Search Results
Results 1 - 50 of 3883
1 2 3 4 5 6 7 8 9 10 >
Festa Fernanda F Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ 85287-6401, - - 2013
In vitro transcription/translation (IVTT) systems are widely used in proteomics. For clinical applications, mammalian systems are preferred for protein folding and activity; however, the level of protein obtained is low. A new system extracted from human cells (1-Step Human Coupled IVT (HCIVT)) has the potential to overcome this problem and ...
O'Hegarty Michelle - - 2013
To determine prevalence of smoking, quit ratios, and home smoking rules among Hispanics residing in colonias in El Paso, Texas. Face-to-face interviews with 1485 Hispanic adults. GeoFrame™ field enumeration methods were used to develop a sampling frame from households in randomly selected colonias. The overall percent of current cigarette smoking ...
Motley Monica - - 2013
To qualitatively evaluate community capacity among an emerging coalition initiated to address complex regional obesity problems. Guided by dimensions of community capacity, this case study applies semistructured qualitative interviews among 12 key stakeholders engaged in the Dan River Partnership for a Healthy Community (DRPHC). In-depth qualitative data reveals important contextualized ...
Guthrie Lori C - - 2013
To examine associations between time perspective and smoking, body mass index, and exercise. In this community-based survey, adults reported smoking and exercise habits and weight and height and completed the Zimbardo Time Perspective Inventory. Subjects (N=265) who were more future oriented reported more frequent exercise, but were more likely to ...
Grzywacz Joseph G - - 2013
Determine the feasibility of using interactive voice response (IVR) for conducting daily diary research with immigrant Latinos. Baseline data containing demographic, health, and job-related characteristics were obtained from immigrant Latino construction workers (N=119). Participants also completed an IVR-based daily diary for 21 consecutive days. Over one third (37%) of the ...
Seo Dong-Chul - - 2013
To compare the prevalence and correlates of cigarette smoking among East Asian college students. Data were collected from college students (N=16,558) in China, Hong Kong, Malaysia, Singapore, South Korea, and Taiwan (response rate: 78%). Religion was independently associated with college students' smoking in China (adjusted odds ratio [AOR] = 1.82) ...
Greene Carolyn A - - 2013
To investigate the role of control beliefs in the relationship between SES and health. Two different aspects of perceived control - contingency beliefs (locus of control) and competence beliefs (self-efficacy) - were examined in relation to subjective SES and physical health outcomes (subjective health, functional impairment, chronic health problems, and ...
Kim Younkyoung - - 2013
To understand the predictors of controlled drinking in Korean male workers with a structural equation modeling based on Ajzen's theory of planned behavior. A cross-sectional survey design was used with a total of 315 male workers from 20 workplaces. The modified model accounted for 58% of intentions and 20% of ...
Gai Yunwei - - 2013
To examine factors associated with first-time use of preventive services based on the Behavioral Model of Health Services Use. Nine panels of the Medical Expenditure Panel Survey were merged to identify first-time users of 8 preventive services: blood pressure check, cholesterol screening, colonoscopy, flu vaccination, routine physical, Pap smear, mammogram, ...
Norman Gregory J - - 2013
To investigate whether self-selection moderated the effects of walkability on walking in overweight and obese men. 240 overweight and obese men completed measures on importance of walkability when choosing a neighborhood (selection) and preference for walkable features in general (preference). IPAQ measured walking. A walkbility index was derived from geographic ...
Simmelink Jennifer - - 2013
To explore East African refugees' perceptions, ideas, and beliefs about health and health care, as well as the ways in which health information is shared within their communities. This study consisted of 2 focus groups with a total of 15 participants, including East African community leaders and health professionals. East ...
Bolstad Anne L - - 2013
To examine the association of fruit and vegetable intake, acculturation, and BMI in Hispanics living in southern Nevada. Logistic regression was employed to assess the relationship of acculturation to daily intake of fruit and vegetables. Regression showed that greater acculturation (p = .002) and being male (p = .011) are ...
