirmatory factor analysis (CFA) to define such patterns. OBJECTIVE: The aim ...
Hu Xiaobang - - 2012
Multiple mechanisms contribute to a photoreceptor's ability to adapt to ambient light conditions. The mosquito Aedes aegypti expresses the long-wavelength rhodopsin Aaop1 in all R1-R6 photoreceptors and most R8 photoreceptors. These photoreceptors alter the cellular location of Aaop1 and reorganize their photosensitive rhabdomeric membranes on a daily basis. During daylight ...
Schweigert Michael - - 2012
Objective Necrotizing pneumonia, pulmonary abscess, and lung gangrene are rare complications of severe pulmonary infection with devitalization and sloughing of lung tissue. Pulmonary necrosis is often associated with alcoholism and other chronic disorders with known immunodeficiency. Mortality is significant and both treatment strategies as well as the role of surgery are ...
Gohil S - - 2012
We report a case of neuropathic pain following peripheral venous cannulation for an elective surgical procedure and discuss the various mechanisms by which this could occur. The mostly likely trigger in this case is phlebitis as the onset of symptoms coincided with the local infection. Neuropathic pain can occur following ...
Buoli Massimiliano - - 2012
BACKGROUND: Several studies show an association between a long duration of untreated illness (DUI) and poor outcome in schizophrenic patients. DUI, in turn, may be influenced by different variables including specific illness-related factors as well as access to local psychiatric services. AIMS: The purposes of the present study were to ...
Davis Mellar P - - 2012
Introduction: To develop a shorter and less burdensome cancer-related fatigue (CRF) questionnaire, the Four-Item Fatigue Scale (FIFS) was tested for validity against the Brief Fatigue Index (BFI). METHODS: On day 1, patients with cancer completed the BFI and FIFS in a random order and the reverse on day 7. Sixty-five ...
Skinner Michael K - - 2012
ABSTRACT: BACKGROUND: Environmentally induced epigenetic transgenerational inheritance of adult onset disease involves a variety of phenotypic changes, suggesting a general alteration in genome activity. RESULTS: Investigation of different tissue transcriptomes in male and female F3 generation vinclozolin versus control lineage rats demonstrated all tissues examined had transgenerational transcriptomes. The microarrays ...
Ruggiero Antonio - - 2012
BACKGROUND: The current study investigated the efficacy, safety, tolerability, and compliance of a transdermal buprenorphine delivery system for the management of chronic cancer pain in the pediatric population. PROCEDURE: Sixteen pediatric patients with moderate to severe cancer-related pain not satisfactorily controlled with previous non-opioid therapies were enrolled. Transdermal buprenorphine was ...
Jinno-Oue Atsushi - - 2012
Cell surface heparan sulfate proteoglycans (HSPGs) are involved in the binding and entry of human T-cell leukemia virus type 1 (HTLV-1) into host cells, while sulfated polysaccharides such as heparin inhibit HTLV-1 infection. Chondroitin sulfate proteoglycans (CSPGs) are classified as another major type of proteoglycans. Here, we examined the effect ...
Hellman Kevin M - - 2012
In anesthetized rats, opioid analgesia is accompanied by a specific pattern of tonic activity in two neuronal populations within the medullary raphe magnus (RM): opioids silence pain-facilitatory ON cells and produce sustained discharge in pain-inhibitory OFF cells. These tonic activity patterns, hypothesized to generate a tonic analgesic state, have not ...
Clyne Alisa M - - 2012
In this case study, an early-career mechanical engineer interviews an established translational bioscientist about mechanisms for merging engineering and biomedicine to pursue clinically informed research questions.
Maestre-Reyna Manuel - - 2012
The human pathogenic yeast Candida glabrata harbors more than 20 surface-exposed, epithelial adhesins (Epas) for host cell adhesion. The Epa family recognizes host glycans and discriminates between target tissues by their adhesin (A) domains, but a detailed structural basis for ligand-binding specificity of Epa proteins has been lacking so far. ...
Ku Manching - - 2012
ABSTRACT: BACKGROUND: The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure. RESULTS: We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly ...
Frierson Johnna M JM Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, - - 2012
Mammalian reoviruses display serotype-specific patterns of tropism and disease in the murine central nervous system (CNS) attributable to polymorphisms in viral attachment protein σ1. While all reovirus serotypes use junctional adhesion molecule-A as a cellular receptor, they differ in their utilization of carbohydrate coreceptors. This observation raises the possibility that ...
Kato Sayuri E - - 2012
To investigate further the contribution of the adenovirus type 5 (Ad5) E1B 55-kDa protein to genome replication, viral DNA accumulation was examined in primary human fibroblasts and epithelial cells infected with Ad5 or the E1B 55-kDa-null mutant Hr6. Unexpectedly, all cell types were observed to contain a significantly higher concentration ...
Kato Shiro - - 2012
Oenococcus oeni, a lactic acid bacterium, possesses a lysine racemase, which has a specific activity toward basic amino acids. A comparison of amino acid residues around the active site suggested that Ile222 and Tyr354 of the Geobacillus stearothermophilus alanine racemase, which shares 60% sequence similarity with lysine racemase, were replaced ...
Zhang Peng - - 2012
Hypoxia inducible factors 1-3 (HIF1-3) are transcription factors that regulate gene expression in response to hypoxia. Compared to our extensive understanding of HIF-1 and -2, our knowledge of HIF-3 is limited. In this study, we characterized the zebrafish hif-3α gene and determined its temporal and spatial expression, physiological regulation, and ...
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