Search Results
Results 1 - 50 of 1016
1 2 3 4 5 6 7 8 9 10 >
Stern Michael - - 2012
Confocal image of a locust brain 14 days after one antenna had been crushed. Antennal afferents were anterogradely labeled with Neurobiotin (magenta). Aberrantly regenerated olfactory receptor axons have grown through the antennocerebral tract into the mushroom body. Counterstaining: phalloidin (green) and DAPI (blue). The Journal of Comparative Neurology, Volume 520, ...
Rožman Marko - - 2012
The dissociation of protein ions (5-30 kDa) as a function of charge state has been explored in order to suggest the optimal charge state range for top-down sequencing. Proteins were generated under denaturing conditions and their charge states were modified via ion/ion proton transfer reactions prior to dissociation. Electron transfer dissociation ...
Xin Ling - - 2012
Site-specific glycoprofiling of N-glycoproteins is based on the enrichment of glycopeptides. In this study, we develop a simple and economical ZipTip microcolumn packed with a 1:1 ratio of graphite carbon to activated charcoal (w/w) (termed as GA-ZipTip microcolumn) to efficiently isolate and enrich N-glycopeptides from digested mixtures of individual glycoproteins ...
Vogl Jochen - - 2012
Triple IDMS has been applied for the first time to the quantification of element concentrations. It has been compared with single and double IDMS obtained on the same sample set in order to evaluate the advantages and disadvantages of triple IDMS over single and double IDMS as an analytical reference ...
Oliveira Sheila Rodrigues - - 2012
Sodium adducts of six organosulfur-α,ω-ditetrazole compounds (Tz-(CH(2) )(n) -S(O)(m) -(CH(2) )(n) -Tz; where Tz = tetrazole ring; n = 2, 3; m = 0, 1, 2) were generated via electrospray ionization (ESI) and their fragmentation pattern assessed via collision-induced dissociation (CID). Two main dissociation channels were observed: (a) losses of N(2) and HN(3) from the tetrazole ...
Cristoni Simone - - 2012
8-Hydroxy-2'-deoxyguanosine has served as a biomarker for oxidative damage to DNA from different types of biological samples, and various techniques have been used to analyze it. In particular, liquid chromatography coupled to mass spectrometry has been used to identify 8-hydroxy-2'-deoxyguanosine in urine samples. Usually, a triple quadrupole analyzer and multiple ...
Hui Joseph P M - - 2012
A strategy to identify metabolites of a marine biotoxin, 13-desmethyl spirolide C, has been developed using liquid chromatography coupled to high-resolution mass spectrometry (LC/HRMS). Metabolites were generated in vitro through incubation with human liver microsomes. A list of metabolites was established by selecting precursor ions of a common fragment ion ...
Santos Hugo M - - 2012
There is a need in imaging mass spectrometry to use the acquired isotope distribution to unequivocally determine the identity of a peptide ion. A way of achieving unambiguous differentiation of ions from protonated peptides from other [M + H](+) ions in a tissue would be via the direct on-tissue incorporation of (18) ...
Hunter Gerald O - - 2012
The gas-phase ligand exchange reactions between Co(II) and Zn(II) complexes containing the acetylacetonate (acac), hexafluoroacetylacetonate (hfac), and trifluorotrimethylacetylacetonate (tftm) ligands were investigated using a triple quadrupole mass spectrometer. The gas-phase mixed ligand products of [Cu(acac)(tftm)](+) , [Ni(acac)(tftm)](+) , [Cu(hfac)(tftm)](+) , and [Ni(hfac)(tftm)](+) were formed following the co-sublimation of either homo-metal ...
Amadei Gianluca - - 2012
Basil (Ocimum basilicum) is an important flavourant plant which constitutes the major ingredient of the pasta sauce 'Pesto alla Genovese'. The characteristic smell of basil stems mainly from a handful of terpenoids (methyl cinnamate, eucalyptol, linalool and estragole), the concentration of which varies according to basil cultivars. The simple and ...
Liu Jingzhou - - 2012
Metabolite identification is an important part of the drug discovery and development process. High sensitivity is necessary to identify metabolic products in vitro and in vivo. The most common method utilizes standard high-performance liquid chromatography (4.6 mm i.d. column and 1 mL/min flow rate) coupled to tandem mass spectrometry (HPLC/MS/MS). We have ...