Woodhead Erin L - - 2013
To examine the associations between predisposing and need factors and receipt of mental health care and to examine age as a moderator of these associations. Participants (N=521) were surveyed as part of a larger study on health and adaptation. Obtaining mental health care was predicted by more reliance on approach ...
Speed-Andrews Amy E - - 2013
To estimate the prevalence and correlates of meeting the public health strength exercise guidelines (≥2 days/week) in colorectal cancer (CRC) survivors. A random sample of 600 CRC survivors in Alberta, Canada, completed a mailed questionnaire assessing medical, demographic, and behavioral variables and participation in strength exercise. About a quarter (25.5%) ...
Jun Jungmi - - 2013
To explore fatalistic attributions of colon cancer development among Asian and Hispanic Americans in comparison with non-Hispanic whites; also to examine the impacts of fatalism on adherence to the colon cancer screening guideline. For the analysis, the 2005 Health Information National Trends Survey data were employed. Both Asian and Hispanic ...
Miller Wayne C - - 2013
To document physical activity patterns and perceptions about active transport to school. Children's activity was measured. Children and parents were surveyed about active transport to school. Children were most active after (465 ± 20 cpm) and least active before (379 ± 18 cpm) and during school (351 ± 13 cpm). ...
DeVan Allison E - - 2013
In the present study, we tested the hypothesis that age-associated vascular endothelial dysfunction is exacerbated by IFG (impaired fasting plasma glucose) and that regular aerobic exercise prevents this effect. Data were analysed from a cohort of 131 non-smoking men and women without overt clinical disease. Compared with young adult controls ...
Baalman Kelli L KL Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, - - 2013
Exposure to a blast wave has been proposed to cause mild traumatic brain injury (mTBI), with symptoms including altered cognition, memory, and behavior. This idea, however, remains controversial, and the mechanisms of blast-induced brain injury remain unknown. To begin to resolve these questions, we constructed a simple compressed air shock ...
Pittman Roland N RN Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia, USA. - - 2013
Cells require energy to carry out their functions and they typically use oxidative phosphorylation to generate the needed ATP. Thus, cells have a continuous need for oxygen, which they receive by diffusion from the blood through the interstitial fluid. The circulatory system pumps oxygen-rich blood through a network of increasingly ...
Jonsson Catherine Aaro CA Murdoch Childrens Research Institute, Melbourne, - - 2013
Influence of childhood traumatic brain injury (TBI) on cognitive recovery and subsequent development is poorly understood. In this longitudinal study we used cluster analysis to explore acute stage individual profiles of injury age and cognition in 118 children with traumatic brain injury. Repeated measures of cognitive function were conducted at ...
Zhou Junmei J Central Laboratory, Children's Hospital of Shanghai, Shanghai Jiaotong University, Shanghai, - - 2013
Cryptorchidism is a common congenital birth defect in human beings with the possible complication of infertility. An in vitro model of cryptorchidism might be valuable due to the inaccessibility of human embryos for research purposes. In this study, we reprogrammed urine cells containing genetic variations in insulin-like factor 3, zinc ...
Chalupnikova Katerina - - 2013
Double-stranded RNA (dsRNA) is involved in different biological processes. At least three different pathways can respond to dsRNA in mammals. One of these pathways is RNA interference (RNAi) where long dsRNA induces sequence-specific degradation of transcripts carrying sequences complementary to dsRNA. Long dsRNA is also a potent trigger of the ...
Vaish Narendra - - 2013
The efficient and specific silencing of genes via RNA interference (RNAi) for functional genomics and therapeutics depends on careful consideration of the factors that affect the functionality of small interfering RNA (siRNA). These factors include (1) the length of sequence available for siRNA targeting of an mRNA, (2) the structural ...
Takasaki Shigeru - - 2013
Short interfering RNA (siRNA) has been widely used for studying gene function in mammalian cells but varies markedly in its gene silencing efficacy. Although many design rules/guidelines for effective siRNAs based on various criteria have been reported recently, there are only a few consistencies among them. This makes it difficult ...
