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Kung Justin W - - 2012
Digital professionalism is increasingly being integrated into postgraduate medical education. We developed a small-group, reflective practice-based session incorporating radiology-specific cases to heighten residents' awareness about digital professionalism. Case-based, radiology-specific scenarios were created for a small-group, reflective practice-based session on digital professionalism. Anonymous pre- and postsession surveys evaluating residents' use of ...
Francis W R - - 2012
Changes in human bone marrow associated with the systemic inflammatory response to injury are little understood. It was hypothesized that major trauma results in an altered bone marrow leucocyte progenitor profile, with either uniform depletion or the balance between multipotent and committed progenitors varying, depending on whether self-renewal is favoured ...
Stremitzer S - - 2012
The prognostic value of KRAS mutation in patients with colorectal cancer liver metastases (CLM) receiving neoadjuvant chemotherapy including bevacizumab before liver resection is unclear. The KRAS and BRAF status of resected CLM was assessed in prospectively studied patients. Mutations were correlated with recurrence-free and overall survival. Only patients with remaining ...
Kahraman Deniz - - 2012
The aim was to assess the value of tumor lesion glycolysis (TLG) and tumor lesion proliferation (TLP) determined by FDG and 3'-deoxy-3'-F-fluorothymidine (FLT) PET for response prediction and prognostic differentiation in patients with advanced non-small cell lung cancer (NSCLC) treated with erlotinib. FDG-PET and FLT-PET were performed in 30 patients ...
Leventhal Adam M - - 2012
To examine the relation of 2 measures of anhedonia-a specific facet of depression indicative of inability to experience pleasure-to physical activity (PA). Cross-sectional correlational survey study of 157 college students (73% female, M age 19.9 years). One or both measures of anhedonia were inversely associated with walking frequency, moderate-intensity PA ...
Kelly Kimberly M - - 2012
To examine perceptions of cervical cancer risk in elevated-risk Appalachians. Appalachian women (n=571) completed interviews examining self-regulation model factors relevant to perceived risk of cervical cancer. Women with good/very good knowledge of cervical cancer, greater worry, and history of sexually transmitted infection had higher odds of rating their perceived risk ...
Hock Barry D - - 2012
Human polymorphonuclear leucocytes (PMN) are thought to be immunosuppressive. The suppressive mechanism(s) used by PMN are, however, not well defined and in this study they were analysed using T-cell responses to CD3(+) CD28 monoclonal antibodies (mAb) as a readout. We demonstrate that in vitro activated PMN (PMN(act)) can, without any ...
Weigand Luise U - - 2012
Adoptive transfer of T cells genetically modified with tumour-specific T-cell receptors (TCR) is a promising novel approach in the treatment of cancer. We have previously isolated an allorestricted MHC class I-restricted TCR with specificity for Formin-like protein 1 (FMNL1) with potent activity against chronic lymphocytic leukaemia cells. CD4(+) T cells ...
Escoto Kamisha Hamilton - - 2012
To describe time-related beliefs and behaviors regarding healthful eating, indicators of dietary intake, and their associations with the number of weekly hours of paid work among young adults. Population-based study in a diverse cohort (N=2287). Working > 40 hours per week was associated with time-related barriers to healthful eating most ...
Daley Ellen M EM Department of Community and Family Health, University of South Florida, College of Public Health, Tampa, FL, USA. - - 2012
To report psychosocial differences between self-reported HPV-positive and HPV-negative Florida men. Men (n=536) in an HPV natural-history study completed a cross-sectional survey (2007-2010). Most participants were white, non-Hispanic, and unmarried with some college education. HPV knowledge was high overall. HPV-positive men reported more negative emotions and perceived threat (P<0.05). HPV-negative ...
Herholz Karl K School of Cancer and Enabling Sciences, The University of Manchester, Wolfson Molecular Imaging Centre, Manchester, England. - - 2012
This review addresses the specific contributions of nuclear medicine techniques, and especially positron emission tomography (PET), for diagnosis and management of brain tumors. (18)F-Fluorodeoxyglucose PET has particular strengths in predicting prognosis and differentiating cerebral lymphoma from nonmalignant lesions. Amino acid tracers including (11)C-methionine, (18)F-fluoroethyltyrosine, and (18)F-L-3,4-dihydroxyphenylalanine provide high sensitivity, which ...
Mayes Maureen D MD Division of Rheumatology and Clinical Immunogenetics, University of Texas Health Science Center, Houston, Texas 77030, USA. - - 2012
The last decade has seen enormous progress in understanding genetic associations of systemic sclerosis to explain the observed heritability. This review highlights the most recent findings and places them in the context of proposed functional roles. Over 30 genes and gene regions have now been identified as scleroderma susceptibility loci. ...
