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Results 401 - 450 of 606
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- - 2009
Gaby, Alan - - 2009
Zablocki, Elaine - - 2009
Kohlstadt, Ingrid - - 2009
Klotter, Jule - - 2009
Klotter, Jule - - 2009
Klotter, Jule - - 2009
Klotter, Jule - - 2009
Klotter, Jule - - 2009
- - 2009
Collin, Jonathan - - 2009
Broggini, Giovanni - - 2009
Molecular markers derived from resistance gene analogs of HcrVf2, the first apple resistance gene cloned, may pave the way to the cloning of additional apple scab resistance genes. The Malus xdomestica 'Florina' (Vf) bacterial artificial chromosome (BAC) genomic library was screened by hybridization using HcrVJ2 as a probe. Positive BAC ...
Molnar, Istvan - - 2009
The frequency and pattern of irradiation-induced intergenomic chromosome rearrangements were analysed in the mutagenized ([M.sub.0]) and the first selfed ([M.sub.1]) generations of Triticum aestivum L.--Aegilops biuncialis Vis. amphiploids (2n = 70, AABBDD[U.sup.b][U.sup.b][M.sup.b]][M.sup.b]) by multicolour genomic in situ hybridization (mcGISH). mcGISH allowed the simultaneous discrimination of individual Ae. biuncialis genomes and ...
Shim, Youn-Seb - - 2009
Based on paper I in this series, our goals in this paper were to determine the relationship between prebombardment pretreatments and temperatures, microspore cell cycle when bombarded, and the frequencies of homozygous and hemizygous transgenic progeny in barley (Hordeum vulgare L.). Of the 104 fluorescent plants selected when using the ...
Shim, Youn-Seb - - 2009
The objective of this study was to determine when DNA synthesis occurred during pretreatments of cultured barley (Hordeum vulgare L.) microspores and during their preparation for particle bombardment. Based on this information, an investigation of the influence of cell cycle stage on the ability to obtain homozygous transgenic plants by ...
Durel, C.-E. - - 2009
Fire blight, caused by the bacterium Erwinia amylovora, is one of the most destructive diseases of apple (Malus xdomestica) worldwide. No major, qualitative gene for resistance to this disease has been identified so far in apple. A quantitative trait locus (QTL) analysis was performed in two Fl progenies derived from ...
Drosopoulou, Elena - - 2009
A Bactrocera oleae genomic library was constructed and several genomic clones bearing hsp70 sequences were isolated. All clones were in situ hybridized on the major heat shock puff locus of the salivary gland polytene chromosomes. Restriction mapping of the isolated clones and genomic Southern hybridization indicated the presence of several ...
Korban, Schuyler - - 2009
The genus Malus has anywhere between 25 and 33 species along with several subspecies. Malus species as well as clones within the same species have varying ploidy levels, as these are more than likely collected from different trees and (or) from different locations. In recent years, large numbers of Malus ...
Gupta, S.K. - - 2009
Genetic diversity in 20 elite blackgram (Vigna mungo (L.) Hepper) genotypes was studied using microsatellite and AFLP markers. Thirty-six microsatellite markers from azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) were successfully amplified across the 20 blackgram genotypes and 33 microsatellite markers showed polymorphism. A total of 137 microsatellite alleles ...
Oliveira, K.M. - - 2009
Expressed sequence tags (ESTs) offer the opportunity to exploit single, low-copy, conserved sequence motifs for the development of simple sequence repeats (SSRs). The authors have examined the Sugarcane Expressed Sequence Tag database for the presence of SSRs. To test the utility of EST-derived SSR markers, a total of 342 EST-SSRs, ...
Burow, Mark - - 2009
The cultivated peanut Arachis hypogaea is a tetraploid, likely derived from A-and B-genome species. Reproductive isolation of the cultigen has resulted in limited genetic variability for important traits. Artificial hybridizations using selected diploid parents have introduced alleles from wild species, but improved understanding of recently classified B-genome accessions would aid ...
Altaf, Mohammed - - 2009
The organization of the eukaryotic genome into chromatin makes it inaccessible to the factors required for gene transcription and DNA replication, recombination, and repair. In addition to histone-modifying enzymes and ATP- dependent chromatin remodeling complexes, which play key roles in regulating many nuclear processes by altering the chromatin structure, cells ...
Svotelis, Amy - - 2009
The mammalian genome is organized into a structure of DNA and proteins known as chromatin. In general, chromatin presents a barrier to gene expression that is regulated by several pathways, namely by the incorporation of histone variants into the nucleosome. In yeast, H2A.Z is an H2A histone variant that is ...