Ranftler Carmen - - 2013
Detailed insight into the fine structure and 3D-architecture of the complex and dynamic compartments of the endocytic system is essential for a morpho-functional analysis of retrograde traffic from the cell surface to different intracellular destinations. Here, we describe a cytochemical approach for electron microscopic exploration of endocytic pathways with the ...
Rosso Francesco - - 2013
Immunogold labeling (IGL) technique has been utilized by many authors in combination with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to obtain the identification/localization of receptors and antigens, both in cells and tissues. Environmental scanning electron microscopy (ESEM) represents an important tool in biomedical research, since it does ...
Quinn Anthony S - - 2013
The information covered in this chapter will present a model homogenous membrane preparation technique and dynamic imaging procedure that can be successfully applied to more than one type of lipid study and atomic force microscope (AFM) instrument setup. The basic procedural steps have been used with an Asylum Research MFP-3D ...
Adler Jeremy - - 2013
The measurement of colocalization requires images of two fluorophores that are aligned, with no cross talk, and that the intensities remain within the response range of the microscope. Quantitation depends upon differentiating between the presence and absence of fluorescence, and measurements should be made within biologically relevant regions of interest. ...
Bramsen Jesper B - - 2013
Synthetic small interfering RNAs (siRNAs) have revolutionized functional genomics in mammalian cell cultures due to their reliability, efficiency, and ease of use. This success, however, has not fully translated into siRNA applications in vivo and in siRNA therapeutics where initial optimism has been dampened by a lack of efficient delivery ...
Vonturkovich Michele A - - 2013
Nerve Morphometry is one tool employed in the clinical assessment of peripheral sural nerve pathological abnormalities. A new method is presented in this chapter incorporating an unbiased approach to quantitative sural nerve evaluation. Using conventional epoxy embedded nerves processed for electron microscopy, confocal microscopy, and interactive digital assessment, this method ...
Fahim Muhammad - - 2013
RNA-mediated virus resistance is increasingly becoming a method of choice for antiviral defense in plants when effective natural resistance is unavailable. In this chapter we discuss the design principles of artificial micro RNA (amiRNA), in which a natural miRNA precursor gene is modified to target a different species of RNA, ...
Meisslitzer-Ruppitsch Claudia - - 2013
Correlative microscopic approaches combine the advantages of both light and electron microscopy. Here we show a correlative approach that uses the photooxidation capacity of fluorescent dyes. Through illumination with high energetic light, the chromogen diaminobenzidine is oxidized and stable deposits are formed at the sites of the former fluorescent signals, ...
Liu Ying Poi - - 2013
RNA interference (RNAi) has been widely used as a tool for gene knockdown in fundamental research and for the development of new RNA-based therapeutics. The RNAi pathway is typically induced by expression of ∼22 base pair (bp) small interfering RNAs (siRNAs), which can be transfected into cells. For long-term gene ...
Felippes Felipe Fenselau de - - 2013
In plants, some microRNAs (miRNAs) can trigger the production of secondary small interfering RNAs (siRNAs) from their targets. miRNA-induced gene silencing (MIGS) exploits this unique feature to efficiently downregulate gene expression. The simple flanking of a sequence of interest with the target site for the miR173 (an miRNA able to ...
Evensen Lasse - - 2013
Automated multicolor fluorescence microscopy facilitates high-throughput quantitation of cellular parameters of complex, organotypic systems. In vitro co-cultured vascular cells form capillary-like networks that model facets of angiogenesis, making it an attractive alternative for anti-angiogenic drug discovery. We have adapted this angiogenesis assay system to a high-throughput format to enable automated ...
Tomer Raju - - 2013
Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications.Here, ...
Jena Bhanu P - - 2013
Cells synthesize and store within membranous sacs products such as hormones, growth factors, neurotransmitters, or digestive enzymes, for release on demand. As recently as just 15 years ago, it was believed that during cell secretion, membrane-bound secretory vesicles completely merge at the cell plasma membrane resulting in the diffusion of ...
