Search Results
Results 301 - 350 of 1016
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Bridenbaugh Eric A - - 2012
DNA microarray methodologies have proven to be an indispensable tool for genome-wide transcriptional profiling of organs, tissues, and cells. Here, we present a protocol for the optimized isolation and preparation of RNA from rat microvessels (including arteries, veins, and lymphatics) for subsequent use in two-color microarray analysis. The investigation of ...
Pang Ka-Lai - - 2012
In the Halosphaeriaceae, taxa with unfurling ascospore appendages and related species constitute 61 species (in 21 genera). Recent phylogenetic analyses of the rRNA genes have advanced our knowledge on the relationships between genera in the family, especially the group with unfurling ascospore appendages. However, many new genera resulting from these ...
Culver Bruce H - - 2012
Lung function parameters vary considerably with age and body size, so that, unlike many laboratory tests, the normal range of expected values must be individualized. For spirometry, only low values are considered to be abnormal, so the lower limit of normal (LLN) is taken to be equal to the 5th ...
Li Zhaoping - - 2012
Sarcopenia is a multifactorial age-related condition associated with a sedentary lifestyle and protein intakes during weight loss that are inadequate to maintain muscle mass. Sarcopenic obesity in the elderly is associated with a loss of independence and metabolic complications and represents a major public health challenge in individuals over the ...
Quaak Marieke - - 2012
Objectives Recent research strongly suggests that genetic variation influences smokers' ability to stop. Therefore, the use of (pharmaco) genetic testing may increase cessation rates. This study aims to assess the intention of smokers concerning undergoing genetic testing for smoking cessation and their knowledge, attitudes and preferences about this subject. Design ...
Masuda Megumi - - 2012
The objective of this study was to examine the effects of Morinda citrifolia (noni) extract and its constituents on α-melanocyte stimulating hormone (α-MSH)-stimulated melanogenesis in cultured murine B16 melanoma cells (B16 cells). A 50% ethanolic extract of noni seeds (MCS-ext) showed significant inhibition of melanogenesis with no effect on cell ...
Bubser Michael - - 2012
The muscarinic cholinergic system constitutes an important part of the neuronal circuitry that modulates normal cognition. Muscarinic receptor antagonists are well known to produce or exacerbate impairments in attention, learning, and memory. Conversely, both direct-acting muscarinic receptor agonists and indirect-acting muscarinic cholinergic agonists, such as acetylcholinesterase inhibitors, have shown cognition-enhancing ...
Bensen Daniel C - - 2012
One of the most important parameters correlated with success in protein crystallization experiments is sample purity and monodispersity. Heterologous expression systems have allowed investigators to produce engineered proteins in sufficient quantities which simplify the purification process compared with the days when macromolecules had to be extracted from source tissue. Improvements ...
Yamaura Katsunori - - 2012
Young green barley leaf is one of the richest sources of antioxidants and has been widely consumed for health management in Japan. In this study, we examined whether oral administration of young green barley leaf has an antidepressant effect on the forced swimming test in mice. Mice were individually forced ...
Ernst Joy Swanson - - 2012
This study compared 2 approaches to the assessment of Adult Protective Services (APS) cases. Using administrative data from 869 cases in 2 counties in Maryland, the differences between a nurse/social worker team and a lone social worker in case disposition, risk reduction, recidivism, and cost effectiveness were examined. Lone social ...
Percoco Marco - - 2012
In this note I reply to the comments by Haimes et al. on my paper on the sensitivity analysis of the inoperability input-output model. I make the case for a moment-independent sensitivity analysis.
Hamer Mariana - - 2012
We report a new method of immobilization of gold nanoparticles (AuNPs) on a fused-silica capillary through covalent binding. The resulting modified capillary was applied to electrophoretic systems to improve the efficiency of separation and the selectivity of selected solutes. The immobilization of AuNPs on the capillary wall was performed in ...
Cook Angela - - 2012
This study examined the prevalence, counts, and subtypes of Campylobacter, Salmonella, Listeria monocytogenes, verotoxigenic Escherichia coli (VTEC), and E. coli on raw retail chicken breast with the skin on versus the skin off. From January to December 2007, 187 raw skin-on chicken breasts and 131 skin-off chicken breasts were collected ...
