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Paulson M L - - 2012
Congenital contractural arachnodactyly (CCA) is caused by mutations within the fibrillin-2 gene (FBN2), which is crucial for microfibril structure. Affected individuals may have contractures, chest wall deformities, scoliosis, abnormal ear folding and elongated limbs. We describe a novel FBN2 mutation in a woman with CCA who also had pulmonary non-tuberculous ...
Parks Tom T Wellcome Trust Centre for Human Genetics, Oxford, - - 2012
In resource-limited tropical settings, both impetigo and rheumatic disease are endemic. The major cause of impetigo in these regions is the group A streptococcus and there is a growing body of opinion implicating impetigo in the pathogenesis of rheumatic fever and rheumatic heart disease (RHD). This potentially has major implications ...
Vaidya Anand A Division of Endocrinology, Diabetes, and Hypertension, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, - - 2012
Over the past decade, vitamin D has generated considerable interest as potentially having important effects on the vasculature and the kidney. Animal and human data indicate that vitamin D suppresses the activity of the renin-angiotensin system and improves endothelial function. Observational studies in humans suggest that low 25-hydroxyvitamin D (25[OH]D) ...
Smith Rachel A RA Department of Communication Arts & Sciences, Center for Infectious Disease Dynamics, & Methodology Center, The Pennsylvania State University, 318 Sparks Bldg, University Park, PA 16802, USA. - - 2012
Social network analysis was used to examine the relationship between HIV/AIDS stigmatization, perceived risk, and centrality in the community network (via participation in community groups). The findings from respondents in Keetmanshoop, Namibia (N = 375) showed an interaction between stigma and risk perceptions\hose who perceived higher HIV risk and stronger ...
Anker Justin J JJ Department of Psychiatry, University of Minnesota Medical School, Minneapolis, MN 55455, USA. - - 2012
Progesterone decreases cocaine self-administration in women and in female rats. In a previous study using rats selectively bred for high (HiS) or low (LoS) saccharin intake, HiS rats escalated their cocaine intake compared with LoS rats. Our goal was to examine the effects of progesterone on the escalation of cocaine ...
Hardy Olga T OT Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, - - 2012
The association between obesity and insulin resistance is an area of much interest and enormous public health impact, with hundreds of articles being published in the last year focused on the possible mechanisms that underlie this association. The purpose to this review is to highlight some of the key recent ...
Byrne Guerard W GW University College London, Institute for Cardiovascular Sciences, London, UK. - - 2012
Cardiac xenotransplantation (CXTx) remains a promising approach to alleviate the chronic shortage of donor hearts. This review summarizes recent results of heterotopic and orthotopic CXTx, highlights the role of non-Gal antibody in xenograft rejection, and discusses challenges to clinical orthotopic CXTx. Pigs mutated in the α 1,3 galactosyltransferase gene (GTKO ...
Milić Marija M Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, - - 2012
Benzodiazepines negatively affect motor coordination and balance and produce myorelaxation. The aim of the present study was to examine the extent to which populations of γ-aminobutyric acid A (GABAA) receptors containing α1 and α5 subunits contribute to these motor-impairing effects in rats. We used the nonselective agonist diazepam and the ...
Bastani Nasser E - - 2012
F2-isoprostanes are a series of prostaglandin F2-like compounds that are formed by free-radical-catalyzed peroxidation of arachidonic acid (ARA). Several F2-isoprostanes, but in particular 8-epi-PGF(2α) , are widely used as oxidative stress biomarkers. In this study we have developed an analytical tool for finger-tip blood sampling and analysis of 8-epi-PGF(2α) from ...
Liao Shengkai - - 2012
It has been proposed that the increase in the instances of idiosyncratic adverse drug reactions (IADRs) and black box warnings may be attributed to the occurrence of reactive metabolites. Consequently, a high-throughput screen for reactive metabolites formed from liver microsome extracts with added glutathione (GSH) was developed for use in ...
Deng Kai - - 2012
The nanostructure-initiator mass spectrometry based enzyme assay (Nimzyme) provides a rapid method for screening glycan modifying reactions. However, this approach cannot resolve stereospecific reactions which are common in glycobiology and are typically assayed using lower-throughput methods (gas chromatography/mass spectrometry (GC/MS) or liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis) often in conjunction ...
Couderc Carine - - 2012
It is recommended that harmful Biosafety Level 3 (BSL-3) bacteria be inactivated prior to identification by mass spectrometry, yet optimal effects of inactivation protocol have not been defined. Here, we compare trifluoroacetic acid inactivation (protocol A) with ethanol inactivation (protocol B) of Yersinia organisms prior to identification by matrix-assisted laser ...