Matveeva Olga - - 2013
The design of small gene silencing RNAs with a high probability of being efficient still has some elements of an art, especially when the lowest concentration of small molecules needs to be utilized. The design of highly target-specific small interfering RNAs or short hairpin RNAs is even a greater challenging ...
Nasheri Neda - - 2013
RNA silencing is an invaluable tool to interrogate gene function. The cytoplasmic delivery of small interfering RNAs (siRNAs) complementary to a gene of interest results in cleavage and degradation of the target mRNA. Given the potential to target virtually any RNA, siRNA-based therapeutics may revolutionize the treatment of disease. Target ...
Tabata Keisuke - - 2013
Autophagy is a bulk intracellular degradation process that is ubiquitous in eukaryotic cells and helps to recycle nutrients from catabolites by degrading proteins, lipids, and glycans, including organelles. Since autophagy has divergent physiological roles in cancer, infection, immunity, and other processes, it is important to accurately analyze autophagic activity. In ...
Gantier Michael P - - 2013
Specific clinical applications of RNA interference (RNAi) can benefit from a concurrent activation of the immune system. This is the case for small interfering RNAs (siRNAs) with antitumor or antiviral activities. This chapter provides a brief overview of the strategies reported to date to design siRNAs with gene silencing and ...
Walther Paul - - 2013
Using an electron microscope's scanning transmission mode (STEM) for collection of tomographic datasets is advantageous compared to bright field transmission electron microscopic (TEM). For image formation, inelastic scattering does not cause chromatic aberration, since in STEM mode no image forming lenses are used after the beam has passed the sample, ...
Chang Chanil - - 2013
RNA interference (RNAi) is a highly efficient endogenous gene silencing mechanism mediated by short double-stranded RNAs termed small interfering RNAs (siRNAs). The current standard siRNA structure, which is used by most researchers to trigger sequence-specific target gene silencing, consists of a double strand region of 19 bp with 2 nt ...
Sætrom Pål - - 2013
Short interfering RNAs (siRNAs) have become valued tools for knocking down specific genes. As such, siRNAs are routinely used to study gene function and are also being explored as therapeutic agents. Traditionally, siRNAs are designed to target one specific gene, but this chapter describes a procedure for designing dual-targeting siRNAs ...
Lacoste J - - 2013
Fluorescence microscopy has revolutionized the way live-cell imaging is achieved. At the same time, it is also potentially harmful to a living specimen. Therefore, the specimen must be monitored for viability and health before, during, and after imaging sessions. Methods for monitoring cell viability and health will be discussed in ...
Suzuki Michitaka - - 2013
Cells store excess lipid as esters in the form of triglycerides and cholesterol esters. Most lipid esters are compartmentalized in globular structures called lipid droplets. Here we describe several methods of detecting lipid droplets by fluorescence microscopy. Lipid droplets can be visualized either by staining the lipid ester core using ...
Shankaran Sunita S - - 2013
Real-time imaging coupled with a permeabilized cell system presents a very versatile platform to visualize the dynamic and intricate nature of nuclear envelope breakdown, one of the major morphological changes of mitosis. Here, we describe such a strategy in which the plasma membrane of cells expressing fluorescently tagged nucleoporin POM121 ...
Chtcheglova Lilia A - - 2013
One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy ...
Polishchuk Elena V - - 2013
Correlative light-electron microscopy (CLEM) is a very effective technique that combines live-cell imaging and immuno-electron microscopy for ultrastructural morphological characterization of dynamic intracellular organelles. The use of green fluorescent protein (GFP)-tagged chimeras allows the user to follow the movements and/or behavior of intracellular structures in a live cell and to ...
Mackay Joanna L - - 2013
Atomic force microscopy (AFM) is a powerful and versatile tool for probing the mechanical properties of biological samples. This chapter describes the procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope ...
1 2 3 4 5 6 7 8 9 10 >