Mathis Chester A CA Department of Radiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. - - 2012
For 100 years, β-amyloid (Aβ) plaques and neurofibrillary tangles (NFTs) have been recognized as the neuropathological hallmarks of Alzheimer's disease (AD), and their presence or absence could only be assessed postmortem using stains and dyes that identified these microscopic structures. Approximately 10 years ago, the first successful Aβ plaque-specific positron ...
Rainwater-Lovett Kaitlin K Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, - - 2013
To explore immunologic risk factors for death within 90 days of highly active antiretroviral therapy (HAART) initiation, CD4(+) and CD8(+) T cell subsets were measured by flow cytometry and characterized by logistic regression in 149 Zambian children between 9 months and 10 years of age enrolled in a prospective, observational ...
Johnson Ruth I RI Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA. - - 2012
Morphogenetic modeling of tissues requires coordinated regulation of adhesion. For its correct patterning, the Drosophila pupal eye requires several Immunoglobulin superfamily cell adhesion molecules (IgCAMs) and the adaptor protein Cindr. Orthologs of these proteins are essential components of specialized junctions of the vertebrate kidney; the Cindr ortholog Cd2ap is essential ...
Brown Anthony A Department of Neuroscience, The Ohio State University, Columbus, OH 43210, USA. - - 2013
Neurofilaments are transported along axons in a rapid intermittent and bidirectional manner but there is a long-standing controversy about whether this applies to all axonal neurofilaments. Some have proposed that only a small proportion of axonal neurofilaments are mobile and that most are deposited into a persistently stationary and extensively ...
Pastoret Cedric - - 2013
The Kleihauer-Betke test (KBT) is the most widely used assay for fetomaternal hemorrhage (FMH) detection in rhesus D negative women. In the current study, we sought to evaluate the performance of a flow cytometry (FCM) kit (FMH QuikQuant) using an anti-HbF antibody. Eighty-three pregnant women, 58 umbilical cord blood (UCB) ...
Clément Aurélie - - 2012
Cyclin-dependent kinases (Cdks) and their counteracting phosphatases are key regulators of cell cycle progression. In yeasts, the Cdc14 family of phosphatases promotes exit from mitosis and progression through cytokinesis by reversing phosphorylation of Cdk1 substrates. In vertebrates, CDC14 paralogs, CDC14A and CDC14B, have so far been implicated in processes ranging ...
Schaper J Niklas JN Institute of Inorganic and Analytical Chemistry, University of Mainz, 55128 Mainz, - - 2012
One of the fastest developing fields in analytical spectrochemistry in recent years is ambient desorption/ionization mass spectrometry (ADI-MS). This burgeoning interest has been due to the demonstrated advantages of the method: simple mass spectra, little or no sample preparation, and applicability to samples in the solid, liquid, or gaseous state. ...
Ichimura Koichiro K Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104, - - 2012
The podocyte slit diaphragm (SD) is an essential component of the selective filtration barrier in the glomerulus. Several structural proteins required for formation and maintenance of SD have been identified; however, molecular mechanisms regulating these proteins are still limited. Here, we demonstrate that MAGUK p55 subfamily member 5a (Mpp5a)/Nagie oko, ...
Erickson Blake B Program in Biophysics, University of Michigan, Ann Arbor, MI 48109-1055, - - 2013
This article details a quantitative method to measure the D-periodic spacing of type I collagen fibrils using atomic force microscopy coupled with analysis using a two-dimensional fast fourier transform approach. Instrument calibration, data sampling and data analysis are discussed and comparisons of the data to the complementary methods of electron ...
Ma Liang - - 2012
Nonalcoholic fatty liver disease (NAFLD), one of chronic liver diseases, seems to be rising as the obesity epidemic continues. In this study, 54 novel (thio)barbituric acid derivatives have been synthesized and evaluated for pharmacological activity. 7h exhibited potent glucose-lowering effects on insulin-resistant HepG2 cells and regulated adiponectin and leptin expression ...
Ornelas-Megiatto Cátia - - 2012
Degradable acetalated dextran (Ac-DEX) nanoparticles were prepared and loaded with a hydrophobic silver carbene complex (SCC) by a single-emulsion process. The resulting particles were characterized for morphology and size distribution using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The average particle size and particle ...
Venable John D JD Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United - - 2012
Amide hydrogen/deuterium exchange is a commonly used technique for studying the dynamics of proteins and their interactions with other proteins or ligands. When coupled with liquid chromatography and mass spectrometry, hydrogen/deuterium exchange provides several unique advantages over other structural characterization techniques including very high sensitivity, the ability to analyze proteins ...
Lasonder Edwin E Centre for Molecular and Biomolecular Informatics, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands. - - 2012
The asexual blood stages of Plasmodium falciparum cause the most lethal form of human malaria. During growth within an infected red blood cell, parasite multiplication and formation of invasive merozoites is called schizogony. Here, we present a detailed analysis of the phosphoproteome of P. falciparum schizonts revealing 2541 unique phosphorylation ...
