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Pandey Alok K - - 2012
Polycyclic aromatic hydrocarbons (PAHs) are wide spread industrial pollutants that are released into the environment from burning of coal, distillation of wood, operation of gas works, oil refineries, vehicular emission, and combustion process. In this study a lipolytic bacterium was isolated from mixed stover compost of Saccharum munja and Brassica ...
Rivenbark Ashley G AG Department of Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, The - - 2012
An obstacle in the treatment of human diseases such as cancer is the inability to selectively and effectively target historically undruggable targets such as transcription factors. Here, we employ a novel technology using artificial transcription factors (ATFs) to epigenetically target gene expression in cancer cells. We show that site-specific DNA ...
Barrault Christine - - 2012
Sebocytes originate from the same lineage as keratinocytes, and both cell types may have similarities in terms of growth and differentiation. We were interested in studying the behaviour of human sebocytes when cultured in conditions validated for epidermal reconstruction. For this purpose, we established a HPV16-E6/7-immortalized human sebocyte cell line ...
Melanson Stacy E F - - 2012
Urine buprenorphine screening is utilized to assess buprenorphine compliance and to detect illicit use. Robust screening assays should be specific for buprenorphine without cross-reactivity with other opioids, which are frequently present in patients treated for opioid addiction and chronic pain. We evaluated the new Lin-Zhi urine buprenorphine enzyme immunoassay (EIA) ...
Zhu Jie - - 2012
Persistent changes in behavior and psychological function that occur as a consequence of exposure to drugs of abuse are thought to be mediated by the structural plasticity of specific neural circuits such as the brain's dopamine (DA) system. Changes in dendritic morphology in the nucleus accumbens (NAc) accompany drug-induced enduring ...
Motta L - - 2012
Objective: To evaluate retrospectively the efficacy of syringosubarachnoid shunt for the management of syringohydromyelia/syringomyelia. Methods: Eleven dogs diagnosed with syringohydromyelia/syringomyelia by magnetic resonance imaging associated with Chiari-like malformation underwent placement of a syringosubarachnoid shunt at the cervical (nine dogs) or lumbar (two dogs) spinal cord. In one dog, a suboccipital ...
Zhao Cunyou - - 2012
To rapidly determine DNA methylation levels from a large number of biological or clinical samples, we have developed an accurate and sensitive method for high-throughput quantification of global methylation of 5'-Cm ( 5) CGG-3' sites in the genome, visualized by fluorescence polarization (FP) based measurement of DNA methylation (FPDM). In ...
Ganten M K - - 2012
A proven criterion for assessing tumor response is the increase in tumor size. Unlike most tumors, lymph nodes are normal anatomical structures and can be measured even when benign. The International Working Group (IWG) criteria for lymphomas therefore combine morphological with functional (positron emission tomography PET) and biopsy (bone marrow ...
Stroupe Christopher C Department of Molecular Physiology and Biological Physics and Center for Membrane Biology, University of Virginia, Charlottesville, VA 22908, USA. - - 2012
A previous report described lipid mixing of reconstituted proteoliposomes made using lipid mixtures that mimic the composition of yeast vacuoles. This lipid mixing required SNARE {SNAP [soluble NSF (N-ethylmaleimide-sensitive factor)-attachment protein] receptor} proteins, Sec18p and Sec17p (yeast NSF and α-SNAP) and the HOPS (homotypic fusion and protein sorting)-Class C Vps ...
Yang Pei-Ming - - 2012
CD1d is a MHC class-like molecule that presents glycolipids to natural killer T (NKT) cells, then regulates innate and adaptive immunity. The regulation of CD1d gene expression in solid tumors is still largely unknown. Gene expression can be epigenetically regulated by DNA methylation and histone acetylation. We found that histone ...
Sinnberg Tobias - - 2012
Y-box binding protein 1 (YB-1) is an oncogenic transcription and translation factor and is overexpressed in several types of cancer. Our previous data showed that YB-1 is upregulated and translocated to the nucleus during melanoma progression and that YB-1 is an important transcription factor regulating proliferation, survival, migration, invasion and ...
Hu Kang - - 2012
An adaptive network based fuzzy inference system (ANFIS) model was employed to predict effluent chemical oxygen demand (COD(eff)) and ammonia nitrogen (NH(4) (+) (eff)) from an anaerobic/anoxic/oxic (A(2)/O) process, and meanwhile a self-adapted fuzzy c-means clustering algorithm was used to identify the model's architecture and optimize fuzzy rules. When constructing ...