Copin S - - 2012
The most-probable-number (MPN) method is often time-consuming for the isolation, detection, and quantification of Vibrio parahaemolyticus from natural sources. MPN counting of V. parahaemolyticus bacteria usually involves the isolation of typical V. parahaemolyticus colonies on selective medium, with subsequent confirmation by biochemical identification. In this study, we evaluated the use ...
Worthington Patricia H - - 2012
Parenteral nutrition is a life-saving modality, but one that also carries risks for potentially life-threatening complications. eomprehensivC management of patients receiving parenteral nutrition includes careful selection of candidates, individualizing formulas to meet patients' unique needs, monitoring response to therapy, and implementing strategies designed to avoid complications. Measures to mitigate the ...
Li Jieli - - 2012
The heart is the first organ to form and become functional in a developing embryo and its proper function is critical for most, if not all, subsequent stages of an animal's development. The formation of the heart relies heavily upon the rapid proliferation of cardiomyocytes at a specific stage during ...
McCormack Meredith C - - 2012
Single-breath diffusing capacity of the lung for carbon monoxide (D(LCO)) is a common pulmonary function test that measures the ability of the lung to exchange gas across the alveolar-capillary interface. D(LCO) testing is used to narrow the differential diagnosis of obstructive and restrictive lung disease, to aid in disability and ...
Braun U - - 2012
A 10-year-old Swiss Braunvieh cow near term was referred to our clinic because of severe abdominal distension, which caused loss of demarcation between the udder and ventral abdominal wall. Ultrasonographic examination revealed marked ascites and multiple echogenic nodules in the greater omentum. Based on the findings, non-inflammatory ascites attributable to ...
Wand Handan - - 2012
Objectives To investigate the impact of early sexual debut on HIV seroprevalence and incidence rates among a cohort of women. Design Prospective study. Setting KwaZulu-Natal, South Africa. Participants A total of 3492 sexually active women who consented to screen a HIV prevention trial during September 2002 to September 2005; a ...
Sato Shuichi - - 2012
We determined the binding affinity of tamsulosin, a selective α(1)-adrenoceptor antagonist, for human α(1)-adrenoceptor subtypes in comparison with those of other α(1)-adrenoceptor antagonists including silodosin, prazosin, 5-methylurapidil, terazosin, alfuzosin, nafopidil, urapidil and BMY7378. The association and dissociation kinetics of [(3)H]tamsulosin for recombinant human α(1)-adrenoceptor subtypes were compared with those of ...
Akiyama Mami - - 2012
Abstract A 62-year-old man with hypertension and diabetes mellitus controlled by medication suddenly noticed slight hemiparesis and was admitted to our hospital. Tissue-plasminogen activator (t-PA) was administered as his NIHSS was 6 and there were no contraindications. His symptoms completely resolved after t-PA injection. He was discharged on Day 9 ...
Wess Jürgen - - 2012
Muscarinic acetylcholine (ACh) receptors (mAChRs; M(1)-M(5)) regulate the activity of an extraordinarily large number of important physiological processes. During the past 10-15 years, studies with whole-body M(1)-M(5) mAChR knockout mice have provided many new insights into the physiological and pathophysiological roles of the individual mAChR subtypes. This review will focus ...
Bern-Klug Mercedes - - 2012
In a nationally representative study of 1,071 nursing home social services directors 80% of social services departments provided resident rights training and 60-70% were involved in abuse training. Departments headed by recent grads and in chain nursing homes or in the northeast were more likely to be involved in training. ...
Hnatiuk Oleh W - - 2012
Measurement of various aspects of pulmonary function is a relatively easy, noninvasive, and inexpensive way to gauge the status of the respiratory system. Interest in using these tests to determine risk from medical and surgical interventions stems from their presumed ability to be more sensitive than history or physical examination ...
Spindel Eliot R - - 2012
Many epithelial and endothelial cells express a cholinergic autocrine loop in which acetylcholine acts as a growth factor to stimulate cell growth. Cancers derived from these tissues similarly express a cholinergic autocrine loop and ACh secreted by the cancer or neighboring cells interacts with M3 muscarinic receptors expressed on the ...
