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Wang Jie J Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ 85287-6401, - - 2013
We aim to develop a protein microarray platform capable of presenting both natural and denatured forms of proteins for antibody biomarker discovery. We will further optimize plasma screening protocols to improve detection. We developed a new covalent capture protein microarray chemistry using HaloTag fusion proteins and ligand. To enhance protein ...
Festa Fernanda F Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ 85287-6401, - - 2013
In vitro transcription/translation (IVTT) systems are widely used in proteomics. For clinical applications, mammalian systems are preferred for protein folding and activity; however, the level of protein obtained is low. A new system extracted from human cells (1-Step Human Coupled IVT (HCIVT)) has the potential to overcome this problem and ...
Gai Yunwei - - 2013
To examine factors associated with first-time use of preventive services based on the Behavioral Model of Health Services Use. Nine panels of the Medical Expenditure Panel Survey were merged to identify first-time users of 8 preventive services: blood pressure check, cholesterol screening, colonoscopy, flu vaccination, routine physical, Pap smear, mammogram, ...
Guthrie Lori C LC National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, - - 2013
To examine associations between time perspective and smoking, body mass index, and exercise. In this community-based survey, adults reported smoking and exercise habits and weight and height and completed the Zimbardo Time Perspective Inventory. Subjects (N=265) who were more future oriented reported more frequent exercise, but were more likely to ...
Greene Carolyn A - - 2013
To investigate the role of control beliefs in the relationship between SES and health. Two different aspects of perceived control - contingency beliefs (locus of control) and competence beliefs (self-efficacy) - were examined in relation to subjective SES and physical health outcomes (subjective health, functional impairment, chronic health problems, and ...
Kim Younkyoung - - 2013
To understand the predictors of controlled drinking in Korean male workers with a structural equation modeling based on Ajzen's theory of planned behavior. A cross-sectional survey design was used with a total of 315 male workers from 20 workplaces. The modified model accounted for 58% of intentions and 20% of ...
Bolstad Anne L - - 2013
To examine the association of fruit and vegetable intake, acculturation, and BMI in Hispanics living in southern Nevada. Logistic regression was employed to assess the relationship of acculturation to daily intake of fruit and vegetables. Regression showed that greater acculturation (p = .002) and being male (p = .011) are ...
Woodhead Erin L - - 2013
To examine the associations between predisposing and need factors and receipt of mental health care and to examine age as a moderator of these associations. Participants (N=521) were surveyed as part of a larger study on health and adaptation. Obtaining mental health care was predicted by more reliance on approach ...
DeVan Allison E - - 2013
In the present study, we tested the hypothesis that age-associated vascular endothelial dysfunction is exacerbated by IFG (impaired fasting plasma glucose) and that regular aerobic exercise prevents this effect. Data were analysed from a cohort of 131 non-smoking men and women without overt clinical disease. Compared with young adult controls ...
Miller Wayne C - - 2013
To document physical activity patterns and perceptions about active transport to school. Children's activity was measured. Children and parents were surveyed about active transport to school. Children were most active after (465 ± 20 cpm) and least active before (379 ± 18 cpm) and during school (351 ± 13 cpm). ...
Grzywacz Joseph G - - 2013
Determine the feasibility of using interactive voice response (IVR) for conducting daily diary research with immigrant Latinos. Baseline data containing demographic, health, and job-related characteristics were obtained from immigrant Latino construction workers (N=119). Participants also completed an IVR-based daily diary for 21 consecutive days. Over one third (37%) of the ...
Norman Gregory J - - 2013
To investigate whether self-selection moderated the effects of walkability on walking in overweight and obese men. 240 overweight and obese men completed measures on importance of walkability when choosing a neighborhood (selection) and preference for walkable features in general (preference). IPAQ measured walking. A walkbility index was derived from geographic ...
Motley Monica - - 2013
To qualitatively evaluate community capacity among an emerging coalition initiated to address complex regional obesity problems. Guided by dimensions of community capacity, this case study applies semistructured qualitative interviews among 12 key stakeholders engaged in the Dan River Partnership for a Healthy Community (DRPHC). In-depth qualitative data reveals important contextualized ...