Moriwaki Shinichi - - 2012
We describe an unusual xeroderma pigmentosum (XP) patient with a mutation in XP complementation group G, representing only the third reported Japanese XP-G patient. A 40-year-old men (XP3HM), born from consanguineous parents experienced sun sensitivity and pigmentary changes of sun-exposed skin since childhood. He developed a squamous cell carcinoma on ...
Shrestha Nabin K - - 2012
Mandatory infectious disease consultation for parenteral antimicrobials at hospital discharge resulted in avoiding postdischarge parenteral antimicrobials in 28% of patients. No emergency department visit or rehospitalization within 30 days for these patients was a consequence of parenteral antimicrobial avoidance. Antimicrobial stewardship at transition of care is effective in reducing unnecessary ...
Young Guang-Huar - - 2012
Epigenetic regulation plays a major role in uremic toxin-induced chronic kidney disease (CKD) progression. The KLOTHO protein is a key modulator of homeostasis in renal function. Uremic toxin accumulation can induce DNA methyltransferase (DNMT) protein expression, which is involved in the silencing of KLOTHO through hypermethylation. Treatment with DNMT inhibitors ...
Løvendorf Marianne B - - 2012
MicroRNAs are non-coding RNA molecules modulating gene expression post-transcriptionally. Formalin-fixed, paraffin-embedding (FFPE) is a standard preservation method often used in clinical practices, but induces RNA degradation. Extracting high-quality RNA from human skin can be challenging as skin contains high levels of RNases. As microRNAs are 19-23 nucleotides long and lack ...
McIntyre Iain M - - 2012
Carisoprodol is a therapeutic and occasionally abused centrally acting muscle relaxant. We compare central blood and liver concentrations of carisoprodol and the metabolite meprobamate to concentrations in peripheral blood in 11 medical examiner cases. Specimens were initially screened for alcohol and simple volatiles by gas chromatography (GC)-flame ionization detection headspace ...
Donde Aneesh A Human Exposure Laboratory, Division of Occupational and Environmental Medicine, University of California San Francisco, San Francisco, CA, - - 2012
Intermittent moderate-intensity exercise is used in human inhalational exposure studies to increase the effective dose of air pollutants. To investigate the inflammatory, coagulatory, and autonomic effects of intermittent moderate-intensity exercise. We measured hemodynamic, electrocardiographic, inflammatory, and coagulatory parameters in peripheral blood of 25 healthy subjects across an exercise protocol that ...
Tejedor Alonso M A - - 2012
Relatively few studies have examined the incidence of anaphylaxis in the general population. To report the incidence of anaphylaxis among the general population of the city of Alcorcon, Spain, using various public health care databases. Episodes of anaphylaxis were recovered using validated alphanumeric strings in different fields of electronic clinical ...
Kameda Tomohito - - 2012
Mg-Al oxide obtained by calcination of NO(3) (-)-intercalated Mg-Al layered double hydroxide (NO(3)•Mg-Al LDH) was used to treat H(2)SO(4), acting as both a neutralizer of the acid and a fixative for SO(4) (2-). The fraction of SO(4) (2-) removed increased with time and with increasing Mg-Al oxide quantity and temperature. ...
Levy E R - - 2012
Objective and design. Antimicrobial use in hospitalized children has not been well described. To identify targets for antimicrobial stewardship interventions, we retrospectively examined pediatric utilization rates for 48 antimicrobials from 2007 to 2010 as well as appropriateness of vancomycin and cefepime use in 2010. Patients and setting. All children hospitalized between 2007 ...
Stammers A T - - 2012
Many therapies that have been developed for acute spinal cord injury (SCI) either influence or are influenced by posttraumatic inflammation. Many such therapies have reportedly produced promising neurologic benefits in animal models of SCI, but demonstrating convincing efficacy in human clinical trials has remained elusive. This discrepancy may be related ...
Saalbach Anja - - 2012
Psoriasis is an inflammatory skin disease often associated with obesity. The anti-inflammatory adipokine vaspin, a suggested serine proteinase inhibitor of the serpin family, is discussed as a new link between inflammation and obesity. Here, we demonstrate that - different from healthy controls - vaspin serum levels in patients with psoriasis ...
Doupé David P DP Department of Physiology, Development and Neuroscience, The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, - - 2012
In the 1970s, studies of tissue architecture and cell proliferation were used to formulate a new model of epidermal homeostasis. This asserted that the tissue was maintained by long-lived, slow-cycling, self-renewing stem cells that generate a short-lived population of transit amplifying (TA) cells, which undergo terminal differentiation after a set ...