Dalal, Yamini - - 2009
Centromeres are the discrete sites of spindle microtubule attachment on chromosomes during mitosis and meiosis in all eukaryotes. These highly specialized chromatin structures typically occupy the same site for thousands of generations, yet the mechanism by which centromeres are established, maintained, and function remain a mystery. In metazoans, centromeric DNA ...
Gieni, Randall - - 2009
Actin exists as a dynamic equilibrium of monomers and polymers within the nucleus of living cells. It is utilized by the cell for many aspects of gene regulation, including mRNA processing, chromatin remodelling, and global gene expression. Polymeric actin is now specifically linked to transcription by RNA polymerase I, II, ...
Yang, Hongbo - - 2009
Antisera raised against individual sites of histone post-translational modification (PTM) have provided critical insights into the biology of many of these PTMs. However, limitations inherent to immunochemical approaches can skew results obtained with these reagents, possibly leading investigators to misjudge the role of a specific histone PTM in a given ...
Shi, Yongquan - - 2009
The haemopoietic cell kinase (Hck) plays an important but poorly understood role in coupling chemoattractant stimuli to the actin cytoskeletal rearrangement required for neutrophil polarization and chemotaxis. Here, we show that Hck coimmunoprecipitates with the cytoskeletal regulatory Wiskott-Aldrich syndrome protein (WASp) and mammalian diaphanous- related formin 1 (mDia1) in chemoattractant-stimulated ...
Adams-Cioaba, Melanie - - 2009
Chromatin structure is regulated by chromatin remodeling factors, histone exchange, linker histone association, and histone modification. Covalent modification of histones is an important factor in the regulation of the associated processes. The implementation and removal of various histone modifications have been implicated in DNA replication, repair, recombination, and transcription, and ...
Tremblay, Maxime - - 2009
The genome is organized into nuclear domains, which create microenvironments that favor distinct chromatin structures and functions (e.g., highly repetitive sequences, centromeres, telomeres, noncoding sequences, inactive genes, RNA polymerase II and III transcribed genes, and the nucleolus). Correlations have been drawn between gene silencing and proximity to a heterochromatic compartment. ...
Raghuram, Nikhil - - 2009
The histone H1 family of nucleoproteins represents an important class of structural and architectural proteins that are responsible for maintaining and stabilizing higher-order chromatin structure. Essential for mammalian cell viability, they are responsible for gene-specific regulation of transcription and other DNA-dependent processes. In this review, we focus on the wealth ...
Kelbauskas, L. - - 2009
Forster resonance energy transfer (FRET) techniques provide powerful and sensitive methods for the study of conformational features in biomolecules. Here, we review FRET-based studies of nucleosomes, focusing particularly on our work comparing the widely used nucleosome standard, 5S rDNA, and 2 promoter-derived regulatory element-containing nucleosomes, mouse mammary tumor virus (MMTV)-B ...
Lavelle, Christophe - - 2009
Genomic DNA in eukaryotic cells is organized in discrete chromosome territories, each consisting of a single huge hierarchically supercoiled nucleosomal fiber. Through dynamic changes in structure, resulting from chemical modifications and mechanical constraints imposed by numerous factors in vivo, chromatin plays a critical role in the regulation of DNA metabolism ...
Mellone, Barbara - - 2009
Normal inheritance of genetic material requires that chromosomes segregate faithfully during mitosis and meiosis. The kinetochore is a unique structure that attaches chromosomes to the microtubule spindle, monitors proper chromosome attachment to the spindle through the mitotic checkpoint, and couples spindle and motor protein forces to move chromosomes during prometaphase ...
Riddle, Nicole - - 2009
The fourth chromosome of Drosophila melanogaster has a number of unique properties that make it a convenient model for the study of chromatin structure. Only 4.2 Mb overall, the 1.2 Mb distal arm of chromosome 4 seen in polytene chromosomes combines characteristics of heterochromatin and euchromatin. This domain has a ...
Draker, Ryan - - 2009
The chromatin organization of a genome ultimately dictates the gene expression profile of the cell. It is now well recognized that key mechanisms that regulate chromatin structure include post-translational modifications of histones and the incorporation of histone variants at strategic sites within the genome. H2A.Z is a variant of H2A ...
Hite, Kristopher - - 2009
Mutations in methyl DNA binding protein 2 (MeCP2) cause the neurodevelopmental disorder Rett syndrome (RTT). The mechanism(s) by which the native MeCP2 protein operates in the cell are not well understood. Historically, MeCP2 has been characterized as a proximal gene silencer with 2 functional domains: a methyl DNA binding domain ...
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