Seo Dong-Chul - - 2013
To compare the prevalence and correlates of cigarette smoking among East Asian college students. Data were collected from college students (N=16,558) in China, Hong Kong, Malaysia, Singapore, South Korea, and Taiwan (response rate: 78%). Religion was independently associated with college students' smoking in China (adjusted odds ratio [AOR] = 1.82) ...
Simmelink Jennifer - - 2013
To explore East African refugees' perceptions, ideas, and beliefs about health and health care, as well as the ways in which health information is shared within their communities. This study consisted of 2 focus groups with a total of 15 participants, including East African community leaders and health professionals. East ...
O'Hegarty Michelle - - 2013
To determine prevalence of smoking, quit ratios, and home smoking rules among Hispanics residing in colonias in El Paso, Texas. Face-to-face interviews with 1485 Hispanic adults. GeoFrame™ field enumeration methods were used to develop a sampling frame from households in randomly selected colonias. The overall percent of current cigarette smoking ...
Jun Jungmi - - 2013
To explore fatalistic attributions of colon cancer development among Asian and Hispanic Americans in comparison with non-Hispanic whites; also to examine the impacts of fatalism on adherence to the colon cancer screening guideline. For the analysis, the 2005 Health Information National Trends Survey data were employed. Both Asian and Hispanic ...
Speed-Andrews Amy E - - 2013
To estimate the prevalence and correlates of meeting the public health strength exercise guidelines (≥2 days/week) in colorectal cancer (CRC) survivors. A random sample of 600 CRC survivors in Alberta, Canada, completed a mailed questionnaire assessing medical, demographic, and behavioral variables and participation in strength exercise. About a quarter (25.5%) ...
Baalman Kelli L KL Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, - - 2013
Exposure to a blast wave has been proposed to cause mild traumatic brain injury (mTBI), with symptoms including altered cognition, memory, and behavior. This idea, however, remains controversial, and the mechanisms of blast-induced brain injury remain unknown. To begin to resolve these questions, we constructed a simple compressed air shock ...
Pittman Roland N RN Department of Physiology and Biophysics, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia, USA. - - 2013
Cells require energy to carry out their functions and they typically use oxidative phosphorylation to generate the needed ATP. Thus, cells have a continuous need for oxygen, which they receive by diffusion from the blood through the interstitial fluid. The circulatory system pumps oxygen-rich blood through a network of increasingly ...
Jonsson Catherine Aaro CA Murdoch Childrens Research Institute, Melbourne, - - 2013
Influence of childhood traumatic brain injury (TBI) on cognitive recovery and subsequent development is poorly understood. In this longitudinal study we used cluster analysis to explore acute stage individual profiles of injury age and cognition in 118 children with traumatic brain injury. Repeated measures of cognitive function were conducted at ...
Zhou Junmei J Central Laboratory, Children's Hospital of Shanghai, Shanghai Jiaotong University, Shanghai, - - 2013
Cryptorchidism is a common congenital birth defect in human beings with the possible complication of infertility. An in vitro model of cryptorchidism might be valuable due to the inaccessibility of human embryos for research purposes. In this study, we reprogrammed urine cells containing genetic variations in insulin-like factor 3, zinc ...
Sun Wei - - 2013
Optical microscopy is a simple yet robust strategy to study live cellular processes. By changing the wavelength of the illumination light, different non-fluorescent nanoparticle probes can be identified and tracked dynamically inside crowded living cells with either differential interference contrast (DIC) microscopy or planar illumination microscopy (PIM). The translational and ...
Chang Chanil - - 2013
RNA interference (RNAi) is a highly efficient endogenous gene silencing mechanism mediated by short double-stranded RNAs termed small interfering RNAs (siRNAs). The current standard siRNA structure, which is used by most researchers to trigger sequence-specific target gene silencing, consists of a double strand region of 19 bp with 2 nt ...
Rosso Francesco - - 2013
Immunogold labeling (IGL) technique has been utilized by many authors in combination with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to obtain the identification/localization of receptors and antigens, both in cells and tissues. Environmental scanning electron microscopy (ESEM) represents an important tool in biomedical research, since it does ...