Marginean Ioan I Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, United - - 2012
Human serum glycan profiling with mass spectrometry (MS) has been employed to study several disease conditions and is demonstrating promise in, for example, clinical biomarker discovery. However, the low glycan ionization efficiency and the large dynamic range of glycan concentrations in human sera can hinder comprehensive profiling. In particular, large ...
Wang Feng F NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43212, United - - 2012
A novel and facile approach has been developed to create thermoresponsive surfaces with macroscale patterns together with microscale features. The surface patterns were formed by applying macroscale nucleation agent patterns onto saturated N-isopropylacrylamide monomer solution membranes to induce the divergent growth of needlelike monomer crystals; the patterned monomer crystals were ...
Fuhs Thomas T Department of Physics, Soft Matter Physics Division, University of Leipzig, Linnéstr. 5, 04103 Leipzig, Germany. - - 2013
Previous results have shown that glial cells provide a soft environment for the neurons of the mammalian central nervous system (CNS). This raises the question whether neurons are confined to the CNS and cannot wander off into more rigid tissues, such as brain capillary walls. We investigated the mechanical properties ...
Maia Ana Rita Ramada AR Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 465 21st Avenue South, Nashville, TN 37232, - - 2013
A microtubule (MT) subpopulation that emanates from Golgi membrane has been recently shown to comprise a significant part of MT network in interphase cells. In this study, we address whether Golgi membrane, which is being extensively remodeled throughout the cell cycle, retains its ability to nucleate MTs at diverse cell ...
Portran Didier D Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherches en Technologies et Sciences pour le Vivant, UMR5168, CNRS/CEA/INRA/Université Joseph Fourier, Grenoble, - - 2013
The spatial organization of the microtubule (MT) network directs cell polarity and mitosis. It is finely regulated by hundreds of different types of microtubule-associated proteins and molecular motors whose specific functions are difficult to investigate directly in cells. Here, we have investigated their functions using geometrically controlled MT networks in ...
Balasubramanian Mohan K MK Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604. - - 2012
Cytokinesis is the final stage of the cell cycle during which a cell physically divides into two daughters through the assembly of new membranes (and cell wall in some cases) between the forming daughters. New membrane assembly can either proceed centripetally behind a contractile apparatus, as in the case of ...
Bassett G Randall - - 2012
Apolipoprotein (apo) A-II, the second most abundant protein after apo A-I of human plasma high-density lipoproteins (HDL), is the most lipophilic of the exchangeable apolipoproteins. The rate of microsolubilization of dimyristoylphosphatidylcholine (DMPC) membranes by apo A-I to give rHDL increases as the level of membrane free cholesterol (FC) increases up ...
De Soumya - - 2012
Peptidyl prolyl cis-trans isomerization acts as an effective molecular timer that plays significant roles in biological and pathological processes. Enzymes such as Pin1 catalyze cis-trans isomerization, accelerating the otherwise slow isomerization rate into time scales relevant for cellular signaling. Here we have combined NMR line shape analysis, fluorescence spectroscopy, and ...
Dasuri Kalavathi K Pennington Biomedical Research Center , Louisiana State University System, Baton Rouge, LA 70808, - - 2013
Lipid peroxidation products such as 4-hydroxynonenal (HNE) are known to be increased in response to oxidative stress, and are known to cause dysfunction and pathology in a variety of tissues during periods of oxidative stress. The aim of the current study was to determine the chronic (repeated HNE exposure) and ...
Blakeley Paul - - 2012
Proteogenomics has the potential to advance genome annotation through high quality peptide identifications derived from mass spectrometry experiments, which demonstrate a given gene or isoform is expressed and translated at the protein level. This can advance our understanding of genome function, discovering novel genes and gene structure that have not ...
Das Rajat S RS Department of Chemistry, University of Connecticut, Storrs, CT 06269, - - 2012
8,5'-Cyclopurine deoxynucleosides are unique tandem lesions containing an additional covalent bond between the base and the sugar. These mutagenic and genotoxic lesions are repaired only by nucleotide excision repair. The N-glycosidic (or C1'-N9) bond of 2'-deoxyguanosine (dG) derivatives is usually susceptible to acid hydrolysis, but even after cleavage of this ...
Bowden Thomas A TA Division of Structural Biology, University of Oxford, Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. - - 2012
Human IgG Fc glycosylation modulates immunological effector functions such as antibody-dependent cellular cytotoxicity and phagocytosis. Engineering of Fc glycans therefore enables fine-tuning of the therapeutic properties of monoclonal antibodies. The N-linked glycans of Fc are typically complex-type, forming a network of noncovalent interactions along the protein surface of the Cγ2 ...