Bass Adrian M - - 2012
Quantifying the processes that control dissolved inorganic carbon (DIC) dynamics in aquatic systems is essential for progress in ecosystem carbon budgeting. The development of a methodology that allows high-resolution temporal data collection over prolonged periods is essential and is described in this study. A novel sampling instrument that sequentially acidifies ...
Steppert M - - 2012
Polymerization in hexavalent uranium solutions was measured by electrospray ionization time-of-flight mass spectrometry in three different acidic media at pH values from 3 to 5.3 in order to detect all hydrolysis species present in solution. The aqueous solutions were directly measured without further dilution in organic solvents. At high uranyl ...
Muntel Jan - - 2012
Label-based mass spectrometry is a powerful tool for large-scale protein identification and quantification. However, it requires the chemical or metabolic incorporation of the labeled compound(s) which can be difficult to attain, e.g. for non-cultivable organisms or scarce sample, such as biopsies. Therefore, we set out to develop and validate an ...
Fritzsche Romy - - 2012
Chemical cross-linking in combination with a mass spectrometric analysis of the created cross-linked products is an area of growing interest for deriving low-resolution structural information of proteins and protein complexes. One of the greatest challenges is the complexity of the created cross-linking mixtures, which can be met by a charge-based ...
Esposito Simone - - 2012
Mechano growth factor (MGF) is a splice variant of insulin-like growth factor that possesses anabolic properties and has not yet been approved for therapeutic use. Nevertheless, it is readily available on the black market. Although the World Anti-Doping Agency (WADA) has banned the use of MGF in sports, no routinely ...
Leis Hans J - - 2012
The synthesis of a novel chiral derivatisation reagent, (S)-(-)-N-(pentafluorobenzylcarbamoyl)prolyl chloride is described which is preferably useful for negative-ion chemical ionisation mass spectrometry. Preparation of the reagent followed a general strategy used to prepare enantioselective reagents based on the N-substitution of L-proline. Pentafluorobenzyl chloroformate smoothly reacted with L-proline to give the ...
Sekiya Sadanori - - 2012
3-Aminoquinoline/α-cyano-4-hydroxycinnamic acid (3AQ/CHCA) is a liquid matrix (LM), which was reported by Kumar et al. in 1996 for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It is a viscous liquid and has some advantages of durability of ion generation by a self-healing surface and quantitative performance. In this study, we found ...
Wang Hao-Yang - - 2012
Hypervalent iodine compounds are important and widely used oxidants in organic chemistry. In 2005, Ochiai reported the PhI-catalyzed α-acetoxylation reaction of acetophenone by the oxidation of PhI with m-chloroperbenzoic acid (m-CPBA) in acetic acid. However, until now, the most critical reactive α-λ(3) -iodine alkyl acetophenone intermediate (3) had not been ...
Crespo E - - 2012
Several mycobacterial species can produce serious infections in humans, and the treatment required depends on the infecting species. Fast identification, ideally with minimal manipulation of the infecting species, is therefore critical; here, we propose a method potentially allowing cultures to be identified by headspace analysis and use it to screen ...
Koulakiotis Nikolaos Stavros - - 2012
The expensive spice saffron originating from the stigmas of Crocus sativus L. and also applied in traditional Chinese medicine (TCM) constitutes a complex mixture of glycoconjugates varying not only in the aglycon structure, but also in glycosylation pattern. Therefore, various tandem mass spectrometric techniques were evaluated for their usefulness in ...
Yang Hyo-Jik - - 2012
A combination of methodologies using the extremely high mass accuracy and resolution of 15-T Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) was introduced for the identification of intact cancer cell phospholipids. Lipids from a malignant glioma cell line were initially analyzed at a resolution of >200 000 and ...
Simon E S - - 2012
Motivated by the need for chemical strategies designed to tune peptide fragmentation to selective cleavage reactions, benzyl ring substituent influence on the relative formation of carbocation elimination (CCE) products from peptides with benzylamine-derivatized lysyl residues has been examined using collision-induced dissociation (CID) tandem mass spectrometry. Unsubstituted benzylamine-derivatized peptides yield a ...
Marteau Charlotte - - 2012
Polybrominated diphenyl ethers (PBDEs) are flame retardants widely used in electronic and domestic goods. These persistent pollutants are present in the environment and in humans, and their toxicological properties are of growing concern. PBDEs can be metabolised into compounds suspected to be responsible for their toxicity. These metabolites have been ...