Ranftler Carmen - - 2013
Detailed insight into the fine structure and 3D-architecture of the complex and dynamic compartments of the endocytic system is essential for a morpho-functional analysis of retrograde traffic from the cell surface to different intracellular destinations. Here, we describe a cytochemical approach for electron microscopic exploration of endocytic pathways with the ...
Felippes Felipe Fenselau de - - 2013
In plants, some microRNAs (miRNAs) can trigger the production of secondary small interfering RNAs (siRNAs) from their targets. miRNA-induced gene silencing (MIGS) exploits this unique feature to efficiently downregulate gene expression. The simple flanking of a sequence of interest with the target site for the miR173 (an miRNA able to ...
Rao Donald D - - 2013
The discovery of RNA interference (RNAi) engendered great excitement and raised expectations regarding its potential applications in biomedical research and clinical usage. Over the ensuing years, expanded understanding of RNAi and preliminary results from early clinical trials tempered enthusiasm with realistic appraisal resulting in cautious optimism and a better understanding ...
Dallas Anne - - 2013
Synthetic shRNAs that are too short to be Dicer substrates (short shRNAs or sshRNAs) can be highly potent RNAi effectors when properly designed, with activities similar to or more potent than the more commonly used siRNAs targeting the same sequences. sshRNAs can be designed in two possible orientations: left- or ...
Surendranath Vineeth - - 2013
RNA interference (RNAi) has grown to be one of the main techniques for loss-of-function studies, leading to the elucidation of biological function of genes in various cellular systems and model organisms. While for many invertebrates such as Drosophila melanogaster (D. melanogaster) and Caenorhabditis elegans (C. elegans) long double-stranded RNA (dsRNA) ...
Shlyakhtenko Luda S LS Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, - - 2013
Surface preparation is a key step for reliable and reproducible imaging of DNA and protein-DNA complexes with atomic force microscopy (AFM). This article describes the approaches for chemical functionalization of the mica surface. One approach utilizes 3-aminopropyl-trietoxy silane (APTES), enabling one to obtain a smooth surface termed AP-mica. This surface ...
Chtcheglova Lilia A - - 2013
One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy ...
Chalupnikova Katerina - - 2013
Double-stranded RNA (dsRNA) is involved in different biological processes. At least three different pathways can respond to dsRNA in mammals. One of these pathways is RNA interference (RNAi) where long dsRNA induces sequence-specific degradation of transcripts carrying sequences complementary to dsRNA. Long dsRNA is also a potent trigger of the ...
Nasheri Neda - - 2013
RNA silencing is an invaluable tool to interrogate gene function. The cytoplasmic delivery of small interfering RNAs (siRNAs) complementary to a gene of interest results in cleavage and degradation of the target mRNA. Given the potential to target virtually any RNA, siRNA-based therapeutics may revolutionize the treatment of disease. Target ...
Walther Paul - - 2013
Using an electron microscope's scanning transmission mode (STEM) for collection of tomographic datasets is advantageous compared to bright field transmission electron microscopic (TEM). For image formation, inelastic scattering does not cause chromatic aberration, since in STEM mode no image forming lenses are used after the beam has passed the sample, ...
Tanaka Hiromitsu - - 2013
Transfection of short hairpin RNA (shRNA) expression plasmids is conventionally performed for gene-specific knockdown in cultured mammalian and insect cells. Here, I describe a simple method to synthesize an inverted repeat DNA in a U6 small nuclear RNA promoter-based parent vector using a single-stranded inverted repeat DNA and Bst DNA ...
Lacoste J - - 2013
Fluorescence microscopy has revolutionized the way live-cell imaging is achieved. At the same time, it is also potentially harmful to a living specimen. Therefore, the specimen must be monitored for viability and health before, during, and after imaging sessions. Methods for monitoring cell viability and health will be discussed in ...
Tabata Keisuke - - 2013
Autophagy is a bulk intracellular degradation process that is ubiquitous in eukaryotic cells and helps to recycle nutrients from catabolites by degrading proteins, lipids, and glycans, including organelles. Since autophagy has divergent physiological roles in cancer, infection, immunity, and other processes, it is important to accurately analyze autophagic activity. In ...