Nielsen Katrine - - 2012
Screening of drugs from urine samples can be non-selective or laborous, using either immunological, gas chromatography/mass spectrometry (GC/MS) or liquid chromatography (LC)/MS methods. Therefore, a rapid screening method for selected drugs in urine sample was developed in a proof-of-principle manner, utilizing simple and fast techniques for both sample treatment and ...
Hubert-Roux Marie - - 2012
Tiagabine hydrochloride monohydrate drug substance (TGB) is an antiepileptic agent effective in the treatment of seizure disorders. The stability of TGB was studied and its degradation products were identified for the first time. TGB was heated in the presence of H(2) O(2) . Degradation products were analyzed by liquid chromatography ...
Chenery Carolyn A - - 2012
Oxygen isotope analysis of archaeological human dental enamel is widely used as a proxy for the drinking water composition (δ(18) O(DW) ) of the individual and thus can be used as an indicator of their childhood place of origin. In this paper we demonstrate the robustness of structural carbonate oxygen ...
Lalli Priscila M - - 2012
The ability of travelling wave ion mobility mass spectrometry (TWIM-MS) to resolve cationic meta/para and cis/trans isomers of mono-, di-, tri- and tetra-ruthenated supramolecular porphyrins was investigated. All meta isomers were found to be more compact than the para isomers and therefore mixtures of all isomeric pairs could be properly ...
Downard Kevin M - - 2012
The effect of hydroxyl radical induced oxidation on the collision cross-sections of hen egg lysozyme and bovine ubiquitin was investigated by travelling wave ion mobility mass spectrometry for the first time. The oxidized ions of lysozyme and ubiquitin share common collision cross-sections with their unoxidized counterparts suggesting that they share ...
Ingelido Anna Maria - - 2012
Due to safety concerns regarding dietary exposure to POPs, regulatory bodies are issuing detailed guidelines for testing for polychlorodibenzodioxins (PCDDs) and polychlorodibenzofurans (PCDFs) ('dioxins') and dioxin-like (DL)-PCBs in foods of animal origin. Determination of the aforesaid chemicals at regulatory levels requires highly selective and sensitive testing techniques. The new generation ...
Flamini Riccardo - - 2012
Anthocyanins confer the color to red wines. During wine aging, new anthocyanin derivatives with peculiar chromatic characteristics are formed. A fast and selective method for screening the anthocyanin composition of wine has been developed. Direct-infusion electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of wine extracts has been performed by operating ...
Schumaker Stephanie - - 2012
The use of matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF MS) has emerged as a promising tool to rapidly profile bacteria at the genus and species level and, more recently, at the sub-species (strain) level. Recently, it has been proposed that the approach can be enhanced with regard to ...
Chen Xiangfeng - - 2012
The mass spectrometric studies of transition metal ion-ether reactions are informative in elucidating the principles of metal ion mediated demethanation and dehydrogenation. In order to obtain a deeper understanding of the role of metal ions, it is essential to explore the detailed reaction mechanisms of these reactions, here studied using ...
Kuki Akos - - 2012
Dissociation of doubly cationized polyethers, namely [P + 2X](2+) into [P + X](+) and X(+) , where P = polyethylene glycol (PEG), polypropylene glycol (PPG) and polytetrahydrofuran (PTHF) and X = Na, K and Cs, was studied by means of energy-dependent collision-induced dissociation tandem mass spectrometry. It was observed that the collision voltage necessary to obtain 50% fragmentation ...
Kulkarni Sourabh U - - 2012
Quantitative analysis of partially soluble and insoluble polydisperse materials is challenging due to the lack of both appropriate standards and reliable analytical techniques. To this end, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) incorporating a solvent-free sample preparation technique was investigated for the quantitative analysis of partially soluble, polydisperse, polycyclic aromatic ...
Fiorentino Girolamo - - 2012
The discovery of a storeroom full of barley and other cereals (L.9512) in the proto-historic site of Ebla has provided a unique opportunity to study the centralized storage system of the early city-state from a different perspective. Epigraphic evidence available within the site reveals a complex system of taxation which ...
Bernhard Linden H - - 2012
To exploit the softness of liquid injection field desorption/ionization (LIFDI), the molecular ions, M(+•) , need to be transferred from their origin at the field emitter through the mass analyzer without disrupting their integrity. To preserve the molecular ions, ion-activating events like collisions must therefore be avoided. In hybrid quadrupole ...
Saang'onyo Daudi S - - 2012
Presented here are findings for the development and optimization of a simple, high-throughput, and rapid method for the analysis of glucose. Because the applications of glucose and other six-carbon sugars is a growing field of interest especially in the production of biofuels, an efficient and rapid method for their quantitation ...