Khanitchaidecha Wilawan - - 2012
Higher concentrations of ammonium (NH(4)-N) and iron (Fe) than a standard for drinking are typical characteristics of groundwater in the study area. To remove NH(4)-N and Fe, the drinking water supply system in this study consists of a series of treatment units (i.e., aeration and sedimentation, filtration, and chlorination); however, ...
Schroeder James W - - 2012
Prenatal exposure both to maternal psychiatric illness and psychiatric medication has been linked with adverse child outcomes that affect physiological, emotional and psychiatric development. Studies suggest that epigenetic mechanisms, such as DNA methylation, may facilitate these effects. In this report, we explore the association between maternal psychiatric illness and treatment ...
Shanks Kevin G - - 2012
Synthetic cannabinoids have been detected in various herbal blends sold legally in convenience stores, smoke shops, and on the Internet. Many of these compounds have extreme forensic significance. We developed and validated a rapid ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of trace concentrations of two of these ...
Collings A J - - 2012
Objectives: Hypoglycaemia is a common cause of morbidity in dogs with congenital portosystemic shunts but the aetiology is unknown. The hypothesis of this study was that dogs with congenital portosystemic shunts would have significantly higher insulin concentrations than dogs without congenital portosystemic shunts. The main objective of the study was ...
Vetander M - - 2012
Information about acute reactions to foods among children is limited. To describe the overall incidence of anaphylaxis in a paediatric emergency department (ED) setting and to describe reactions to foods in relation to sex and age, clinical characteristics and management. In a review of medical records, children with ED visits ...
Xie Kun - - 2012
Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) are a group of important persistent organic pollutants. In the present study, the three-dimensional holographic vector of atomic interaction field (3D-HoVAIF) method is used to describe the chemical structures of PCDD/Fs. After variable screening using a stepwise multiple regression (SMR) technique, the linear relationships among ...
Almeida Rimple D RD Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), School of Clinical Sciences, University of Nottingham, Nottingham, - - 2012
5-hydroxymethyl-cytosine (5-hmC) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (ESC) derived from mammalian blastocysts. Recent observations imply that both 5-hmC and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmC, may play an important role in self renewal and differentiation of ...
Vickers Kasey C - - 2012
Extracellular microRNAs (miRNAs) are uniquely stable in plasma, and the levels of specific circulating miRNAs can differ with disease. Extracellular miRNAs are associated with lipid-based carriers and lipid-free proteins. miRNAs can be transferred from cell-to-cell by lipid-based carriers and affect gene expression. This review summarizes recent studies that demonstrate the ...
Kook Janet L - - 2012
A retrospective, quasi-experimental cohort study compared antibiotic use before and after implementation of a procalcitonin assay at a community acute care hospital. This study demonstrated that the implementation of the procalcitonin assay was associated with a decrease in antibiotic days of therapy in adult patients with pneumonia.
Menne Jan - - 2012
We have previously demonstrated in the Randomized Olmesartan and Diabetes Microalbuminuria Prevention study that the angiotensin receptor blocker (ARB) olmesartan delays the onset of microalbuminuria in patients with type 2 diabetes. Now, we investigated the effect in the subpopulation with hypertension. Overall, 4020 patients with type 2 diabetes and hypertension ...
Saeki Yuko - - 2012
A calcium gradient in skin epidermis is known to regulate epidermal differentiation. In cultures of human epidermal keratinocytes (NHEK), induction of calcium-dependent differentiation is associated with phosphorylation of ErbB receptors, including the epidermal growth factor receptor (EGFR). The activation of EGFR triggers the induction of activator protein 1 (AP-1) proteins ...
Beardsley James R - - 2012
The financial impact of an antimicrobial stewardship program in operation for more than 11 years was determined by calculating the reduction in antimicrobial expenditures minus program labor costs. Depending on the method of inflation adjustment used, the program was associated with average cost savings of $920,070 to $2,064,441 per year.
Han Gee-Bong - - 2012
In this study, the feasibility of an innovative upflow packed-bed reactor (UPBR) system using porous sulfur and lime ceramic media (CERAMED-L and CERAMED-SL) to remove nitrogen for wastewater treatment was evaluated. The specific lime dissolution rates for CERAMEDs in the UPBRs show an inverse proportion to pH and resulted in ...
Pahr S - - 2012
Wheat (Triticum aestivum) is an important allergen source responsible for various clinical manifestations of allergy (i.e. food allergy, pollen allergy, respiratory allergy to flour-Baker's asthma). The objective of this study was the molecular and immunological characterization of new recombinant wheat allergens and to evaluate their usefulness for the diagnosis of ...