Dombourian Melkon - - 2012
Fresh frozen plasma contains a number of therapeutically useful substances, most notably coagulation factors. As with any transfusion, there are risks associated with plasma transfusion. Ironically, the risk of viral transmission (human immunodeficiency virus or hepatitis), although widely publicized, is extremely small. On the other hand, less well-known, noninfectious complications ...
Saglio Giuseppe - - 2012
In patients with chronic myelogenous leukemia (CML) treated at diagnosis with the standard therapy consisting of imatinib, 400 mg once daily, the failure to achieve a complete cytogenetic response (CCyR) within 12 months from the start of therapy has been shown to be associated with an increased risk of progression ...
Stewart Martin P - - 2012
To understand the role of physical forces at a cellular level, it is necessary to track mechanical properties during cellular processes. Here we present a protocol that uses flat atomic force microscopy (AFM) cantilevers clamped at constant height, and light microscopy to measure the resistance force, mechanical stress and volume ...
Sridhar K R - - 2012
Three hundred and fifty woody litter and one hundred and forty seaweed litter sampled from seven beaches of Northwest Portugal were assessed for the filamentous fungal assemblage and diversity. The woody litter was screened for fungi up to 42 months using damp chamber incubation. They consisted of 36 taxa (ascomycetes, ...
Teilum Dorthe - - 2012
Background Vitamin D has been suggested to influence the incidence and prognosis of breast cancer, and studies have found better overall survival (OS) after diagnosis for breast cancer in summer-autumn, where the vitamin D level are expected to be highest. Objective To compare the prognostic outcome for early breast cancer ...
Niijima-Yaoita Fukie - - 2012
Exercise necessitates a large supply of O(2) and nutrients and rapid removal of CO(2) and waste products. Histamine is a regulator of the microcirculation (which performs these exchanges), suggesting a possible involvement of histamine in exercise. Histamine is released from either mast cells or non-mast cells. In the latter, histamine ...
Hillner Bruce E BE Department of Internal Medicine and the Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia 23298, USA. - - 2012
In July 2001, PET became a covered service for Medicare beneficiaries when used for the diagnosis, staging, and restaging of non-small-cell lung, esophageal, colorectal, and head and neck cancers as well as lymphoma and melanoma. Whether physicians use PET as a replacement for or in addition to CT, MRI, or ...
Rosoff Philip M - - 2012
Periodic and unexpected shortages of drugs, biologics, and even medical devices have become commonplace in the United States. When shortages occur, hospitals and clinics need to decide how to ration their available stock. When such situations arise, institutions can choose from several different allocation schemes, such as first-come, first-served, a ...
Coleman Shane M - - 2012
Just as in heart disease and diabetes, depression in HIV/AIDS is associated with negative outcomes. While randomized trials have shown the efficacy of treatment for depression in HIV/AIDS, the implementation of evidence-based treatments in real-world settings remains a challenge. The objective of this study was to assess the effectiveness of ...
Kunda Uma Maheswara Rao - - 2012
A green and efficient preparation method for the amino bisphosphonates is accomplished by simple mixing and stirring of diethylphosphite, triethylorthoformate and various amines in the presence of amberlyst-15 as catalyst at room temperature under solvent free conditions. The title compounds are characterized by IR, (1)H-, (13)C-, (31)P-NMR and mass spectra, ...
Liu Yi - - 2012
SDS is mostly used to enhance the solubilization and extraction of membrane proteins due to its strong detergency and low cost. Nevertheless, SDS interferes with the subsequent procedures and needs to be removed from the samples. In this work, a special gradient gel electrophoresis (GGE) system was developed to remove ...
Yang X - - 2012
Swab samples were obtained from groups of 25 carcasses at various stages of processing at a large beef packing plant. The log mean number of aerobes recovered from carcasses after skinning was 2.2 log CFU/cm(2). Spraying the uneviscerated carcasses with 5% lactic acid reduced the numbers of aerobes by about ...
Fu Andi - - 2012
Tube dislodgment is a common problem in drainage procedures. The present report compares a securement technique known as the bumper stitch, which aims to reduce tube motion and suture erosion, versus the traditional air knot. A total of 61 patients were enrolled, and 12 bumper stitches and 54 air knots ...