Mbah Nsehniitooh A - - 2012
  In laparoscopic liver resection, multiple options for parenchymal transection techniques exist; however, none have emerged as superior. The aim of this study was to compare operative characteristics and outcomes between bipolar compression and ultrasonic devices used for parenchymal transection during laparoscopic liver resection.   A review of a prospective ...
Augoustides John G T - - 2012
Recent landmark randomized trials and society guidelines have significantly revised the management of carotid artery disease. Duplex ultrasonography is the recommended initial diagnostic test for the assessment of extracranial carotid artery stenosis. Carotid artery imaging is reasonable in select patients scheduled for coronary artery bypass graft (CABG) surgery. Carotid revascularization ...
Fakheri Hafez - - 2012
Sequential regimens have been recently reported to be superior to the standard triple therapies in Helicobacter pylori eradication, but most of these studies were performed in Europe and data from developing countries are lacking. So we designed a study to compare a sequential regimen with a bismuth-based quadruple therapy that ...
Yindom L-M LM Medical Research Council Laboratories, Fajara, Banjul, The Gambia. - - 2012
The relevance of innate immune responses to Plasmodium falciparum infection, in particular the central role of natural killer (NK) cell-derived interferon gamma (IFN-γ), is becoming increasingly recognised. Recently, it has been shown that IFN-γ production in response to P. falciparum antigens is in part regulated by killer-cell immunoglobulin-like receptor (KIR) ...
Li Zhuo-Ri - - 2012
Liver failure is the end stage of hepatopathy with unfavorable prognosis. In two patients with liver failure, viable primary human hepatocytes, obtained from resected liver tissue of patients with hepatolithiasis, were transplanted into the spleen by interventional therapy through femoral arterial cannula. After transplantation, the patients' clinical symptoms and liver ...
Patel Prakash A - - 2012
There have been rapid advances in oral anticoagulation. The oral factor Xa inhibitors rivaroxaban and apixaban and the oral direct thrombin inhibitor dabigatran recently have been rigorously evaluated. These novel anticoagulants will usher in a new paradigm for perioperative anticoagulation. Perioperative blood conservation in cardiac surgery recently has been highlighted ...
Jiang Ning - - 2012
To analyse the var gene repertoire and characterise the chondroitin sulphate A (CSA)-binding activity of the Duffy-binding like (DBL) domains encoded by the var2csa gene of a Plasmodium falciparum (P. falciparum) isolate in Hainan Province, China. The sequences of var DBL1 regions were PCR-amplified, sequenced and the sequence characteristics was ...
Mairet-Coello Georges - - 2012
During cerebral cortex development, precise control of precursor cell cycle length and cell cycle exit is required for balanced precursor pool expansion and layer-specific neurogenesis. Here, we defined the roles of cyclin-dependent kinase inhibitor (CKI) p57(KIP2), an important regulator of G1 phase, using deletion mutant mice. Mutant mice displayed macroencephaly ...
Kim Byunghyuk - - 2012
Mammalian male germ cells should be maintained below body temperature for proper development. Here, we investigated how male germ cells respond to heat stress. A short exposure of mouse testes to core body temperature induced phosphorylation of eIF2α and the formation of stress granules (SGs) in male germ cells. We ...
Lahner Edith - - 2012
Background:  Many micronutrients depend on a healthy stomach for absorption. Helicobacter pylori chronic gastritis may alter gastric physiology affecting homeostasis of vitamins and minerals. Objectives:  Systematic review to assess whether H. pylori infection is associated with reduced micronutrient levels (other than iron) in the plasma or gastric juice and whether low ...
Sallé Jérémy - - 2012
Endocycles, which are characterised by repeated rounds of DNA replication without intervening mitosis, are involved in developmental processes associated with an increase in metabolic cell activity and are part of terminal differentiation. Endocycles are currently viewed as a restriction of the canonical cell cycle. As such, mitotic cyclins have been ...
Ding Yi-Peng - - 2012
To investigate the prevalence and risk factors of bronchial asthma in the Li ethnic group in Hainan, China. This study employed a stratified random sample design using custom-designed questionnaires. Subjects with asthma-like symptoms were first identified by two rounds of surveys and then confirmed by respiratory physicians using pulmonary function ...
Mehta Alka - - 2012
To investigate the protective effects of hydroalcoholic and its fractions from roots of Hemidesmus indicus on arthritis in in vitro models of rodents. Preliminary phytochemical analysis and thin-layer chromoatography were performed to analyze constituents of hydroalcoholic extract and its three fraction namely ethyl acetate fraction, chloroform fraction and residual fraction ...