Cromey Douglas W DW Department of Cellular and Molecular Medicine, Southwest Environmental Health Sciences Center, Arizona Research Labs-Division of Biotechnology, Arizona Cancer Center, University of Arizona College of Medicine, Tuscon, AZ, USA. - - 2013
The scientific community has become very concerned about inappropriate image manipulation. In journals that check figures after acceptance, 20-25% of the papers contained at least one figure that did not comply with the journal's instructions to authors. The scientific press continues to report a small, but steady stream of cases ...
Gantier Michael P - - 2013
Specific clinical applications of RNA interference (RNAi) can benefit from a concurrent activation of the immune system. This is the case for small interfering RNAs (siRNAs) with antitumor or antiviral activities. This chapter provides a brief overview of the strategies reported to date to design siRNAs with gene silencing and ...
Polishchuk Elena V - - 2013
Correlative light-electron microscopy (CLEM) is a very effective technique that combines live-cell imaging and immuno-electron microscopy for ultrastructural morphological characterization of dynamic intracellular organelles. The use of green fluorescent protein (GFP)-tagged chimeras allows the user to follow the movements and/or behavior of intracellular structures in a live cell and to ...
Naito Yuki - - 2013
RNA interference (RNAi) mediated by small interfering RNA (siRNA) is now widely used to knock down gene expression in a sequence-specific manner, making it a powerful tool not only for studying gene functions but also for therapeutic applications. siRNA decreases the expression level of the intended target gene with complete ...
Pozarowski Piotr P The Brander Cancer Research Institute, New York Medical College, Valhalla, NY, - - 2013
Laser scanning cytometer (LSC) is the microscope-based cytofluorometer that offers a plethora of unique analytical capabilities, not provided by flow cytometry (FCM). This review describes attributes of LSC and covers its numerous applications derived from plentitude of the parameters that can be measured. Among many LSC applications the following are ...
Chernolovskaya Elena L - - 2013
Small interfering RNAs (siRNAs) are potent inducers of RNA interference-the conservative cellular process of posttranscriptional gene silencing. The silencing activity of siRNAs depends on the thermodynamic asymmetry of the siRNA duplex. Here, we describe the design of chemically modified fork-like siRNA (fsiRNAs) containing mismatches at the 3'-end region of the ...
Quinn Anthony S - - 2013
The information covered in this chapter will present a model homogenous membrane preparation technique and dynamic imaging procedure that can be successfully applied to more than one type of lipid study and atomic force microscope (AFM) instrument setup. The basic procedural steps have been used with an Asylum Research MFP-3D ...
Meding Stephan - - 2013
MALDI (Matrix-Assisted Laser Desorption/Ionization) Imaging mass spectrometry is a powerful new method for analyzing the spatial distribution of molecules in tissues. Several different classes of cellular constituents such as proteins, peptides, lipids, and small molecules can be analyzed in situ while maintaining the morphological integrity of the tissue. This allows ...
Webb Donna J DJ Department of Biological Sciences, Vanderbilt University, Nashville, TN, - - 2013
Epi-fluorescence microscopy is available in most life sciences research laboratories, and when optimized can be a central laboratory tool. In this chapter, the epi-fluorescence light path is introduced and the various components are discussed in detail. Recommendations are made for incident lamp light sources, excitation and emission filters, dichroic mirrors, ...
Masedunskas Andrius A Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, - - 2013
Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or ...
Horn Thomas - - 2013
RNAi has become a very versatile tool to silence gene expression in a variety of organisms, in particular when classical genetic methods are missing. However, the application of this method in functional studies has raised new challenges in order to design RNAi reagents that minimize false positives and false negatives. ...
Jena Bhanu P - - 2013
Cells synthesize and store within membranous sacs products such as hormones, growth factors, neurotransmitters, or digestive enzymes, for release on demand. As recently as just 15 years ago, it was believed that during cell secretion, membrane-bound secretory vesicles completely merge at the cell plasma membrane resulting in the diffusion of ...
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