Zhang Yanping - - 2012
It is important to identify the mechanisms regulating cardiovascular development. However, complex genetic tools are often required, including transgenic animals that express the lacZ transgene encoding the β-galactosidase enzyme under the control of a specific promoter or following recombination with the Cre recombinase. The latter can be useful for identifying ...
Taniguchi Naoyuki - - 2012
A combination of the EMARS reaction and the application of mass spectrometry-based proteomics techniques promises to permit cell-surface molecular clustering to be analyzed under physiological conditions [Jiang et al., Proteomics 2012, 12, 54-62]. It is very likely that this approach will provide new insights into a wide range of research ...
Jandova V - - 2012
Despite the evidence for a genetic predisposition to develop equine sarcoids (ES), no whole genome scan for ES has been performed to date. The objective of this explorative study was to identify chromosome regions associated with ES. The studied population was comprised of two half-sibling sire families, involving a total ...
Verbout Norah G - - 2012
In this chapter, we will review what is known about muscarinic regulation of immune cells and the contribution of immune cell muscarinic receptors to inflammatory disease and immunity. In particular, immune cell expression of cholinergic machinery, muscarinic receptor subtypes and functional consequences of agonist stimulation will be reviewed. Lastly, this ...
Strauchler Daniel - - 2012
The aim of this study was to characterize cumulative exposure to ionizing radiation from diagnostic imaging (CEDI) in adult patients and investigate its relationship to a patient's socioeconomic status and comorbid medical conditions. A retrospective cohort study was conducted of 54,015 patients seen within the outpatient clinic system of an ...
Berry Elaine D - - 2012
Feedlot pen soil is a source for transmission of Escherichia coli O157:H7, and therefore a target for preharvest strategies to reduce this pathogen in cattle. The objective of this study was to determine the ability of soil solarization to reduce E. coli O157:H7 in feedlot surface material (FSM). A feedlot ...
Hove Jay R - - 2012
Due to its optical clarity and rudimentary heart structure (i.e., single atrium and ventricle), the zebrafish provides an excellent model for studying the genetic, morphological, and functional basis of normal and pathophysiological heart development in vivo. Recent advances in high-speed confocal imaging have made it possible to capture 2D zebrafish ...
Macintyre Neil R - - 2012
The pulmonary function lab of today is heavily focused on describing pathophysiology and quantifying the extent of disease. As we move forward, it is important that the results of pulmonary function tests go beyond this and be linked to important outcomes that truly affect clinical decision making. To get there, ...
Shanis Dana - - 2012
Female long-term survivors of allogeneic hematopoietic stem cell transplantation (allo-HSCT) incur a significant burden of late effects. Genital graft-versus-host disease (GVHD), human papillomavirus (HPV) reactivation, ovarian failure and infertility, sexual dysfunction, and osteoporosis are concerns that can significantly impact quality of life. This review examines the risk, pathogenesis, clinical presentation, ...
Kotani Hitoshi - - 2012
We investigated the properties of honokiol, a natural rexinoid, as a regulator of retinoid X receptor (RXR) heterodimers with various partner nuclear receptors (NRs), in comparison with those of the synthetic rexinoid bexarotene. Honokiol alone was hardly capable of activating peroxisome proliferator-activated receptor (PPAR) γ/RXR, RXR/liver X receptor (LXR), and ...
Nomura Sayo - - 2012
(±)-8-Deisopropyladunctin B, the deisopropyl form of adunctin B, which was isolated from the leaves of Piper aduncum (Piperaceae) collected in Papua New Guinea, was synthesized in 0.77% overall yield in 17 steps from 5,7-dimethoxycoumarin-3-carboxylate. The key step was our original stereoconvergent skeleton transformation from 1,2,2a,8b-tetrahydro-3H-benzo[b]cyclobuta[d]pyran-3-one to 1,2,4a,9b-tetrahydrodibenzofuran-4-ol with dimethylsulfoxonium methylide.
Giuliano Serena - - 2012
This work presents the proteome profile of cultured human skin fibroblasts established from a patient affected by DNA ligase I (Lig I) deficiency syndrome, a rare disorder characterized by immunodeficiency, growth retardation and sun sensitivity. 2-DE (in the 3-10 and 4-7 pH ranges) was the separation technique used for the ...
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