Meisgen Florian - - 2012
MicroRNAs are short non-coding RNAs that regulate gene expression. Previously, in a genome-wide screen, we found deregulation of microRNA expression in psoriasis skin. MicroRNA-21 (miR-21) is one of the microRNAs significantly up-regulated in psoriasis skin lesions. To identify the cell type responsible for the increased miR-21 level, we compared expression ...
Hansmann Britta - - 2012
The S100 fused-type proteins (SFTPs) are thought to be involved in the barrier formation and function of the skin. Mutations in the profilaggrin gene, one of the best investigated members of this family, are known to be the major risk factors for ichthyosis vulgaris and atopic dermatitis. Recently, we identified ...
- - 2012
A short-cut review was carried out to establish whether Bell's palsy is best treated with a combination of prednisolone and an antiviral agent or prednisolone alone. Four systematic reviews and two small studies were directly relevant to the question. The author, date and country of publication, patient group studied, study ...
Cosgrove Sara E - - 2012
Objective. To evaluate the impact of postprescription review of broad-spectrum antimicrobial (study-ABX) agents on rates of antimicrobial use. Design. Quasi-experimental before-after study. Setting. Five academic medical centers. Patients. Adults receiving at least 48 hours of study-ABX. Methods. The baseline, intervention, and follow-up periods were 6 months each in 2 units at each of 5 sites. ...
Elligsen Marion - - 2012
Objective. We aimed to rigorously evaluate the impact of prospective audit and feedback on broad-spectrum antimicrobial use among critical care patients. Design. Prospective, controlled interrupted time series. Setting Single tertiary care center with 3 intensive care units. Patients and interventions. A formal review of all critical care patients on their third or tenth day ...
Mahoney Sarah E - - 2012
Rhabdomyosarcoma is the most common soft-tissue sarcoma in children. While cytogenetic abnormalities have been well characterized in this disease, aberrant epigenetic events such as DNA hypermethylation have not been described in genome-wide studies. We have analyzed the methylation status of 25,500 promoters in normal skeletal muscle, and in cell lines ...
Esquivel-Rodríguez Juan J Department of Computer Science, Purdue University, West Lafayette, Indiana 47907, - - 2012
A novel computational method for fitting high-resolution structures of multiple proteins into a cryoelectron microscopy map is presented. The method named EMLZerD generates a pool of candidate multiple protein docking conformations of component proteins, which are later compared with a provided electron microscopy (EM) density map to select the ones ...
Kunisue Tatsuya - - 2012
Benzophenone (BP)-type UV filters are widely used in a variety of personal care products for the protection of skin and hair from UV irradiation. Despite the estrogenic potencies of BP derivatives, few studies have examined the occurrence of these compounds in human matrices. Furthermore, associations among exposure to these compounds ...
Boyer Nicholas P NP Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, - - 2012
Lipofuscin is a fluorescent material with significant phototoxic potential that accumulates with age in the retinal pigment epithelium (RPE) of the eye. It is thought to be a factor in retinal degeneration diseases. The most extensively characterized lipofuscin component, N-retinylidene-N-retinylethanolamine (A2E), has been proposed to be a byproduct of reactions ...
Wu Pan - - 2012
4-Oxalocrotonate tautomerase (4-OT), a member of tautomerase superfamily, is an essential enzyme in the degradative metabolism pathway occurring in the Krebs cycle. The proton transfer process catalyzed by 4-OT has been explored previously using both experimental and theoretical methods; however, the elaborate catalytic mechanism of 4-OT still remains unsettled. By ...
Masutomi Kosuke K Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, - - 2012
All-trans retinal is a potent photosensitizer that is released in photoreceptor outer segments by the photoactivated visual pigment following the detection of light. Photoreceptor outer segments also contain high concentrations of polyunsaturated fatty acids, and are thus particularly susceptible to oxidative damage such as that initiated by light via a ...
Burgos Emmanuel S ES Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, United - - 2012
S-Adenosyl-L-homocysteine hydrolase (SAHH) catalyzes the reversible conversion of S-adenosyl-L-homocysteine (SAH) to adenosine (ADO) and L-homocysteine, promoting methyltransferase activity by relief of SAH inhibition. SAH catabolism is linked to S-adenosylmethionine metabolism, and the development of SAHH inhibitors is of interest for new therapeutics with anticancer or cholesterol-lowering effects. We have developed ...
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