Coonrod Leslie A - - 2012
Myotonic dystrophy type 1 (DM1) is a microsatellite expansion disorder caused by the aberrant expansion of CTG repeats in the 3'-untranslated region of the DMPK gene. When transcribed, the toxic RNA CUG repeats sequester RNA binding proteins, which leads to disease symptoms. The expanded CUG repeats can adopt a double-stranded ...
Ren Yunzhao R YR The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21231, - - 2012
Although significant effort is expended on identifying transcripts/proteins that are up-regulated in cancer, there are few reports on systematic elucidation of transcriptional mechanisms underlying such druggable cancer-specific targets. The mesothelin (MSLN) gene offers a promising subject, being expressed in a restricted pattern normally, yet highly overexpressed in almost one-third of ...
Lorestani Alexander A Department of Biology, Boston College, Chestnut Hill, MA 02467, - - 2012
The basal complex in Toxoplasma functions as the contractile ring in the cell division process. Basal complex contraction tapers the daughter cytoskeleton toward the basal end and is required for daughter segregation. We have previously shown that the protein MORN1 is essential for basal complex assembly and likely acts as ...
Shieh Peyton - - 2012
Fluorogenic probes activated by bioorthogonal chemical reactions can enable biomolecule imaging in situations where it is not possible to wash away unbound probe. One challenge for the development of such probes is the a priori identification of structures that will undergo a dramatic fluorescence enhancement by virtue of the chemical ...
Persano Luana - - 2012
Electron-beam scanning is used to realize spatially-controlled in situ synthesis of nanocrystals, as reported by Luana Persano and co-workers on page 5320. A smart combination of complementary nanofabrication approaches including nanoimprinting, electrospinning, and electron-beam decomposition of nanocrystal precursors, and subsequent nucleation of nanoparticles in a polymer matrix, allows the exploitation ...
Zhang Wendi - - 2012
Graphene oxide shows stress-induced toxicity properties in vivo under different pathophysiological conditions, as Yuliang Zhao, Guangjun Nie, Ying Fang, and co-workers report on page 5391. A dual-path chemical mechanism, involving the over-production of hydroxyl radicals and the formation of oxidizing cytochrome c intermediates, is responsible for the toxicity properties.
Tseng Kuo-Fu KF Department of Zoology, Oregon State University, Corvallis, OR 97331, - - 2012
Prior to cell cleavage, cytokinetic proteins are recruited into the nascent actomyosin contractile ring, paving the way for formation of a functional cleavage furrow. Interactions between spindle microtubules and the cell cortex may play a critical role in this recruitment, since microtubules have been shown to affect distribution and activation ...
Gudejko Heather F M HF Department of Biology, Boston College, Chestnut Hill, MA 02467, - - 2012
Vesicle trafficking and new membrane addition at the cleavage furrow have been extensively documented. However, less clear is the old idea that expansion at the cell poles occurs during cytokinesis. We find that new membrane is added to the cell poles during anaphase, causing the plasma membrane to expand coincident ...
Sanger Jean M JM Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY 13210, - - 2012
The assembly of actin into a cleavage furrow is accompanied by disassembly of the interphase actin cytoskeleton. A variation of this actin filament disassembly/assembly cycle is seen during cell division in PtK2 cells infected with the intracellular pathogen, Listeria monocytogenes, where F-actin associates with the bacteria either as a halo ...
Pokhrel Laxman L Department of Chemistry, 213 CBC Building, Kansas State University, Manhattan, Kansas 66503, - - 2012
A major effort in Alzheimer's disease therapeutic development has targeted Aβ and downstream events. We have synthesized a small library of tricyclic pyrone compounds. Their protective action in MC65 cells and inhibition of ACAT along with the upregulation of cholesterol transporter gene were investigated. Five active compounds exhibited potencies in ...
Kumar Karuppannan Senthil - - 2012
Self-assembled monolayers of DNA on a silver surface were prepared and characterized by polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS), fluorescence imaging, and (32)P radioactive labeling. The buffer concentration of the DNA solution and the surface roughness of the silver substrate were found to affect the surface coverage of DNA ...
Ameer Fathima S - - 2012
Presented herein is a combined experimental and computational study of the gold nanoparticle (AuNP) inner filter effect on surface enhanced Raman spectroscopic (SERS) measurements. Using a bianalyte strategy in which dithiopurine (DTP) and ethanol were employed as the model analytes, we demonstrated that AuNPs enhance DTP's Raman signal but attenuate ...
Jiang Tao-Shan - - 2012
A palladium-catalyzed ortho-alkoxylation of anilides with both primary and secondary alcohols via ligand-directed C-H activation has been explored. This alkoxylation promoted by catalytic methanesulfonic acid proceeds well at room temperature in most cases and affords aryl alkyl ethers in moderate to good yields.
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