Ryu Yun-Kyoung - - 2012
Thymosin beta-4 (Tβ4), actin-sequestering protein, plays important roles in many cellular functions including cancer cell migrations. Glycogen synthase kinase (GSK) in Wnt signaling pathway is a key molecule to control intercellular interaction. Here, we investigated whether GSK-3 activity is regulated by Tβ4 and it is associated with Tβ4-mediated migration in ...
Martín-Carbonero Luz - - 2012
Single nucleotide polymorphisms (SNP) nearby the IL28B gene have been associated with spontaneous hepatitis C virus (HCV) clearance and response to interferon-based therapies in both monoinfected and HIV-coinfected patients. However, its impact on spontaneous clearance of HBV (the pathogenesis of which is also related to interferon) is less known. A ...
Davies John R - - 2012
Inherited MC1R variants modulate MITF transcription factor signaling, which in turn affects tumor cell proliferation, apoptosis, and DNA repair. The aim of this BioGenoMEL collaborative study in 10 melanoma cohorts was to test the hypothesis that inherited variants thereby moderate survival expectation. A survival analysis in the largest cohort (Leeds) ...
Kraus James M - - 2012
Inhibitors of neuronal nitric oxide synthase, based on a chiral pyrrolidine scaffold, show promise for the treatment of certain neurodegenerative diseases. We recently reported the synthesis of a series of selective inhibitors, but the method was limited at a key step of formingan allyl ether intermediate. Yields for this step ...
Serrot Patricia H - - 2012
Three evergreen (Laurus nobilis, Viburnum tinus and Thuja plicata) and two autumnal abscission deciduous trees (Cydonia oblonga and Prunus domestica) have been investigated for the presence (zymogram and immunodetection) and functionality (post-illumination chlorophyll fluorescence) of the thylakoid Ndh complex. The presence of encoding ndh genes has also been investigated in ...
Locatelli Denise D Molecular Neuroanatomy and Pathogenesis Unit, Neurological Institute 'C. Besta', Milano, - - 2012
The axonal survival of motor neuron (a-SMN) protein is a truncated isoform of SMN1, the spinal muscular atrophy (SMA) disease gene. a-SMN is selectively localized in axons and endowed with remarkable axonogenic properties. At present, the role of a-SMN in SMA is unknown. As a first step to verify a ...
Li Fu - - 2012
The limited therapeutic effect of gemcitabine on pancreatic cancer is largely attributed to pre-existing or acquired resistance of the tumor cells. This study was aimed at screening for candidate resistance-related gene(s) and elucidating the underlying mechanisms. NME5 was found to be highly expressed in an innate gemcitabine-resistant human pancreatic cancer ...
Mattingly Carolyn J - - 2012
Environmental health information resources lack exposure data required to translate molecular insights, elucidate environmental contributions to diseases, and assess human health and ecological risks. We report development of an Exposure Ontology, ExO, designed to address this information gap by facilitating centralization and integration of exposure data. Major concepts were defined ...
Kun Alejandra A Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, - - 2012
Very little is known about the function of the F-actin cytoskeleton in the regeneration and pathology of peripheral nerve fibers. The actin cytoskeleton has been associated with maintenance of tissue structure, transmission of traction and contraction forces, and an involvement in cell motility. Therefore, the state of the actin cytoskeleton ...
Shen Yao-Qing YQ Department of Chemistry, University of California, Berkeley, California 94720, United - - 2012
Pyrroloquinoline quinone (PQQ) is a small, redox active molecule that serves as a cofactor for several bacterial dehydrogenases, introducing pathways for carbon utilization that confer a growth advantage. Early studies had implicated a ribosomally translated peptide as the substrate for PQQ production. This study presents a sequence- and structure-based analysis ...
Dzikovski Boris B National Biomedical Center for Advanced ESR Technology, Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, New York 14853, - - 2012
The ESR parameters of PC spin labels in frozen membranes do not simply represent the membrane polarity or water penetration profile. Instead, they show a distribution between hydrogen-bonded (HB) and non-hydrogen-bonded (non-HB) states, which is affected by a number of factors in the membrane composition. Similar to the exclusion of ...
Hegan Peter S PS Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, - - 2012
Characterization of the intestinal epithelium of the Snell's waltzer (sv/sv) mouse revealed that myosin VI (Myo6) is required for proper brush border (BB) ultrastructure, composition and membrane traffic. The defects observed were distinct from that observed in the myosin Ia KO, even though Myo6 is lost from the BB in ...