Spieth Peter M - - 2012
Chronic obstructive pulmonary disease (COPD) is a common cause of primary hospital admission and also a common coexisting disease among surgical patients. This translational review focused on recent studies related to the perioperative care of COPD patients. In addition to the crucial role of smoking cessation, the use of corticosteroids, ...
Perveen Rasida - - 2012
To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rosc (EEAC) rhizome against Ehrlich ascites carcinoma (EAC) tumor bearing Swiss Albino mice. In the present study, its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement, survival time, tumor cell growth inhibition, haematological characteristics etc. It ...
Toubert A - - 2012
Assessment of the host immune status is becoming a key issue in allogeneic hematopoietic stem cell transplantation (allo-HSCT). In the long-term follow-up of these patients, severe post-transplant infections, relapse or secondary malignancies may be directly related to persistent immune defects. In allo-HSCT, T-cell differentiation of donor progenitors within the recipient ...
Ezeja I Maxwell - - 2012
To investigate the antidiarrheal activity of the methanol leaf extract of Pterocarpus erinaceus in vivo. The methanol leaf extract of Pterocarpus erinaceus was evaluated using different doses (100, 200 and 400 mg/kg body weight) orally for antidiarrheal activity using castor oil-induced diarrhea, charcoal meal transit time and castor oil-induced enteropooling ...
Wan Mei H - - 2012
Acute pancreatitis (AP) is a formidable disease, which, in severe forms, causes significant mortality. Biliary AP, or gallstone obstruction-associated AP, accounts for 30-50% of all clinical cases of AP. In biliary AP, pancreatic acinar cell (PAC) death (the initiating event in the disease) is believed to occur as acinar cells ...
Hutton Melanie L - - 2012
Background:  Growth of Helicobacter pyloriin vitro depends on supplementation of the medium with blood or serum. However, these supplements often require frozen storage and can show batch-to-batch variation, resulting in differences in bacterial growth. In this study, we introduce the use of a commercially available, lipid-rich supplement called AlbuMAX II(®) ...
Silva Luciana C - - 2012
To describe high resolution manometry features of a population of symptomatic patients with Chagas' disease esophagopathy (CDE). Sixteen symptomatic dysphagic patients with CDE [mean age (54.81±13.43) years, 10 women] were included in this study. All patients underwent a high resolution manometry. Mean lower esophageal sphincter (LES) extension was (3.02±1.17) cm ...
Toledo Héctor - - 2012
Background:  Helicobacter pylori colonizes the gastric mucosa and must survive the acid pH of that environment. Like other enteric bacterial pathogens, including Salmonella enterica, H. pylori develops an acid tolerance response that is dependent on the function of the transcriptional regulator protein Fur. Objective:  To explore by site-directed mutagenesis whether two ...
Inbaneson Samuel Jacob - - 2012
To identify the possible antiplasmodial compounds from leaf, stem, root and flower extracts of Ocimum canum (O. canum), Ocimum sanctum (O. sanctum) and Ocimum basilicum (O. basilicum). The O. canum, O. sanctum and O. basilicum were collected from Ramanathapuram District, Tamil Nadu and the extraction was carried out in ethanol. ...
Moerer Onnen - - 2012
New developments in mechanical ventilation have focused on increasing the patient's control of the ventilator by implementing information on lung mechanics and respiratory drive. Effort-adapted modes of assisted breathing are presented and their potential advantages are discussed. Adaptive support ventilation, proportional assist ventilation with load adjustable gain factors and neurally ...
Guo Min - - 2012
To explore the characteristics of metabolic changes in patients with post-traumatic stress disorder through 1H-MRS in neuroanatomical circuit comparing with age-matches controls. Fifty patients with post-traumatic stress disorder and 50 gender-and age-matched normal controls were involved. The neurochemical abnormalities including the levels of choline (Cho)/creatine(Cr) and N-acetylaspartate (NAA)/Cr were measured ...
Todorovic Vesna V Department of Cell Biology, NYU Langone Medical Center, New York, New York 10016, USA. - - 2012
Latent transforming growth factor beta (TGF-β) binding proteins (LTBPs) are large extracellular glycoproteins structurally similar to fibrillins. They perform intricate and important roles in the extracellular matrix (ECM) and perturbations of their function manifest as a wide range of diseases. LTBPs are major regulators of TGF-β bioavailability and action. In ...
1 2 3 4 5 6 7 8 9 10 >