Shenkin Jonathan D - - 2012
PURPOSE/QUESTION: To determine the outcomes of completing a pulpectomy on primary teeth that have been determined to have a necrotic pulp. SOURCE OF FUNDING: Information not available. TYPE OF STUDY/DESIGN: Case series. LEVEL OF EVIDENCE: Level 3: Other evidence. STRENGTH OF RECOMMENDATION GRADE: N/A: Not applicable.
Larson A Noelle - - 2012
: Previous long-term studies have shown good outcomes for most patients after in situ pinning of slipped capital femoral epiphyses (SCFE). However, concern is growing about the effects of leaving the epiphysis in a nonanatomic position. We undertook a retrospective study to carefully document patient-reported outcomes and need for additional ...
Glatman-Freedman Aharona - - 2012
Vaccine preventable diseases have been responsible for a significant portion of childhood mortality in low-income countries, and have been re-emerging in medium- and high-income countries. The effectiveness of routine childhood immunization programs relies on multiple factors. Social determinants have the potential to affect immunization programs around the world, with globalization ...
Lee Kyoung Min - - 2012
: The choice of pinning techniques in supracondylar fractures of the humerus in children has been a debate regarding its fixation stability and risk of iatrogenic ulnar nerve palsy. This study was performed to determine as to which fixating method (medial and lateral crossed pinning vs. lateral pinning) is better ...
Chapman Rose - - 2012
Aim.  The aim of the study was to assess health professionals' knowledge, attitudes and beliefs regarding lesbian, gay, bisexual and transgender parents accessing health care for their children. Background.  Lesbian, gay, bisexual and transgender parents can be reluctant to reveal their sexual orientation to health professionals, and consequently, they may ...
Esparza Jose - - 2012
This book (History of Vaccine Development, edited by Stanley Plotkin, Springer, 2011, 349 pages) collects the personal perspectives of selected scientists who were instrumental in the development of a number of current vaccines. The editor of this book, Stanley Plotkin, is an emeritus professor of the University of Pennsylvania and ...
Verardi Simone - - 2012
PURPOSE/QUESTION: Does apical surgery affect periodontal parameters? SOURCE OF FUNDING: Information not available. TYPE OF STUDY/DESIGN: Case series. LEVEL OF EVIDENCE: Level 3: Other evidence. STRENGTH OF RECOMMENDATION GRADE: Not applicable.
Jesuthasan Suresh - - 2012
Emotional responses are triggered by environmental signals and involve profound changes at multiple levels, from molecular to behavior. Much has been learnt about two emotions, fear and anxiety, by studying mammalian models. In particular, neural circuits and the corresponding molecular mechanisms essential for the learning and retention of fear, as ...
Fraser Kristin - - 2012
Medical Education 2012: 46: 299-305 Context  Retroactive interference occurs when newly acquired information inhibits recall of previously learned information. This has been shown to influence recall of sounds, tastes and word associations, and is typically seen when learners receive training on one area of content and are then exposed to ...
Azarpazhooh Amir - - 2012
PURPOSE/QUESTION: To determine the influence of root canal treatment on pain prevalence and severity and estimate the prevalence and severity of pretreatment, treatment, and posttreatment pain in patients receiving nonsurgical root canal treatment. SOURCE OF FUNDING: The University of California, Los Angeles, Section of Endodontics. TYPE OF STUDY/DESIGN: Systematic review ...
Gudjonsson Johann E - - 2012
DNA methylation is one of several epigenetic mechanisms that can lead to stable and heritable changes in gene expression without changing the underlying DNA sequence. In this issue, Roberson et al. demonstrate that lesional skin in psoriasis is accompanied by changes in DNA methylation, which revert back to baseline with ...
Awasthi Shally - - 2012
Background: Asthma is a complex genetic disorder. Several genes have been found associated with asthma. The cystic fibrosis transmembrane conductance regulator (CFTR) gene is one of them. Aim: To assess the association of CFTR gene mutation with asthma and its severity as per GINA guidelines. Subjects and methods: This was ...
Brown James H - - 2012
[Formula: see text] [ Many landscapes span communities of varying degrees of biological complexity. Across such a gradient, diversity begets diversity. Increased environmental heterogeneity in the Mojave Desert increases the diversity of rodent consumers, in particular by allowing species that are phylogenetically more similar to coexist in more species rich ...
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